An Electrochemical-Based Point-of-Care Testing Methodology for Uric Acid Measurement

Zhao, Yuetong; Song, Xia

doi:10.1155/2022/8555842

Cited by 4 publications

(1 citation statement)

References 46 publications

(46 reference statements)

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“…Over the past two decades, many attempts have been made to adopt biosensor technology as a point-of-care testing (POC) tool [ 12 ]. Electrochemical biosensors are most suitable for POC technologies due to their fast response, portability, and low cost [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Different Foodborne Bacteria for Point-of-Care Applications

Yagati

Min

2023

Biosensors

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Bacterial infections resulting from foodborne pathogenic bacteria cause millions of infections that greatly threaten human health and are one of the leading causes of mortality around the world. To counter this, the early, rapid, and accurate detection of bacterial infections is very important to address serious health issue concerns. We, therefore, present an electrochemical biosensor based on aptamers that selectively bind with the DNA of specific bacteria for the accurate and rapid detection of various foodborne bacteria for the selective determination of bacterial infection types. Different aptamers were synthesized and immobilized on Au electrodes for selective bindings of different types of bacterial DNA (Escherichia coli, Salmonella enterica, and Staphylococcus aureus) for the accurate detection and quantification of bacterial concentrations from 101 to 107 CFU/mL without using any labeling methods. Under optimized conditions, the sensor showed a good response to the various concentrations of bacteria, and a robust calibration curve was obtained. The sensor could detect the bacterial concentration at meager quantities and possessed an LOD of 4.2 × 101, 6.1 × 101, and 4.4 × 101 CFU/mL for S. Typhimurium, E. Coli, and S. aureus, respectively, with a linear range from 100 to 104 CFU/mL for the total bacteria probe and 100 to 103 CFU/mL for individual probes, respectively. The proposed biosensor is simple and rapid and has shown a good response to bacterial DNA detections and thus can be applied in clinical applications and food safety monitoring.

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Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Different Foodborne Bacteria for Point-of-Care Applications

Yagati

Min

2023

Biosensors

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Quantitative analysis of nutrients for nucleosides, nucleobases, and amino acids hidden behind five distinct regions‐derived Poria cocos using ultra‐performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Zhao

Bian

Shan³

et al. 2022

J of Separation Science

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Poria cocos is an edible fungus used as a health product and traditional Chinese medicinal preparation. Nevertheless, little is known about its nutrients. In this study, ultra‐high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry was conducted to quantify nucleosides, nucleobases, and amino acids in 32 batches of Poria cocos samples collected from Anhui, Sichuan, Hubei, Hunan, and Guizhou. Subsequently, the linearity, precision, repeatability, stability, and recovery of our methods were validated. Samples from different regions were clearly separated by partial least squares discriminant analysis and cluster analysis. Our results suggested that Poria cocos samples from different geographical environments differed in nucleosides, nucleobases, and amino acids. The plot of variable importance for projection disclosed differential compositions of L‐Leucine, Uridine, L‐Asparagine, L‐Glutamine, L‐phenylalanine, L‐Ornithine monohydrochloride, L‐Hydroxyproline, Taurine, and Inosine in Poria cocos from five regions. We found the highest content of total analytes, total amino acids, and total non‐essential amino acids in Poria cocos from Anhui, total essential amino acids in the Sichuan samples, and total nucleosides in the Hunan samples. Overall, we determined the content of Poria cocos‐derived nucleosides, nucleobases, and amino acids, providing the foothold for further chemical mining and use of Poria cocos.

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Comparative analysis of infrared and electrochemical fingerprints of different medicinal parts of Eucommia ulmoides Oliver

Huang¹,

Zhang²,

Rao³

2023

International Journal of Electrochemical Science

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An Electrochemical-Based Point-of-Care Testing Methodology for Uric Acid Measurement

Cited by 4 publications

References 46 publications

Electrochemical Detection of Different Foodborne Bacteria for Point-of-Care Applications

Electrochemical Detection of Different Foodborne Bacteria for Point-of-Care Applications

Quantitative analysis of nutrients for nucleosides, nucleobases, and amino acids hidden behind five distinct regions‐derived Poria cocos using ultra‐performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Comparative analysis of infrared and electrochemical fingerprints of different medicinal parts of Eucommia ulmoides Oliver

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