Nucleic acid-based therapeutics for dermal wound healing

Sharma, Priyank; Kumar, Arun; Agarwal, Tarun; Dey, Asmita Deka; Moghaddam, Farnaz Dabbagh; Rahimmanesh, Illnaz; Ghovvati, Mahsa; Yousefiasl, Satar; Borzacchiello, Assunta; Mohammadi, Abbas; Yella, Venkata Rajesh; Moradi, Omid; Sharifi, Esmaeel

doi:10.1016/j.ijbiomac.2022.08.099

Cited by 14 publications

(12 citation statements)

References 142 publications

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“…41 Also, future studies can explore the improvement of miRNA-related delivery methods, such as loading miRNA-related drugs into microneedles or hydrogels for delivery, which may further improve the efficacy. [42][43][44]…”

Section: Dovepressmentioning

confidence: 99%

MicroRNA-182-5p Inhibits Hypertrophic Scar Formation by Inhibiting the Proliferation and Migration of Fibroblasts via SMAD4 Pathway

Jin¹,

Xu²

2023

CCID

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Secondary to war wounds, trauma, etc., hypertrophic scar formation is the cause of an excessive proliferation of fibroblasts and accumulation of collagen fibers, which might affect cosmetic appearance, and could cause malignant transformation. miRNAs play an important role in disease regulation via inhibiting post-transcriptional protein translation by targeting and binding to the 3' UTR region of mRNA. Here we explore the mechanism and interventions of scar formation from the perspective of miRNA. Methods: Hypertrophic scar-associated differential miRNAs were screened by analyzing sequencing data of normal skin and hypertrophic scar, and verified by RT-qPCR. Signaling pathways that may be influenced by differentially miRNAs were analyzed using KEGG and GO. miRNA mimics were used to explore the effects of miRNAs on SMAD signaling pathway proteins. Dualluciferase assays were used to explore the targeted binding of miRNAs. The mimics of the miRNA were used to explore the impact of miRNAs on the proliferation, migration, apoptosis and collagen synthesis levels of hypertrophic scar fibroblasts. The scar model of rabbit ear was used to verify the influence of miRNA on wound healing and scar formation in vivo. Results: Expression of miR-182-5p was found to be considerably decreased in hypertrophic scars and fibroblasts. miR-182-5p was found to act mainly by targeting the 3'UTR region of SMAD4, but not SMAD1 or SMAD3. miR-182-5p overexpression may drastically suppress the proliferation and migration of fibroblasts, accompanied by enhanced apoptosis and reduced collagen fiber synthesis. The overexpression of miR-182-5p in in vivo experiments could effectively inhibit hypertrophic scar formation without affecting the speed and quality of wound healing. Conclusion: miR-182-5p inhibits hypertrophic scar formation by decreasing the proliferation and migration of fibroblasts via SMAD4 pathway, and is expected to become a novel hypertrophic scar therapeutic target.

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Section: Dovepressmentioning

confidence: 99%

MicroRNA-182-5p Inhibits Hypertrophic Scar Formation by Inhibiting the Proliferation and Migration of Fibroblasts via SMAD4 Pathway

Jin¹,

Xu²

2023

CCID

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“…283 Given that macrophages are activated and amplified in RA, Jain et al 285 studied the effectiveness of nonviral gene transfection strategies to repolarize macrophages from M1 to M2 phenotypes to treat RA. Anti-inflammatory cytokines, such as IL-10, encoding plasmid DNA, were successfully 284,[286][287][288][289] encapsulated by noncondensed alginate-based nanoparticles, the surfaces of which were modified with prophagocytic peptides to achieve active macrophage targeting. Therapy significantly reduced the expression of the proinflammatory cytokines TNF-α, IL-1β, and IL-6 in systemic and joint tissues and arrested the progression of inflammation and joint damage.…”

Section: Rheumatoid Arthritismentioning

confidence: 99%

Biomaterial‐based gene therapy

Gao

et al. 2023

MedComm

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Gene therapy, a medical approach that involves the correction or replacement of defective and abnormal genes, plays an essential role in the treatment of complex and refractory diseases, such as hereditary diseases, cancer, and rheumatic immune diseases. Nucleic acids alone do not easily enter the target cells due to their easy degradation in vivo and the structure of the target cell membranes. The introduction of genes into biological cells is often dependent on gene delivery vectors, such as adenoviral vectors, which are commonly used in gene therapy. However, traditional viral vectors have strong immunogenicity while also presenting a potential infection risk. Recently, biomaterials have attracted attention for use as efficient gene delivery vehicles, because they can avoid the drawbacks associated with viral vectors. Biomaterials can improve the biological stability of nucleic acids and the efficiency of intracellular gene delivery. This review is focused on biomaterial‐based delivery systems in gene therapy and disease treatment. Herein, we review the recent developments and modalities of gene therapy. Additionally, we discuss nucleic acid delivery strategies, with a focus on biomaterial‐based gene delivery systems. Furthermore, the current applications of biomaterial‐based gene therapy are summarized.

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“…In recent years, with the increasing understanding of the wound healing process, nucleic acid therapies that enhance or inhibit different signaling pathways at the genetic level have become a new idea in wound therapy with the advantages of “longer duration of action, higher specificity of action, higher target selectivity, and software-designable sequences” [ 84 , 85 ]. Gene therapy for wounds mainly refers to therapeutic nucleic acids (including small interfering RNA (siRNA), oligonucleotides, plasmid DNA, antisense oligonucleotides (ASO), microRNA (miRNA) mimics, anti-miRNA oligonucleotide (AMO), aptamers, and messenger RNA (mRNA)) that regulate cell motility, angiogenesis, epithelialization, and oxidative stress through different signaling pathways in various steps of wound healing to achieve a more efficient promotion of wound healing [ 86 , 87 , 88 ].…”

Section: Hydrogel Dressing Loaded With Nanomedicinesmentioning

confidence: 99%

Current Progress and Outlook of Nano-Based Hydrogel Dressings for Wound Healing

et al. 2022

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Wound dressing is an important tool for wound management. Designing wound dressings by combining various novel materials and drugs to optimize the peri-wound environment and promote wound healing is a novel concept. Hydrogels feature good ductility, high water content, and favorable oxygen transport, which makes them become some of the most promising materials for wound dressings. In addition, nanomaterials exhibit superior biodegradability, biocompatibility, and colloidal stability in wound healing and can play a role in promoting healing through their nanoscale properties or as carriers of other drugs. By combining the advantages of both technologies, several outstanding and efficient wound dressings have been developed. In this paper, we classify nano-based hydrogel dressings into four categories: hydrogel dressings loaded with a nanoantibacterial drug; hydrogel dressings loaded with oxygen-delivering nanomedicines; hydrogel dressings loaded with nanonucleic acid drugs; and hydrogel dressings loaded with other nanodelivered drugs. The design ideas, advantages, and challenges of these nano-based hydrogel wound dressings are reviewed and analyzed. Finally, we envisaged possible future directions for wound dressings in the context of relevant scientific and technological advances, which we hope will inform further research in wound management.

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Nucleic acid-based therapeutics for dermal wound healing

Cited by 14 publications

References 142 publications

MicroRNA-182-5p Inhibits Hypertrophic Scar Formation by Inhibiting the Proliferation and Migration of Fibroblasts via SMAD4 Pathway

MicroRNA-182-5p Inhibits Hypertrophic Scar Formation by Inhibiting the Proliferation and Migration of Fibroblasts via SMAD4 Pathway

Biomaterial‐based gene therapy

Current Progress and Outlook of Nano-Based Hydrogel Dressings for Wound Healing

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