Nuclear survivin expression as a potentially useful tool for the diagnosis of canine cutaneous sebaceous lesions

Bongiovanni, Laura; Suter, Maja M.; Malatesta, Daniela; Ordinelli, Alessandra; Ciccarelli, Andrea; Romanucci, Mariarita; Brenner, Ori; Salda, Leonardo Della

doi:10.1111/j.1365-3164.2012.01065.x

Cited by 12 publications

(17 citation statements)

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“…Bongiovanni et al () have determined that the immunolabeling of survivin and Ki67 is overlapped in canine epidermis, hair follicles and sebaceous glands (Bongiovanni et al, ). Recently, Bongiovanni et al () have found that survivin is expressed only in scattered reserve cells of normal sebaceous glands, where stem cells are supposed to reside. These authors have hypothesized that survivin could represent a marker for “sebaceous” stem cells or progenitor cells and they have suggested that survivin could possibly play a similar prosurvival role in sebocytes as in keratinocytes, guaranteeing the long life of stem cells in their niche (Bongiovanni et al, ).…”

Section: Discussionmentioning

confidence: 99%

The Process of Apoptosis in a Holocrine Gland as Shown by the Avian Uropygial Gland

Lïman

Alan

2013

The Anatomical Record

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This study was designed to elucidate the presence of apoptosis and the localization of apoptosis-related Bax and survivin proteins and proliferating cell nuclear antigen (PCNA) within the chicken uropygial gland, a specialized holocrine secretory gland. In day-old chicks, survivin and Bax immunoreactivities were observed in the cell cytoplasm of the germinative and secretory layers of the luminal epithelium and tubules. During this period, the TUNEL reaction, an indication of apoptosis, was only sporadically positive in the tubules. From the 7th day to the 150th day of posthatching, survivin was detected in the cytoplasm of cells in the germinative layer and in the nuclei of some cells in the secretory layers of the gland. The germinative layer cells showed weak homogeneous cytoplasmic staining for Bax, whereas the cells of the secretory and intermediate layers of luminal epithelium and tubules exhibited granular cytoplasmic staining. After day 7, TUNEL-positive cells were observed in the secretory and degenerative layers of the luminal epithelium and central tubules. After day 12, some TUNEL-positive cells were also seen in the peripheral tubules. At all posthatch ages, the cytoplasm and nucleus of the germinative layers of luminal epithelium and tubules reacted with PCNA, whereas only a small number of cell nuclei in the secretory layers were immunopositive. These results support the theory that specific PCNA/Bax/survivin expression patterns could reflect particular cell differentiation states in the uropygial gland and that holocrine secretion in the gland is realized mainly by way of apoptosis.

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Section: Discussionmentioning

confidence: 99%

The Process of Apoptosis in a Holocrine Gland as Shown by the Avian Uropygial Gland

Lïman

Alan

2013

The Anatomical Record

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“…MI and PI assessed as the number of mitotic figures and the percentage of Ki67-positive cells, respectively. According to our knowledge only a few such studies have been previously conducted on canine osteosarcomas (Bongiovanni et al 2012a, Davies et al 2012, Shoeneman et al 2012) and sebaceous lesions (Bongiovanni et al 2012b) and has not been previously reported in canine NHLs.…”

Section: Discussionmentioning

confidence: 99%

“…Immunohistochemical studies have revealed expression of this protein in some canine tumors including transitional cell carcinomas of urinary bladder (Rankin et al 2008), cutaneous tumors (Bongiovanni et al 2009(Bongiovanni et al , 2012b and osteosarcomas (Bongiovanni et al 2012a, Davies et al 2012, Shoeneman et al 2012.…”

Section: Introductionmentioning

confidence: 99%

Survivin expression in canine lymphomas in relation with proliferative markers

Sokołowska¹,

Urbańska²,

Gizinski³

et al. 2015

Polish Journal of Veterinary Sciences

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Survivin is a member of apoptosis inhibiting proteins family. Apart from its antiapoptotic activity it plays a critical role in regulating the cell cycle and mitosis. It is overexpressed in most human malignancies. While the prognostic significance of survivin expression is widely investigated in human non-Hodgkin's lymphomas, little is known about its expression in canine lymphomas. The aim of the study was to evaluate the expression of survivin in canine lymphomas in relation to proliferation markers (mitotic index and percentage of Ki67-positive cells). Survivin was found in all examined lymphomas belonging to 6 different morphological subtypes with nuclear immunoreactivity. In most of lymphomas (18/25) survivin expression ranged 10%-25% of positive cells. Only single cases had lower (0-10% positive cells, 1/25) or higher (25-50% and >50% positive cells, 5/25 and 1/25, respectively) index of survivin. Neither mitotic index nor proliferative index correlated with survivin expression when the values quantified randomly in whole specimens were compared. However, when survivin expression were quantified in selected tumor areas of low and high proliferation activity the high correlations between survivin expression and proliferation index were found. The results indicated that survivin is commonly expressed in canine lymphomas. Nuclear labelling together with the relation of its expression and proliferative activity in highly proliferative areas of neoplastic tissue suggest a potential role of survivin in cell cycle activation in canine lymphoma cells. However, further studies of the relation between expression of survivin and other proteins involved in cell cycle regulation are needed. Moreover, the results suggest that survivin may pose the therapeutic target in canine lymphomas.

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“…Schematic illustration of survivin expression patterns in skin structures during the various stages of the canine hair follicle cycle. The interfollicular epidermal and sebaceous gland data for Ki67 and survivin are derived from Bongiovanni et al3,11 Immunofluorescence in normal hair follicles. First column shows blue nuclei with 4′,6-diamidino-2-phenylindole counterstain.…”

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confidence: 99%

On the role of survivin as a stem cell biomarker of canine hair follicle and related tumours

Bongiovanni

Diodoro

Salda

et al. 2014

Veterinary Dermatology

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Nuclear survivin expression as a potentially useful tool for the diagnosis of canine cutaneous sebaceous lesions

Abstract: The level of survivin expression increased with increasing malignancy, designating survivin as a new diagnostic marker in the assessment of malignancy of sebaceous tumours.

Cited by 12 publications

References 30 publications

The Process of Apoptosis in a Holocrine Gland as Shown by the Avian Uropygial Gland

The Process of Apoptosis in a Holocrine Gland as Shown by the Avian Uropygial Gland

Survivin expression in canine lymphomas in relation with proliferative markers

On the role of survivin as a stem cell biomarker of canine hair follicle and related tumours

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