Nucleic Acid Content Changes of a Tropical Freshwater Fish Labeo rohita Fed Gelatinized and Nongelatinized Starch Diet

Abstract

A 60‐d feeding trial was conducted to study the effect of dietary gelatinized (G) and nongelatinized (NG) starch on deposition of nucleic acid in liver and muscle of Labeo rohita juveniles. Two hundred and thirty‐four juveniles (av. wt. 2.53 ± 0.04 g) were randomly distributed into six triplicate experimental groups. Six semipurified diets containing graded levels of (0, 20, 40, 60, 80, 100) NG and G corn starch, were prepared accordingly: T1 (100% NG, 0% G); T2 (80% NG, 20% G); T3 (60% NG, 40% G); T4 (40% NG, 60% G); T5 (20% NG, 80% G); and T6 (0% NG, 100% G). After a feeding period of 60 d, DNA content in muscle and liver decreased linearly with increasing level of G starch in the diet, whereas protein/DNA in muscle increased linearly with increasing levels of G starch in the diet. No significant effect of G starch on RNA deposition in liver and muscle was found. RNA/DNA ratio increased linearly with increasing level of gelatinized starch in the diet. Results from this study indicate that protein/DNA and RNA/DNA ratio in muscle increase with increasing levels of G starch in the diet, which also reflects in terms of growth performance.

Effects of different suboptimal dietary protein levels on growth, nutrient utilization and physio‐metabolic status of Anabas testudineus fingerlings in inland saline water

“…The RNA concentration and RNA/DNA ratios were corroborating to the good growth observed in T32 or T34 group. Similar results of positive relation of RNA/DNA ratio with growth performance was also observed in Labeo rohita (Gangadhar et al, 1997; Kumar et al, 2010). Therefore, results indicate that further increase in protein level is required to achieve maximum growth potential of fish A .…”

“…However, subsequently on long‐term exposure the values return to normal by compensatory influx of water as a result of re‐establishment of the extracellular volume (Martínez‐Álvarez et al, 2005; Shahkar et al, 2015). Total protein constitutes of nutritive component that play important role in immune functions (Kumar et al, 2010). In the present study, higher value of serum total protein in the higher protein fed groups suggests the dietary composition or the environmental condition does not influence the immune potential of A .…”

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“…This study also signifies the common assumption that the protein synthesis is a function of RNA synthesis in the respective muscles. Recently Kumar et al (2010) also reported that dietary supplementation of gelatinized and non-gelatinized starch has changed RNA content of the different tissues of the L. Rohita due to variable fraction of crude protein in the two starch diets. Study also revealed that the gelatinized starch could increase the muscle protein contents as compared to nongelatinized starch which might be attributed to elevated RNA content of the respective muscles.…”

Growth performance and chemical composition of Cirrhinus mrigala (mori) under the effect of chromium chloride hexahydratre

“…All dry ingredients weighted carefully, ground and mixed thoroughly and add sufficient amount of water (50 ml) to make it dough mix this mixture for half an hour. Spread this mixture in tray then placed in oven for drying purpose [13]. This material was grounded to make pellets (2 mm).…”