Nucleolar and spindle associated protein 1 promotes metastasis of cervical carcinoma cells by activating Wnt/β-catenin signaling

Han, Lei; Zhang, Weijing; Yan, Ming; Qiu, Jiaqi; Chen, Jueming; Sun, Xiaoying; Chen, Xiangfu; Song, Libing; Zhang, Yanna

doi:10.1186/s13046-019-1037-y

Cited by 71 publications

(86 citation statements)

References 54 publications

(53 reference statements)

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“…[3][4][5] Increased expression of NUSAP1 has been observed in many tumors, such as prostate cancer, acute myeloid leukemia, cervical cancer, oral squamous cell carcinoma, and astrocytoma, and NUSAP1 has been found to be closely associated with tumor progression. [6][7][8][9][10] However, the potential relationship between NUSAP1 expression in BLCA and clinical outcomes remains unknown and requires further investigation.…”

Section: Introductionmentioning

confidence: 99%

<p>Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-β Signaling Pathway</p>

Gao

Yin

Tong

et al. 2020

OTT

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Purpose: NUSAP1 has been reported to be involved in the progression of several types of cancer. However, its expression and exact role in bladder cancer (BLCA) remains elusive. The aim of this study was to determine the expression and role of NUSAP1 in BLCA. Methods: Tissue microarray, real-time PCR, Western blot and immunohistochemistry assays were carried out to determine NUSAP1 expression in BLCA tissues and cells. The biological roles of NUSAP1 were investigated using CCK-8, EdU labeling, flow cytometry, Transwell, and wound healing assays. Additionally, the effect of NUSAP1 on epithelialmesenchymal transition (EMT) was investigated by Western blotting and real-time PCR. Results: We found that NUSAP1 was upregulated in BLCA, and its expression was closely related to the poor prognosis of patients. Subsequently, we transfected 5637 and T24 cell lines with NUSAP1 siRNA and an NUSAP1 overexpression plasmid, respectively. NUSAP1 downregulation in 5637 cells inhibited cell proliferation, migration, and invasiveness and enhanced chemosensitivity to gemcitabine, while NUSAP1 overexpression in T24 cells resulted in the inverse effects. Moreover, NUSAP1 regulated EMT via the TGF-β signaling pathway, and when TGF-beta receptor 1 (TGFBR1) was inhibited with the inhibitor SB525334, the invasion and metastasis ability of BLCA cells was significantly suppressed, as well as p-Smad2/3 and vimentin expression. Conclusion: Our above data demonstrate that NUSAP1 contributes to BLCA progression via the TGF-β signaling pathway.

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Section: Introductionmentioning

confidence: 99%

<p>Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-β Signaling Pathway</p>

Gao

Yin

Tong

et al. 2020

OTT

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“…The expression of NUSAP1 is frequently increased in the proliferating cells, leading to microtubule bundling and G2/M phase arrest . Recently, reposts have demonstrated that NUSAP1 is strongly implicated in the tumorigenicity of several kinds of cancers, such as cervical carcinoma, breast cancer, and liver cancer . In bladder cancer, Pan et al reported that NUSAP1 was overexpressed in bladder cancer and play crucial roles in the maintain of stemness in bladder cancer cells.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer

Chen

Zhang

Kadier

et al. 2020

Clinical Laboratory Analysis

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Background Nucleolar and spindle‐associated protein 1 (NUSAP1) has been identified to be strongly implicated in the carcinogenesis of cervical carcinoma, breast cancer, and liver cancer, and shows a high expression level in bladder cancer, indicating that NUSAP1 might be a potent target for cancer treatment. Using bioinformatics methods, we found that NUSAP1 was a putative target of miR‐769‐5p. Here, we aimed to explore whether miR‐769‐5p is involved in bladder cancer progression via targeting NUSAP1. Methods MiR‐769‐5p expression patterns in bladder cancer tissues and cells were detected by RT‐PCR. Kaplan‐Meier was used to determine the clinical effects of miR‐769‐5p expression levels on the overall survival of bladder cancer patients. Bioinformatics methods were used to predict the binding sites between miR‐769‐5p and NUSAP1, which was verified by the luciferase gene reporter assay. CCK‐8, flow cytometry, wound healing and transwell chamber experiments were performed to test cell growth, apoptosis, migration and invasion capacities. Results miR‐769‐5p was lowly expressed in bladder cancer tissues and cells, which was closely associated with poor prognosis. Overexpression of miR‐769‐5p induced significant repressions in cell growth, migration, and invasion and caused an obvious increase in cell apoptosis, whereas these tendencies were reversed when NUSAP1 was upregulated. Conclusion This study demonstrates that miR‐769‐5p functions as a tumor suppressor in bladder cancer via targeting NUSAP1.

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“…Recently, accumulating evidence has indicated that dysregulation of NUSAP1 is closely associated with tumour progression, chemoresistance, and patients survival. In cervical carcinoma, Li et al illuminated that NUSAP1 could promote cancer stem cell properties and epithelial‐mesenchyme transition (EMT) through activing Wnt/β‐catenin signalling, thereby accelerating cell migration and invasion. Okamoto et al revealed high NUSAP1 expression was related to more advanced T stage and inhibiting NUSAP1 resulted in the suppression of cell proliferation in Oral Squamous Cell Carcinoma (OSCC).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of NUSAP1 suppresses cell proliferation, migration, and invasion via inhibiting mTORC1 signalling pathway in gastric cancer

Lin

et al. 2019

Cell Biochemistry & Function

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Gastric cancer (GC) is one of the most common causes of cancer-related death worldwide, and outstanding biomarkers for therapeutic targets or predicting GC survival are still lacking. Increasing evidence indicated that nucleolar and spindle associated protein 1 (NUSAP1) involved in regulating the progression of various cancers; however, its specific role in GC remained unclear. In this study, we found that NUSAP1 was upregulated in the GC tissues and cell lines via analysing data from The Cancer Genome Atlas (TCGA), gene expression omnibus (GEO), qRT-PCR, and western blot assays. Patients with high NUSAP1 expression levels showed shorter freeprogression survival (FPS), larger tumour size, and higher lymphatic metastasis rate compared with those with low NUSAP1 expression. Further functional experiments revealed knockdown of NUSAP1 could inhibit the growth, migration, and invasion of GC cells in vitro and vivo. Additionally, silencing NUSAP1 induced G0/G1 phase arrest, apoptosis, and suppressed the epithelial-mesenchymal transition (EMT) process. Finally, we performed gene set enrichment analysis (GSEA) and observed NUSAP1 was positive with mTORC1 signalling pathway, which was verified by the subsequent immunoblotting. In conclusion, our findings suggested that NUSAP1 contributed to GC progression and may act as a potential therapeutic target for GC.Significance of the study: Our results firstly illuminated that NUSAP1 expression was significantly upregulated in GC tissues and predicted poor FPS. Silencing it could attenuate GC progression via inhibiting mTORC1 signalling pathway. Hence, NUSAP1 may act as a promising therapy target for GC.

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Nucleolar and spindle associated protein 1 promotes metastasis of cervical carcinoma cells by activating Wnt/β-catenin signaling

Cited by 71 publications

References 54 publications

<p>Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-β Signaling Pathway</p>

<p>Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-β Signaling Pathway</p>

MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer

Downregulation of NUSAP1 suppresses cell proliferation, migration, and invasion via inhibiting mTORC1 signalling pathway in gastric cancer

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