Nucleic Acid Integrated Technologies for Electrochemical Point‐of‐Care Diagnostics: A Comprehensive Review

Surucu, Ozge; Öztürk, Elif; Kuralay, Filiz

doi:10.1002/elan.202100309

Cited by 8 publications

(7 citation statements)

References 122 publications

(102 reference statements)

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“…The CV curve of CuNW grown on the surface of SPCE is similar to that of Cu/SPCE. These results indicate the formation of CuNW on SPCE [ 40 ]. In CuNW/SPCE, the peak at 0.3 V may be due to the formation of soluble material by CuNW and OH − .…”

Section: Resultsmentioning

confidence: 90%

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An Electrochemical-Based Point-of-Care Testing Methodology for Uric Acid Measurement

Zhao

Song

2022

Journal of Analytical Methods in Chemistry

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Point-of-care technology (POCT) is an important method in clinical testing in the future, which can achieve the purpose of rapid analysis. In this work, we assembled an electrochemical POC sensor for uric acid (UA) by surface modification of a screen-printed electrode. Copper nanowires were used as electrode modifiers to achieve high-performance electrochemical oxidation of UA. This electrochemical sensor can achieve linear detection of UA in the range of 10 μM to 2 mM. The detection limit of the sensor was calculated to be 2 μM. Although the detection performance of this sensor is not competitive with high-performance electrochemical sensors, it has been able to meet the needs of POC detection. At the same time, the sensor has excellent anti-interference performance. It has also been used successfully to test urine and serum samples from healthy and gout patients.

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Section: Resultsmentioning

confidence: 90%

“…This phenomenon indicates that CuNW indeed causes the electrocatalytic oxidation signal of UA. In addition, this indicates that the sensor has a high electrocatalytic activity for electrochemical oxidation of UA [ 40 – 42 ].…”

Section: Resultsmentioning

confidence: 99%

An Electrochemical-Based Point-of-Care Testing Methodology for Uric Acid Measurement

Zhao

Song

2022

Journal of Analytical Methods in Chemistry

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“…Electrochemical sensors have demonstrated promising benefits, such as rapid detection with high sensitivity and selectivity for developing POC diagnostic tools [ 62 , 63 ]. They have been extensively explored for the detection of single nucleotide polymorphisms (SNPs) [ 64 , 65 ], nucleic acids, and their amplified products over the past few decades [ 66 , 67 , 68 ].…”

Section: Lamp Detection Methodsmentioning

confidence: 99%

LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection

2022

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Seeking optimized infectious pathogen detection tools is of primary importance to lessen the spread of infections, allowing prompt medical attention for the infected. Among nucleic-acid-based sensing techniques, loop-mediated isothermal amplification is a promising method, as it provides rapid, sensitive, and specific detection of microbial and viral pathogens and has enormous potential to transform current point-of-care molecular diagnostics. In this review, the advances in LAMP-based point-of-care diagnostics assays developed during the past few years for rapid and sensitive detection of infectious pathogens are outlined. The numerous detection methods of LAMP-based biosensors are discussed in an end-point and real-time manner with ideal examples. We also summarize the trends in LAMP-on-a-chip modalities, such as classical microfluidic, paper-based, and digital LAMP, with their merits and limitations. Finally, we provide our opinion on the future improvement of on-chip LAMP methods. This review serves as an overview of recent breakthroughs in the LAMP approach and their potential for use in the diagnosis of existing and emerging diseases.

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“…Label-free electrochemical biosensors have a high capability of being adapted into point-of-care (POC) systems that can be used for outside the laboratory testing to minimize the need for healthcare services such as hospitals [ 14 , 143 , 144 , 145 ]. In POC testing particularly, microfluidic devices have attracted great attention lately for effective and accurate cancer diagnosis owing to their ability to separate analytes at a good resolution in a rapid reaction time and to minimize the handling errors and costs [ 143 ].…”

Section: Label-free Electrochemical Cancer Biosensors For Point-of-ca...mentioning

confidence: 99%

Label-Free Electrochemical Biosensor Platforms for Cancer Diagnosis: Recent Achievements and Challenges

Şanko

Kuralay

2023

Biosensors

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With its fatal effects, cancer is still one of the most important diseases of today’s world. The underlying fact behind this scenario is most probably due to its late diagnosis. That is why the necessity for the detection of different cancer types is obvious. Cancer studies including cancer diagnosis and therapy have been one of the most laborious tasks. Since its early detection significantly affects the following therapy steps, cancer diagnosis is very important. Despite researchers’ best efforts, the accurate and rapid diagnosis of cancer is still challenging and difficult to investigate. It is known that electrochemical techniques have been successfully adapted into the cancer diagnosis field. Electrochemical sensor platforms that are brought together with the excellent selectivity of biosensing elements, such as nucleic acids, aptamers or antibodies, have put forth very successful outputs. One of the remarkable achievements of these biomolecule-attached sensors is their lack of need for additional labeling steps, which bring extra burdens such as interference effects or demanding modification protocols. In this review, we aim to outline label-free cancer diagnosis platforms that use electrochemical methods to acquire signals. The classification of the sensing platforms is generally presented according to their recognition element, and the most recent achievements by using these attractive sensing substrates are described in detail. In addition, the current challenges are discussed.

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Nucleic Acid Integrated Technologies for Electrochemical Point‐of‐Care Diagnostics: A Comprehensive Review

Cited by 8 publications

References 122 publications

An Electrochemical-Based Point-of-Care Testing Methodology for Uric Acid Measurement

An Electrochemical-Based Point-of-Care Testing Methodology for Uric Acid Measurement

LAMP-Based Point-of-Care Biosensors for Rapid Pathogen Detection

Label-Free Electrochemical Biosensor Platforms for Cancer Diagnosis: Recent Achievements and Challenges

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