Nucleocapsid Assembly of Baculoviruses

Zhao, Shufen; He, Guanghui; Yang, Yiheng; Liang, Changhao

doi:10.3390/v11070595

Cited by 15 publications

(21 citation statements)

References 66 publications

(88 reference statements)

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“…In the phase skewness profiles shown in Figure 4D, all these phases can be observed, likely validating our hypothesis: first baculovirus infection cycle from 24 h (infection time) to 48 h; and second infection from 48 to 72 hours. The fact that baculovirus and AAV induce a different phase skewness profile (decrease for baculovirus and increase for AAV) may be due to their different shape (rod vs icosahedral, respectively), and the fact that baculovirus nucleocapsid is assembled in another nucleus region, the virogenic stroma [40], among other factors.…”

Section: Discussionmentioning

confidence: 99%

Holographic Imaging of Insect Cell Cultures: Online Non-Invasive Monitoring of Adeno-Associated Virus Production and Cell Concentration

et al. 2020

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The insect cell-baculovirus vector system has become one of the favorite platforms for the expression of viral vectors for vaccination and gene therapy purposes. As it is a lytic system, it is essential to balance maximum recombinant product expression with harvest time, minimizing product exposure to detrimental proteases. With this purpose, new bioprocess monitoring solutions are needed to accurately estimate culture progression. Herein, we used online digital holographic microscopy (DHM) to monitor bioreactor cultures of Sf9 insect cells. Batches of baculovirus-infected Sf9 cells producing recombinant adeno-associated virus (AAV) and non-infected cells were used to evaluate DHM prediction capabilities for viable cell concentration, culture viability and AAV titer. Over 30 cell-related optical attributes were quantified using DHM, followed by a forward stepwise regression to select the most significant (p < 0.05) parameters for each variable. We then applied multiple linear regression to obtain models which were able to predict culture variables with root mean squared errors (RMSE) of 7 × 105 cells/mL, 3% for cell viability and 2 × 103 AAV/cell for 3-fold cross-validation. Overall, this work shows that DHM can be implemented for online monitoring of Sf9 concentration and viability, also permitting to monitor product titer, namely AAV, or culture progression in lytic systems, making it a valuable tool to support the time of harvest decision and for the establishment of controlled feeding strategies.

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Section: Discussionmentioning

confidence: 99%

Holographic Imaging of Insect Cell Cultures: Online Non-Invasive Monitoring of Adeno-Associated Virus Production and Cell Concentration

et al. 2020

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“…Despite the genomic similarities between baculoviruses and nudiviruses, the differences in their replication strategies are significant (4,12). The large arsenal of genetic manipulation tools available for different species belonging to the Baculoviridae family has allowed to decipher the role of specific genes at diverse stages of their life cycle (10).…”

Section: Resultsmentioning

confidence: 99%

“…A total of 32 genes were found to be common for all nudiviruses, 21 of which are also shared with baculoviruses (11). Nevertheless, the relation between the two families proved to be more complicated as baculoviruses can assemble into a well-characterized nonoccluded form (12), while some nudiviruses could also form occlusion bodies during replication (11).…”

mentioning

confidence: 99%

Visualizing Nudivirus Assembly and Egress

Velamoor

Mitchell

Humbel

et al. 2020

mBio

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Enveloped viruses hijack cellular membranes in order to provide the necessary material for virion assembly. In particular, viruses that replicate and assemble inside the nucleus have developed special approaches to modify the nuclear landscape for their advantage. We used electron microscopy to investigate cellular changes occurring during nudivirus infection and we characterized a unique mechanism for assembly, packaging, and transport of new virions across the nuclear membrane and through the cytoplasm. Our three-dimensional reconstructions describe the complex remodeling of the nuclear membrane necessary to release vesicle-associated viruses into the cytoplasm. This is the first report of nuclear morphological reconfigurations that occur during nudiviral infection. IMPORTANCE The dynamics of nuclear envelope has a critical role in multiple cellular processes. However, little is known regarding the structural changes occurring inside the nucleus or at the inner and outer nuclear membranes. For viruses assembling inside the nucleus, remodeling of the intranuclear membrane plays an important role in the process of virion assembly. Here, we monitored the changes associated with viral infection in the case of nudiviruses. Our data revealed dramatic membrane remodeling inside the nuclear compartment during infection with Oryctes rhinoceros nudivirus, an important biocontrol agent against coconut rhinoceros beetle, a devastating pest for coconut and oil palm trees. Based on these findings, we propose a model for nudivirus assembly in which nuclear packaging occurs in fully enveloped virions.

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“…Newly formed nucleocapsids are assembled in the virogenic stroma (Figure 10.3) (Zhao et al, 2019) and are then transported to an electron-lucent region at the periphery of the nucleus, which has been named the "ring zone" (Figure 10.5). For multiple nucleopolyhedroviruses, nucleocapsids may also circumvent the nucleus and transit directly through the midgut epithelial cell to the basal membranes of those cells, where they bud into the hemocoel or tracheal cells (Granados and Lawler, 1981;Washburn et al, 1999) (Figure 10.4).…”

Section: Infection Cyclementioning

confidence: 99%

Genetic diversity in Chrysodeixis includens nucleopolyhedrovirus and factors involved in its generation

Aguirre¹

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In the present thesis, the genetic variability of field-collected isolates of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) was examined. These results have revealed the great variability present within natural ChinNPV isolates, confirm the differences between two different genetic variants from these isolates and, more important, appear to identify the ChinNPV-K variant as a generator of variability in these isolates. In conclusion, a great variability has been revealed within ChinNPV natural isolates, with a single variant showing a key role in the generation of this diversity in a concentration-dependent manner.

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Nucleocapsid Assembly of Baculoviruses

Cited by 15 publications

References 66 publications

Holographic Imaging of Insect Cell Cultures: Online Non-Invasive Monitoring of Adeno-Associated Virus Production and Cell Concentration

Holographic Imaging of Insect Cell Cultures: Online Non-Invasive Monitoring of Adeno-Associated Virus Production and Cell Concentration

Visualizing Nudivirus Assembly and Egress

Genetic diversity in Chrysodeixis includens nucleopolyhedrovirus and factors involved in its generation

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