Nucleolar DEAD-Box RNA Helicase TOGR1 Regulates Thermotolerant Growth as a Pre-rRNA Chaperone in Rice

Wang, Dong; Qin, Baoxiang; Li, Xiang; Tang, Ding; Zhang, Yue; Cheng, Zhukuan; Xue, Yongbiao

doi:10.1371/journal.pgen.1005844

Cited by 93 publications

(87 citation statements)

References 68 publications

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“…Wang et al . () also demonstrated that genetic variations in ZmVPP1 contribute to drought tolerance in maize seedlings. In the present research, we found that inbred lines with Hap3 had a significantly longer kernel length than those with Hap1 and Hap2 on the basis of candidate gene‐based association analysis using data from a panel of 479 maize genotypes.…”

Section: Discussionmentioning

confidence: 89%

“…In Arabidopsis, both P-A3 and 32S pre-rRNA intermediates can be detected during pre-rRNA processing, indicating that the two pre-rRNA processing pathways also co-exist in this plant (Hang et al, 2014;Weis et al, 2015b). In rice, however, only the intermediate of P-A3 has been detected, suggesting that the 'preferential cleavage in ITS1 before the complete removal of the 5 0 ETS' is the main pathway for pre-rRNA processing (Wang et al, 2016a). In the present research, we detected the intermediates of both P-A3 and 32S rRNA in maize by northern blot and/or cRT-PCR, demonstrating that the two pre-rRNA processing pathways co-exist in maize.…”

Section: Discussionmentioning

confidence: 98%

“…The limited evidence suggests that RBFs have a regulatory role in plant growth and development. For example, TOGR1 (Thermotolerant Growth Required 1), a DEAD-box RNA helicase that functions as a key chaperone for rRNA biogenesis, is required for rice thermotolerance, and TOGR1 overexpression significantly improves rice growth under hot conditions (Wang et al, 2016a). An Arabidopsis C/D box snoRNA, HIDDEN TREASURE 2 (HID2), is associated with the precursor 45S rRNA and promotes the efficiency and accuracy of pre-rRNA processing; knockdown of HID2 impairs normal growth and development of Arabidopsis .…”

Section: Introductionmentioning

confidence: 99%

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Maize Urb2 protein is required for kernel development and vegetative growth by affecting pre‐ribosomal RNA processing

Wang

et al. 2018

New Phytologist

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Ribosome biogenesis is a fundamental process in eukaryotic cells. Although Urb2 protein has been implicated in ribosome biogenesis in yeast, the Urb2 domain is loosely conserved between plants and yeast, and the function of Urb2 protein in plants remains unknown. Here, we isolated a maize mutant, designated as urb2, with defects in kernel development and vegetative growth. Positional cloning and transgenic analysis revealed that urb2 encodes an Urb2 domain-containing protein. Compared with the wild-type (WT), the urb2 mutant showed decreased ratios of 60S/40S and 80S/40S and increased ratios of polyribosomes. The pre-rRNA intermediates of 35/33S rRNA, P-A3 and 18S-A3 were significantly accumulated in the urb2 mutant. Transcriptome profiling of the urb2 mutant indicated that ZmUrb2 affects the expression of a number of ribosome-related genes. We further demonstrated that natural variations in ZmUrb2 are significantly associated with maize kernel length. The overall results indicate that, by affecting pre-rRNA processing, the Urb2 protein is required for ribosome biogenesis in maize.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Maize Urb2 protein is required for kernel development and vegetative growth by affecting pre‐ribosomal RNA processing

Wang

et al. 2018

New Phytologist

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“…In the Neurospora clock, FRQ-interacting RNA Helicase (FRH)’s primary role is to mediate a proper contact among FRQ, CK1a and WCC (Hurley et al, 2013; Lauinger et al, 2014; Conrad et al, 2016). TOGR1, a DEAD-box helicase in the rice plant, is involved in temperature-dependent and clock-controlled plant growth through stabilizing rRNA homeostasis (Wang et al, 2016). DDX5, an ortholog of PRD-1, is associated in the PER complex, and regulates per RNA metabolism and its clock function (Padmanabhan et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Experimental and Mathematical Analyses Relating Circadian Period and Phase of Entrainment inNeurospora crassa

et al. 2017

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Circadian rhythms are observed in most organisms on earth and known to play a major role in successful adaptation to the 24 h cycling environment. Circadian phenotypes are characterized by a free running period that is observed in constant conditions and an entrained phase that is observed in cyclic conditions. Thus, the relationship between the free running period and phase of entrainment is of interest. A popular simple rule has been that the entrained phase is the expression of the period in a cycling environment, i.e., that a short period causes an advanced phase, and a long period causes a delayed phase. However, there are experimental data that are not explained by this simple relationship, and no systematic study has been done to explore all possible period-phase relationships. Here we show the existence of stable period-phase relationships that are exceptions to this rule. First, we analyzed period-phase relationships using populations with different degrees of genome complexity. Second, we generated isogenic F1 populations by crossing 14 classical period mutants to the same female, and analyzed two populations with a short period/delayed phase and a long period/advanced phase. Third, we generated a mathematical model to account for such variable relationships between period and phase. Our analyses support the view that the circadian period of an organism is not the only predictor of the entrained phase.

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“…DEAD-box proteins comprise a large protein family and are involved in almost all nucleic acid metabolic activities, including replication, repair, recombination, transcription, translation, ribosome biogenesis, and splicing [47,48]. It has been reported that the DEAD-box proteins play important roles in plant growth and development, as well as abiotic (10 of 12) 1600365 stress response [49][50][51]. In the present study, we reported that DEAD-box ATP-dependent RNA helicase 20 is involved in rice response to HNT stress, and RNA helicase 20 was upregulated by over 1.7-fold in the HTL and downregulated by 1.5-fold (1/0.658) in the HSL, compared to the controls, respectively.…”

Section: Transcript Regulation Proteins Were Upregulated In the Htl Amentioning

confidence: 99%

Quantitative iTRAQ‐based proteomic analysis of rice grains to assess high night temperature stress

et al. 2017

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Rice yield and quality are adversely affected by increasing global surface temperature, and are strongly attributed to high night temperature (HNT) than high daytime temperature. However, the molecular mechanism underlying the heat‐tolerant characteristics of rice remains unclear. In the present study, we compared the proteomes of heat‐tolerant and ‐sensitive lines of rice at early milky stage using an iTRAQ method. We have identified 38 differentially expressed proteins between the two lines, of which 32 proteins have been functionally annotated in NCBI and/or the UniProt database. These proteins were then classified into seven functional subgroups, which include signal transduction, transcript regulation, oxidation, defense response, transport, energy metabolism, and biosynthesis. Further analysis indicated that HNT stress could disrupt the redox equilibrium of plant cells, which in turn triggers the calcium‐dependent protein kinase and COP9 signalosome, thereby regulating downstream genes/proteins that are involved in the HNT response. The candidate proteins may provide genetic resources for the improvement of heat‐tolerant characteristics in rice, and the proposed model for signal transduction and transcriptional regulation may facilitate in the elucidation of the molecular mechanism underlying the response to HNT stress in rice.

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Nucleolar DEAD-Box RNA Helicase TOGR1 Regulates Thermotolerant Growth as a Pre-rRNA Chaperone in Rice

Cited by 93 publications

References 68 publications

Maize Urb2 protein is required for kernel development and vegetative growth by affecting pre‐ribosomal RNA processing

Maize Urb2 protein is required for kernel development and vegetative growth by affecting pre‐ribosomal RNA processing

Experimental and Mathematical Analyses Relating Circadian Period and Phase of Entrainment inNeurospora crassa

Quantitative iTRAQ‐based proteomic analysis of rice grains to assess high night temperature stress

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