&lt;p&gt;Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-β Signaling Pathway&lt;/p&gt;

Gao, Shun; Yin, Hubin; Tong, Hang; Zhan, Kai; Yang, Guang; Hossain, Mohammad Arman; Li, Tinghao; Gou, Xin

doi:10.2147/ott.s237127

Cited by 23 publications

(24 citation statements)

References 28 publications

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“…22 In addition, the involvement of NUSAP1 in bladder cancer has been demonstrated on account of the effects of NUSAP1 knockdown or overexpression on cell proliferation, migration, invasiveness and chemosensitivity to gemcitabine by regulating the activation of TGF-β Signaling Pathway. 23 In the present study, we examined NUSAP1 expression in some osteosarcoma cell lines and discovered the upregulation of NUSAP1 in these cells compared with normal osteoblast cells, which consistent with previously published findings. However, we did not explore the expression of NUSAP1 gene in bone mesenchymal cells-derived tumor cells and we are exploring the expression and biological effects of NUSAP1 in bone mesenchymal cellsderived tumor cell lines and investigating the potential mechanism in our next study.…”

Section: Dovepresssupporting

confidence: 92%

NUSAP1 Accelerates Osteosarcoma Cell Proliferation and Cell Cycle Progression via Upregulating CDC20 and Cyclin A2

Wang¹,

Liu²,

Wu³

et al. 2021

OTT

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Purpose: Nucleolar and spindle-associated protein 1 (NUSAP1) is a significant mitotic regulator and has been found to be implicated in carcinogenesis of several cancers. The aim of this study was to explore the functional role and underlying mechanisms of NUSAP1 in osteosarcoma. Methods: Western blot assay and Real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) were employed to assess the expressions of NUSAP1, cell division cycle 20 homologue (CDC20) and cyclin A2 (CCNA2) in osteosarcoma cells. Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8) assay and 5-ethynyl-2′-deoxyuridine (EdU) assay, and flow cytometry was applied for exploring cell cycle. In addition, an osteosarcoma tumor-bearing mouse model was established by injection of transfected osteosarcoma cells. Tumor volume and protein expressions of Ki67 and PCNA were examined. Bioinformatics analysis and immunoprecipitation were used to identify the combination of NUSAP1 with CDC20 and CCNA2. Results: The mRNA and protein expression of NUSAP1 were extremely upregulated in osteosarcoma cells. Overexpression of NUSAP1 promoted whereas NUSAP1 silencing suppressed cell proliferation and cell cycle progression in transfected osteosarcoma cells. In osteosarcoma mouse model, NUSAP1 expression affected tumor volume and levels of Ki67 and PCNA. Moreover, CDC20 or CCNA2 silencing inhibited NUSAP1-induced cell proliferation and cell cycle in osteosarcoma cells. Conclusion:Our data demonstrated that upregulated NUSAP1 may exacerbate the development of osteosarcoma by accelerating the proliferation and cell cycle process of osteosarcoma cells by binding to CDC20 and CCNA2, suggesting NUSAP1 as a possible therapeutic target for treatment of osteosarcoma.

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Section: Dovepresssupporting

confidence: 92%

NUSAP1 Accelerates Osteosarcoma Cell Proliferation and Cell Cycle Progression via Upregulating CDC20 and Cyclin A2

Wang¹,

Liu²,

Wu³

et al. 2021

OTT

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“…For instance, in cervical cancer ( Li et al, 2019 ), NUSAP1 is shown to bound to the SUMO-E3 ligase Ran binding protein 2 (RanBP2) to induce the sumoylation of TCF4, thereby enhancing the Wnt/β signaling pathway and inducing tumor metastasis. Similarly, a high NUSAP1 expression level facilitated the development of bladder cancer by regulating epithelial-mesenchymal transition of bladder cancer via the TGF-β signaling pathway ( Gao S. et al, 2020 ). In addition, Liu ( Liu et al, 2018 ) reports that the mRNA and protein levels of NUSAP1 were more highly expressed in colon cancer than in paired non-cancerous adjacent tissues.…”

Section: Discussionmentioning

confidence: 99%

Four Novel Prognostic Genes Related to Prostate Cancer Identified Using Co-expression Structure Network Analysis

Feng

Wei

et al. 2021

Front. Genet.

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Prostate cancer (PCa) is one of the most common malignancies for males, but very little is known about its pathogenesis. This study aimed to identify novel biomarkers associated with PCa prognosis and elucidate the underlying molecular mechanism. First, The Cancer Genome Atlas (TCGA) RNA-sequencing data were utilized to identify differentially expressed genes (DEGs) between tumor and normal samples. The DEGs were then applied to construct a co-expression and mined using structure network analysis. The magenta module that was highly related to the Gleason score (r = 0.46, p = 3e–26) and tumor stage (r = 0.38, p = 2e–17) was screened. Subsequently, all genes of the magenta module underwent function annotation. From the key module, CCNA2, CKAP2L, NCAPG, and NUSAP1 were chosen as the four candidate genes. Finally, internal (TCGA) and external data sets (GSE32571, GSE70770, and GSE141551) were combined to validate and predict the value of real hub genes. The results show that the above genes are up-regulated in PCa samples, and higher expression levels show significant association with higher Gleason scores and tumor T stage. Moreover, receiver operating characteristic curve and survival analysis validate the excellent value of hub genes in PCa progression and prognosis. In addition, the protein levels of these four genes also remain higher in tumor tissues when compared with normal tissues. Gene set enrichment analysis and gene set variation analysis for a single gene reveal the close relation with cell proliferation. Meanwhile, 11 small molecular drugs that have the potential to treat PCa were also screened. In conclusion, our research identified four potential prognostic genes and several candidate molecular drugs for treating PCa.

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“…Nucleolar spindle-associated protein 1 (NuSAP1) is a microtubule-binding protein that participates in the regulation of mitotic progression and spindle formation and stability [13]. Recent studies have found that NuSAP1 was overexpressed in several types of cancers such as prostate cancer, colorectal cancer, and astrocytoma [14][15][16]. NuSAP1 also served as a key regulator of breast cancer progression, and it contributed to cell proliferation and invasion [17].…”

Section: Introductionmentioning

confidence: 99%

ANKRD22 enhances breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression

Liu

Gong

et al. 2020

Bosn J of Basic Med Sci

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Breast cancer is one of the most prevalent malignancies in women worldwide. Although great progress has been achieved in the diagnosis and treatment of breast cancer, the prognosis of patients with breast cancer is still poor due to distal recurrence and metastasis after surgery. This study demonstrated that the expression level of ankyrin repeat domain 22 (ANKRD22) in human breast cancer tissues was significantly higher than that in normal breast tissues. ANKRD22 knockdown inhibited the proliferation, invasion, and epithelial-mesenchymal transition (EMT) of breast cancer cells. Further research indicated that ANKRD22 regulated the expression of nucleolar and spindle-associated protein 1 (NuSAP1) and then the activation of Wnt/β-catenin signaling pathway. Moreover, overexpression of NuSAP1 reversed the inhibitory effects of ANKRD22 knockdown on the proliferation, invasion, and EMT of breast cancer cells. In summary, this study demonstrated that ANKRD22 enhanced breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression, which might shed light on new therapeutic approaches for breast cancer.

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<p>Nucleolar and Spindle Associated Protein 1 (NUSAP1) Promotes Bladder Cancer Progression Through the TGF-β Signaling Pathway</p>

Cited by 23 publications

References 28 publications

NUSAP1 Accelerates Osteosarcoma Cell Proliferation and Cell Cycle Progression via Upregulating CDC20 and Cyclin A2

NUSAP1 Accelerates Osteosarcoma Cell Proliferation and Cell Cycle Progression via Upregulating CDC20 and Cyclin A2

Four Novel Prognostic Genes Related to Prostate Cancer Identified Using Co-expression Structure Network Analysis

ANKRD22 enhances breast cancer cell malignancy by activating the Wnt/β-catenin pathway via modulating NuSAP1 expression

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