Nucleic Acid Amplification Tests for Diagnosis of
            <i>Bordetella</i>
            Infections

Riffelmann, Marion; König, Carl Heinz Wirsing von; Caro, Valérie; Guiso, Nicole

doi:10.1128/jcm.43.10.4925-4929.2005

Cited by 156 publications

(128 citation statements)

References 21 publications

Supporting

Mentioning

123

Contrasting

Unclassified

Order By: Relevance

“…B. pertussis strain FDA 460 (Fim2+/Fim3+) and sera containing C10C2D5 monoclonal anti-Fim3 and F2β2G8 antiFim2) antibodies were used as references. Susceptibility to ampicillin (10 µg), erythromycin (15 µg Detection of IS481was carried out using an inhouse reference real-time PCR method as previously described [10] until August 2012. After that time, validated commercial kits were used for the detection of both IS481 and IS1001, i.e., Simplexa Bordetella pertussis/parapertussis (FOCUS Diagnostics Cypress, California, USA) until March 2013 and Bordetella pertussis/parapertussis triplex (Bio-Evolution, Brysur-Marne, France) until September 2013, following the manufacturer's protocols [11].…”

Section: Identification Serotyping and Susceptibility Testing Of Ismentioning

confidence: 99%

Pertussis in north-central and northwestern regions of Algeria

Benamrouche

Maamar

Lazri

et al. 2016

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Pertussis outbreaks continue to occur in many countries despite high vaccination coverage. Under-diagnosed cases in adolescents and adults may result in increased transmission to infants, who are at risk of severe pertussis. Additional measures to protect both groups should be considered. Methodology: Nasopharyngeal samples and sera were collected from patients and household contacts with clinically suspected pertussis. Diagnoses were confirmed by culture, real-time polymerase chain reaction (PCR), and serology. Bordetella pertussis isolates were characterized by antimicrobial sensitivity and fimbrial serotyping. Results: Of 392 participants, 134/248 patients (54%) and 66/144 contacts (45.8%) had confirmed pertussis infections. B. parapertussis was not detected. All B. pertussis isolates were sensitive to the antibiotics tested, and all expressed the Fim3, not the Fim2, fimbrial serotype. Most patients (81.2%) were <6 months (51.8% of whom were <3 months) of age; 77.6% were unvaccinated, and most positive contacts were mothers 20-40 years of age. Conclusions: Despite high vaccination coverage, pertussis is circulating in Algeria. Most infections occur in unvaccinated infants <6 months of age, with mothers as the main source of infection. An adolescent/adult booster should be considered. Adoption of sensitive and specific laboratory tests would improve pertussis diagnosis and surveillance.

show abstract

Section: Identification Serotyping and Susceptibility Testing Of Ismentioning

confidence: 99%

Pertussis in north-central and northwestern regions of Algeria

Benamrouche

Maamar

Lazri

et al. 2016

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…The other study, from the United States, reported that protection from pertussis after a fifth dose of DTaP among children who had received only DTaP vaccines was relatively short-lived and waned substantially each year and risk of pertussis increase by an average of 42% a year during the first five years 16 . A recent cohort study, from United Kingdom, suggested that the risk of pertussis was more than threefold higher in children who had received the preschool pertussis booster vaccination seven years or more previously, 59% of whom received three doses of whole cell pertussis vaccine 17 .…”

Section: Discussionmentioning

confidence: 99%

High frequency of pertussis in older children and adolescents with prolonged cough in Turkey

et al. 2016

View full text Add to dashboard Cite

Nasopharyngeal specimens were collected from 7-18 year old children, presenting with prolonged cough of two to four weeks' duration. Specimens were examined for B. pertussis by PCR.Of 101 children with prolonged cough, 20 (19.8%) had a positive PCR testing for B. pertussis. Children who were vaccinated ≥5 years previously had a 6.13-fold higher risk of PCR-confirmed pertussis than those who were vaccinated <5 years before. The classic symptoms of pertussis (paroxysmal cough, whooping and post-tussive vomiting) were seen in 30%, 15% and 25% of the patients with positive PCR, respectively; 55% of them had only a prolonged cough without any classic symptoms. Pertussis is common among Turkish children with prolonged cough, even after implementation of a fifth dose of pertussis vaccination and despite high vaccination coverage.

show abstract

“…Compared with the culture method, nucleic acid amplification tests such as the IS481-based real-time polymerase chain reaction (PCR) and B. pertussis-specific loop-mediated isothermal amplification (LAMP) are highly sensitive for the detection of B. pertussis DNA (17)(18)(19). Therefore, these nucleic acid amplification tests have been recommended for the accurate diagnosis of pertussis (20).…”

Section: Introductionmentioning

confidence: 99%

Concentrations of immunoglobulin G antibodies against pertussis toxin does not decrease over a long period of time in Japan

Sakakibara

Ohtani²,

Jinta

et al. 2015

10.1 Respiratory Infections

View full text Add to dashboard Cite

Objective Adult patients with pertussis rarely show typical symptoms, such as paroxysmal coughing, inspiratory "whoop", or post-tussive vomiting. While a culture is regarded as the gold standard for diagnosis, the sensitivity is very low. Therefore, the diagnosis of pertussis in adults in clinical practice is mostly based on single-sample serology using an enzyme-linked immunosorbent assay (ELISA) with the pertussis toxin antigen. Various cut-off values for the anti-pertussis toxin IgG (PT-IgG) have been proposed. It has been reported that concentrations of PT-IgG fall below the defined cut-off about 4.5 months after infection on average, and within 1 year in most patients. We investigated the distribution and time course of the PT-IgG levels. Methods The data were collected from the medical records. Patients The study retrospectively identified subjects who had visited Ikebukuro Otani Clinic, which is a specialized clinic for patients with cough. We retrospectively reviewed 406 patients with PT-IgG measurements to investigate the age distribution of PT-IgG levels. The changes in PT-IgG levels over time were assessed in the 205 patients who had more than one PT-IgG measurement. Results PT-IgG levels were ! 100 EU/mL in more than 15% of subjects. The PT-IgG levels of a few subjects had diminished over a long period of time. Conclusion A PT-IgG level greater than the defined cut-off value simply indicates past infection or immunization in most subjects. As such, a single measurement of PT-IgG using the cut-off values might lead to overdiagnosis of pertussis. Further data collection and analysis are required.

show abstract

Nucleic Acid Amplification Tests for Diagnosis of Bordetella Infections

Cited by 156 publications

References 21 publications

Pertussis in north-central and northwestern regions of Algeria

Pertussis in north-central and northwestern regions of Algeria

High frequency of pertussis in older children and adolescents with prolonged cough in Turkey

Concentrations of immunoglobulin G antibodies against pertussis toxin does not decrease over a long period of time in Japan

Contact Info

Product

Resources

About