Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute the<i>Drosophila</i>Mushroom Bodies

Shih, Min-Yi; Davis, Fred P.; Henry, Gilbert L.; Dubnau, Josh

doi:10.1534/g3.118.200726

Cited by 51 publications

(44 citation statements)

References 115 publications

(177 reference statements)

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“…6C and SI Appendix, Fig. S16); this result is consistent with the relative absence of histamine and histamine receptors in the mushroom body (54)(55)(56)(57)(58)(59)(60), and argues against nonspecific effects of histamine.…”

Section: Adaptation To Excess Inhibition From Apl Is Most Prominent Asupporting

confidence: 76%

Mechanisms underlying homeostatic plasticity in the Drosophila mushroom body in vivo

Apostolopoulou

Lin

2020

Proc. Natl. Acad. Sci. U.S.A.

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Neural network function requires an appropriate balance of excitation and inhibition to be maintained by homeostatic plasticity. However, little is known about homeostatic mechanisms in the intact central brain in vivo. Here, we study homeostatic plasticity in theDrosophilamushroom body, where Kenyon cells receive feedforward excitation from olfactory projection neurons and feedback inhibition from the anterior paired lateral neuron (APL). We show that prolonged (4-d) artificial activation of the inhibitory APL causes increased Kenyon cell odor responses after the artificial inhibition is removed, suggesting that the mushroom body compensates for excess inhibition. In contrast, there is little compensation for lack of inhibition (blockade of APL). The compensation occurs through a combination of increased excitation of Kenyon cells and decreased activation of APL, with differing relative contributions for different Kenyon cell subtypes. Our findings establish the fly mushroom body as a model for homeostatic plasticity in vivo.

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Section: Adaptation To Excess Inhibition From Apl Is Most Prominent Asupporting

confidence: 76%

Mechanisms underlying homeostatic plasticity in the Drosophila mushroom body in vivo

Apostolopoulou

Lin

2020

Proc. Natl. Acad. Sci. U.S.A.

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“…The complex interplay between genes, neurons and behavior started to be deciphered decades ago with the Benzerian revolution in neurogenetics and is still under intense investigation these days using a plethora of tools in various model organisms. This study joins a growing body of studies that use contemporary genomic approaches to dissect the brain into units and illuminate their molecular content, as a step toward understanding the dynamic spatiotemporal environments in which genes function (Croset et al, 2018;Agrawal et al, 2019;Shih et al, 2019). While many cell types exist in the fly brain, in this study, we analyzed only a small fraction of them, focusing mostly on NPFR neurons.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of NPFR Neurons Reveals a Connection Between Proteome Diversity and Social Behavior

Ryvkin

Bentzur

Shmueli

et al. 2021

Front. Behav. Neurosci.

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Social behaviors are mediated by the activity of highly complex neuronal networks, the function of which is shaped by their transcriptomic and proteomic content. Contemporary advances in neurogenetics, genomics, and tools for automated behavior analysis make it possible to functionally connect the transcriptome profile of candidate neurons to their role in regulating behavior. In this study we used Drosophila melanogaster to explore the molecular signature of neurons expressing receptor for neuropeptide F (NPF), the fly homolog of neuropeptide Y (NPY). By comparing the transcription profile of NPFR neurons to those of nine other populations of neurons, we discovered that NPFR neurons exhibit a unique transcriptome, enriched with receptors for various neuropeptides and neuromodulators, as well as with genes known to regulate behavioral processes, such as learning and memory. By manipulating RNA editing and protein ubiquitination programs specifically in NPFR neurons, we demonstrate that the proper expression of their unique transcriptome and proteome is required to suppress male courtship and certain features of social group interaction. Our results highlight the importance of transcriptome and proteome diversity in the regulation of complex behaviors and pave the path for future dissection of the spatiotemporal regulation of genes within highly complex tissues, such as the brain.

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“…Also, cholinergic activity in these cells impacts activity of MB output neurons (MBONs) (Barnstedt et al, 2016). Kenyon cells exclusively use ACh for intercellular communication, supporting ACh's excitatory role in the fly CNS (Shih et al, 2019).…”

Section: Acetylcholinementioning

confidence: 92%

The Neurotransmitters Involved in Drosophila Alcohol-Induced Behaviors

Chvilicek

Titos

Rothenfluh

2020

Front. Behav. Neurosci.

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Alcohol is a widely used and abused substance with numerous negative consequences for human health and safety. Historically, alcohol's widespread, non-specific neurobiological effects have made it a challenge to study in humans. Therefore, model organisms are a critical tool for unraveling the mechanisms of alcohol action and subsequent effects on behavior. Drosophila melanogaster is genetically tractable and displays a vast behavioral repertoire, making it a particularly good candidate for examining the neurobiology of alcohol responses. In addition to being experimentally amenable, Drosophila have high face and mechanistic validity: their alcohol-related behaviors are remarkably consistent with humans and other mammalian species, and they share numerous conserved neurotransmitters and signaling pathways. Flies have a long history in alcohol research, which has been enhanced in recent years by the development of tools that allow for manipulating individual Drosophila neurotransmitters. Through advancements such as the GAL4/UAS system and CRISPR/Cas9 mutagenesis, investigation of specific neurotransmitters in small subsets of neurons has become ever more achievable. In this review, we describe recent progress in understanding the contribution of seven neurotransmitters to fly behavior, focusing on their roles in alcohol response: dopamine, octopamine, tyramine, serotonin, glutamate, GABA, and acetylcholine. We chose these small-molecule neurotransmitters due to their conservation in mammals and their importance for behavior. While neurotransmitters like dopamine and octopamine have received significant research emphasis regarding their contributions to behavior, others, like glutamate, GABA, and acetylcholine, remain relatively unexplored. Here, we summarize recent genetic and behavioral findings concerning these seven neurotransmitters and their roles in the behavioral response to alcohol, highlighting the fitness of the fly as a model for human alcohol use.

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Nuclear Transcriptomes of the Seven Neuronal Cell Types That Constitute theDrosophilaMushroom Bodies

Cited by 51 publications

References 115 publications

Mechanisms underlying homeostatic plasticity in the Drosophila mushroom body in vivo

Mechanisms underlying homeostatic plasticity in the Drosophila mushroom body in vivo

Transcriptome Analysis of NPFR Neurons Reveals a Connection Between Proteome Diversity and Social Behavior

The Neurotransmitters Involved in Drosophila Alcohol-Induced Behaviors

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