Nucleic acid based polymer and nanoparticle conjugates: Synthesis, properties and applications

Kundu, Aniruddha; Nandi, S.; Nandi, Arun K.

doi:10.1016/j.pmatsci.2017.04.001

Cited by 25 publications

(14 citation statements)

References 376 publications

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“…polymer micelles play a vital role in cancer nanotechnology and in novel drug delivery system to deliver drug at specific site [103]. Poly (ethylene imine) PEI is most widely used polymer in nucleic acid based nanostructure drug delivery [104].…”

Section: Nucleic Acids Based Nanostructuresmentioning

confidence: 99%

Recent Advances in Self-Assembled Nanoparticles for Drug Delivery

Varma

Singh

Gorain

et al. 2020

CDD

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The collection of different bulk materials forms the nanoparticles, where the properties of the nanoparticle are solely different from the individual components before being ensembled. Selfassembled nanoparticles are basically a group of complex functional units that are formed by gathering the individual bulk components of the system. It includes micelles, polymeric nanoparticle, carbon nanotubes, liposomes and niosomes, <i>etc</i>. This self-assembly has progressively heightened interest to control the final complex structure of the nanoparticle and its associated properties. The main challenge of formulating self-assembled nanoparticle is to improve the delivery system, bioavailability, enhance circulation time, confer molecular targeting, controlled release, protection of the incorporated drug from external environment and also serve as nanocarriers for macromolecules. Ultimately, these self-assembled nanoparticles facilitate to overcome the physiological barriers <i>in vivo</i>. Self-assembly is an equilibrium process where both individual and assembled components are subsisting in equilibrium. It is a bottom up approach in which molecules are assembled spontaneously, non-covalently into a stable and welldefined structure. There are different approaches that have been adopted in fabrication of self-assembled nanoparticles by the researchers. The current review is enriched with strategies for nanoparticle selfassembly, associated properties, and its application in therapy.

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Section: Nucleic Acids Based Nanostructuresmentioning

confidence: 99%

Recent Advances in Self-Assembled Nanoparticles for Drug Delivery

Varma

Singh

Gorain

et al. 2020

CDD

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“…T he development and application of DNA-nanoparticle conjugates for advanced chemical and biological sensing is a continuously growing field of research. − Understanding conformation and function of nucleic acids attached to nanosurfaces under physiological conditions is of paramount importance for the design of useful and versatile analytical tools with specific and sensitive target recognition. Semiconductor quantum dots (QDs) are among the most often applied nanoparticles for DNA-nanobiotechnology because their chemical and photophysical stability and outstanding fluorescence properties can be used for both investigating the DNA-nanosurface interaction and developing robust and sensitive biosensors. − In particular, the combination with Förster resonance energy transfer (FRET) or other nanometric energy or charge-transfer mechanisms allows for scrutinizing distance dependence and orientation of DNA-nanoparticle systems and for developing and optimizing DNA-hybridization biosensors with exceptional analytical properties. − …”

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confidence: 99%

Conformational Details of Quantum Dot-DNA Resolved by Förster Resonance Energy Transfer Lifetime Nanoruler

et al. 2018

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DNA-nanoparticle conjugates are important tools in nanobiotechnology. Knowing the orientation, function, and length of DNA on nanoparticle surfaces at low nanomolar concentrations under physiological conditions is therefore of great interest. Here, we investigate the conformation of a 31 nucleotides (nt) long DNA attached to a semiconductor quantum dot (QD) via Forster resonance energy transfer (FRET) from Tb-DNA probes hybridized to different positions on the QD-DNA. Precise Tb-to-QD distance determination from 7 to 14 nm along 26 nt of the peptide-appended QD-DNA was realized by time-resolved FRET spectroscopy. The FRET nanoruler measured linear single-stranded (ssDNA) and double-stranded (dsDNA) extensions of ∼0.15 and ∼0.31 nm per base, reflecting the different conformations. Comparison with biomolecular modeling confirmed the denser conformation of ssDNA and a possibly more flexible orientation on the QD surface, whereas the dsDNA was fully extended with radial orientation. The temporally distinct photoluminescence decays of the different DNA-FRET configurations were used for prototypical DNA hybridization assays that demonstrated the large potential for extended temporal multiplexing. The extensive experimental and theoretical analysis of 11 different distances/configurations of the same QD-DNA conjugate provided important information on DNA conformation on nanoparticle surfaces and will be an important benchmark for the development and optimization of DNA-nanobiosensors.

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“…Moreover, the polymeric carriers have been further engineered to respond to various endogenous and exogenous stimuli; the release of therapeutic oligonucleotides was highly localized to disease tissues, and the endosomal escape of the large oligonucleotides was significantly promoted, thereby increasing therapeutic efficacy and efficiency dramatically with reduced side effects. Currently, several clinical trials are ongoing based on the systematic combination of oligonucleotides and polymers, 190 and the medical uses of the synergistic combination (e.g., immunotherapy) are also expanding. 191 Even though oligonucleotides and polymers have complemented each other to actualize highly effective and efficient oligonucleotide therapy, several issues still remain.…”

Section: Discussionmentioning

confidence: 99%