Nucleic acid diagnostics on the total integrated lab-on-a-disc for point-of-care testing

Point-of-care diagnostics for infectious diseases: From methods to devices

“…LOAD device is a kind of centrifugal microfluidics, which integrates sample preparation, solution translocation, reagent mixing, separation and detection in a single device [57] , [58] . Compared with LOC devices, LOAD devices exhibit advantages of automatic fluid actuation without tubing, external pumping, actuators or active valves, a high degree of parallelization, and a huge capacity for total integration [361] , [362] , [363] , [364] , [365] , [366] , [367] .…”

“…Compared with LOC devices, LOAD devices exhibit advantages of automatic fluid actuation without tubing, external pumping, actuators or active valves, a high degree of parallelization, and a huge capacity for total integration [361] , [362] , [363] , [364] , [365] , [366] , [367] . In a LOAD device, the movement of the solution is controlled by the synthetic action of centrifugal forces, capillary forces, Euler forces and Coriolis forces, which can be easily manipulated by modulating the spinning speed, spinning direction, valves or rotation actuated barriers, making the device be able to work smoothly and efficiently with a simple spindle motor and a sophisticated microfluidic design [57] , [58] , [367] , [368] , [369] , [370] . In the past years, LOAD devices have experienced great prosperity and development, which have been regarded as one of the most promising alternatives for the development of automated POC devices.…”

“…Moreover, empowered by the booming development of microfluidics, micro electro-mechanical systems (MEMS) technology, nanotechnology and materials science, all flowers are in bloom in the development of POC devices. Those POC devices including lab-on-a-chip (LOC) devices [54] , [55] , [56] , lab-on-a-disc (LOAD) devices [57] , [58] , microfluidic paper-based analytical devices ( μ PADs) [59] , lateral flow devices [60] , miniaturized PCR devices [61] , and isothermal nucleic acid amplification devices (INAA) devices [62] , [63] , which substantially promoted the development of POC diagnostics. In particular, the seamless integration of those novel biosensors, advanced devices and recent artificial intelligence (AI) techniques would greatly accelerate the pace of progress in the development POC diagnostics toward labor-free and intelligent diagnosis.…”

Point-of-Care Strategies Applied to Malaria Diagnosis

“…Among the microfluidic devices, the centrifugal microfluidic device or lab-on-adisc (LOAD) has advanced remarkably due to simple operation by rotation, allowing for total integration of protocol steps, and high-throughput capability. LOAD devices have been extensively used for molecular diagnostic assays [58]. Among many targets and applications, one stands out relative to our goals in this text.…”

Point-of-care DNA testing by automatically and sequentially performing extraction, amplification and identification in a closed-type cassette

“…As the naked eye is a good enough sensor, colorimetric detection is an instrument-free type of detection, which is very suitable for POCT [ [23] , [24] , [25] ]. Lateral flow strip is a cheap and simple way for colorimetrically detecting amplicons, and has been used for POCT in no-airtight devices [ 26 , 27 ].…”