Nucleolar organization in oocytes of gryllid crickets: Subfamilies gryllinae and nemobiinae

Allen, E. Raworth; Cave, M. Donald

doi:10.1002/jmor.1051370404

Cited by 13 publications

(2 citation statements)

References 19 publications

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“…Failure to detect genes coding for rRNA in the nuclei of somatic cells, in spite of the fact that 0 .004% of the DNA in somatic tissues hybridizes with rRNA, indicates that the number of ribosomal genes in somatic MAC DONALD CAVE Localization of Ribosomal DNA in Oocytes of House Cricket 1956 ; Lima-de-Faria and Moses, 1966), and Coleoptera (Urbani, 1969 ;Urbani and Russo-Caia, 1964 ;Kato, 1969 ;Bier et al ., 1967 ; . In oocytes of Orthopterans, DNA which is present in addition to the chromosomes is closely associated with the oocyte nucleoli of several species of grasshoppers belonging to the family Acrididae (Kunz, 1967), and of crickets (Gryllidae) belonging to the subfamilies Gryllinae and Nemobiinae (Johnson, 1938 ;Favard-Sereno, 1968 ;Heinonen and Halkka, 1967 ; b ; Kunz, 1969 ;Allen and Cave, 1972) .…”

Section: Resultsmentioning

confidence: 99%

“…reuther, 1957), Diptera (Bauer, 1933 ;Bayreuther, 1956 ;Lima-de-Faria and Moses, 1966), and Coleoptera (Urbani, 1969 ;Urbani and Russo-Caia, 1964 ;Kato, 1969 ;Bier et al ., 1967 ; . In oocytes of Orthopterans, DNA which is present in addition to the chromosomes is closely associated with the oocyte nucleoli of several species of grasshoppers belonging to the family Acrididae (Kunz, 1967), and of crickets (Gryllidae) belonging to the subfamilies Gryllinae and Nemobiinae (Johnson, 1938 ;Favard-Sereno, 1968 ;Heinonen and Halkka, 1967 ;Cave and Allen, 1969 b ;Kunz, 1969 ;Allen and Cave, 1972) .…”

Section: Figurementioning

confidence: 99%

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LOCALIZATION OF RIBOSOMAL DNA WITHIN OOCYTES OF THE HOUSE CRICKET, ACHETA DOMESTICUS (ORTHOPTERA: GRYLLIDAE)

Cave

1972

The Journal of Cell Biology

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A large DNA-containing body is present in addition to the chromosomes in oocytes of the house cricket Acheta domesticus. Large masses of nucleolar material accumulate at the periphery of the DNA body during the diplotene stage of meiotic prophase I . RNA-DNA hybridization analysis demonstrates that the genes which code for 18S and 28S ribosomal RNA are amplified in the ovary . In situ hybridization indicates that the amplified genes are localized within the DNA body of early prophase cells . As the cells proceed through diplotene the DNA which hybridizes with ribosomal RNA is gradually incorporated into the developing nucleolar mass .1933 ; Bayreuther, 1956 ;Urbani, 1969) . Such a structure, referred to as the DNA body, is present in oocytes of the house cricket, Acheta domesticus (Nilsson, 1966) . The DNA body is limited to the oocytes and is not present in the nuclei of somatic cells . Microphotometric determinations made on the nuclei of Feulgen-stained pachytene stage cells show that the DNA body is present in addition to chromosomal DNA and accounts for approximately 25% of the DNA in the pachytene stage oocyte (Cave and Allen, 1969 a) . During diplotene large masses of RNA-containing material accumulate at the periphery of the DNA body (Heinonen and Halkka, 1967 ;Lima-de-Faria et al ., 1968) . Electron microscope examination indicates that the RNA-containing material associated with the body is characteristically nucleolar in appearance, the granular elements measuring 200-250 A in diameter (Allen and Cave, 1968 ; Cave and Allen, 1969 b ;Kunz, 1969) . Treatment with actinomycin D causes a typical segregation of components into

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Section: Resultsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

LOCALIZATION OF RIBOSOMAL DNA WITHIN OOCYTES OF THE HOUSE CRICKET, ACHETA DOMESTICUS (ORTHOPTERA: GRYLLIDAE)

Cave

1972

The Journal of Cell Biology

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Nucleolar DNA in oocytes of crickets: Representatives of the subfamilies Oecanthinae and Gryllotalpinae (Orthoptera: Gryllidae)

Cave

Allen

1974

Journal of Morphology

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A large extrachromosomal mass of Feulgen positive material, the DNA body, has been visualized in early prophase oocytes of crickets (Orthoptera: Gryllidae) representative of the closely related subfamilies Gryllinae and Nemobiinae. A similar structure is present in oocytes of representatives of two subfamilies of crickets (subfamilies Oecanthinae and Gryllotalpinae) which taxonomically and phylogenetically are quite separate from those mentioned previously. In situ hybridization demonstrates that the body contains amplified copies of genes coding for ribosomal RNA. Unlike the DNA body in early diplotene oocytes of representatives of thc subfamily Gryllinae, which is closely associated with the developing nucleolar apparatus, the DNA body in oocytes of the Oecanthinae and Gryllotalpinae cannot be demonstrated during diplotene. In the Oecanthinae, the nucleolar apparatus of early diplotene stage oocytes is composed of four to seven separate structures, the ribonucleoprotein of which has a characteristically lamellated appearance. During late diplotene, these nucleoli give rise to many smaller structures which are distributcd throughout the germinal vesicle. In early diplotene stage oocytes of Scqpteriscus acletus (Subfamily: Gryllotalpinae), the nucleolar apparatus coiisists of a single compact mass of ribonucleoprotein. In contrast to the oocytes of all other crickets that have been studied, the nucleolus of S. acletus remains single throughout diplotene. I n situ hybridization analysis indicates that the amplified genes coding for rRNA which are localized in the DNA body of early prophase oocytes become incorporated into this compact nucleolar mass. Differences in nucleolar structure appear to reflect differences in the organization of amplified genes coding for rRNA.

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Morphological Manifestations of Ribosomal DNA Amplification during Insect Oogenesis

Cave

1982

Insect Ultrastructure

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Nucleolar organization in oocytes of gryllid crickets: Subfamilies gryllinae and nemobiinae

Cited by 13 publications

References 19 publications

LOCALIZATION OF RIBOSOMAL DNA WITHIN OOCYTES OF THE HOUSE CRICKET, ACHETA DOMESTICUS (ORTHOPTERA: GRYLLIDAE)

LOCALIZATION OF RIBOSOMAL DNA WITHIN OOCYTES OF THE HOUSE CRICKET, ACHETA DOMESTICUS (ORTHOPTERA: GRYLLIDAE)

Nucleolar DNA in oocytes of crickets: Representatives of the subfamilies Oecanthinae and Gryllotalpinae (Orthoptera: Gryllidae)

Morphological Manifestations of Ribosomal DNA Amplification during Insect Oogenesis

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