Nucleic Acid Reassociation as a Guide to Genetic Relatedness among Bacteria

Genome comparison in yeast systematics: delimitation of species within the genera Schwanniomyces, Saccharomyces, Debaryomyces, and Pichia.

“…The Britten-Kohne plots (25) in Fig. 4A and 4C show that the reactions were essentially complete at Cot 60, with about 86 and 85% renaturation of DNA from S. rosei 74-32 and S. florentinus 66-13, respectively. No significant renaturation occurred during the last quarter of the reaction with either preparation.…”

“…With the exception of Ouchi et al (92), who used the agar support method (16), all yeast polynucleotide sequence comparisons have been assayed by the nitrocellulose filter technique (35,46). The problems inherent in this method are well known and are often magnified in eucaryotic systems (24,86). These problems include (i) relatively limited reannealing and (ii) leaching of DNA from the filters during the renaturation reaction.…”

“…Of these yeasts, all but D. subglobosus could reasonably comprise a single species, inasmuch as they shared at least 77% of their nucleotide sequences with the reference DNA from the type strain of D. hansenii. When the renaturation kinetics of D. hansenii [74][75][76][77][78][79][80][81][82][83][84][85][86] labeled reference DNA and heterologous unlabeled DNA were followed as a function of time, all reactions involving partially related heterologous DNAs were essentially complete as measured at Cot 80, with the possible exception of the mixture using DNA from the type strain of D. subglobosus. This DNA, with a base compposition 1.3% lower than that of the D. hansenii type, retained 37% rather poorly matched sequences in common with the reference DNA.…”

Identification and classification of Campylobacter strains by using nonradioactive DNA probes

“…Now that we are aware of the diversity of the genus Campylobacter, the exact identification of these bacteria has become crucial in defining the disease spectrum of each species as well as for epidemiological purposes. Nucleic acid hybridization is the reference method used in taxonomy (8,23). It has been applied to the identification of some Campylobacter species by using radiolabeled total DNA probes and dot blot hybridization techniques (9,11,26,34,39,42,44) and to the classification of some of them (14).…”

Genotypic relationships between strains ofAnabaena(Cyanophyceae) and their correlation with morphological affinities

“…The wide use of DNA-DNA hybridization in the taxonomy of prokaryotes was reviewed by Moore (1974) and Bradley (1980). Hybridizations with blue-green algal DNA were carried out by Stam& Venema (1977), Stam (1980), and Lachance (1981).…”