Nuclear sperm quality in total polymorphic teratozoospermia and its impact on intracytoplasmic sperm injection outcome

Braham, Asma; Ghédir, Houda; Zidi, Ines; Sallem, Amira; Hajlaoui, Amani; Ajina, Mounir; Saâd, Ali; Ibala-Romdhane, Samira

doi:10.1111/and.13252

Cited by 10 publications

(5 citation statements)

References 45 publications

(64 reference statements)

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“…This outcome is in accordance with a study by French and colleagues who have reported a pregnancy rate of 60% in absolute teratozoospermia patients compared to 57% in males with normal semen [27]. In contrast, a study by Braham and colleagues found that the clinical pregnancy rate for absolute teratozoospermia is only 10% [17]. Since the paternal genome switches on at the early embryonic stage, researchers have hypothesized that SDF, which tends to be higher in teratozoospermia patients, would lead to low pregnancy rates and high miscarriage rates; they have also expressed concern that selecting spermatozoa with abnormal morphology for ICSI would lead to the formation of chromosomally abnormal embryos [25].…”

Section: Discussionsupporting

confidence: 91%

“…By definition, donor oocytes are from women in their 20s or early 30s are likely capable of more extensive DNA repair. In contrast, the study by Braham and colleagues reported a 10% pregnancy rate after ICSI for couples with absolute teratozoospermia and live birth was not documented [17]. However, a study from a different perspective by Kovac demonstrated that 29.2% of men with 100% abnormal sperm structure conceived without assisted reproduction over a period of 2.5 years [18].…”

Section: Introductionmentioning

confidence: 94%

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DNA Integrity in Absolute Teratozoospermia Patients and its Impact on Assisted Reproductive Technology Outcome

Faris

2023

J Gynecol Clin Obstet Reprod Med.

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Numerous studies showed that Sperm DNA Fragmentation (SDF) is inversely correlated to semen parameters and that high SDF is a major contributor to failure of conception. In the present study, the severity of Sperm DNA Fragmentation in absolute teratoozoospermia patients and its impact on ICSI outcome compared to males with normal semen was examined. A prospective study was performed including 36 absolute teratozoospermia and 30 controls. The mean of DNA Fragmentation Index (DFI) using Sperm Chromatin Dispersion (SCD) test in the absolute teratozoospermia was 42.9 ± 13.2, compared to 24.59 ± 6.9 for the controls. The percentage of patients with high DFI (>30) was higher in the absolute teratozoospermia group 78% than in the control group 30%. Moderate DFI (15-30%) was more common in controls 60% than in absolute teratozoospermia 22%. Normal DFI (≤ 15%) was completely absent in absolute teratozoospermia. The pregnancy rate following Intracytoplasmic Sperm Injection (ICSI) in the control group was 66.6% and 50% in the absolute teratozoospermia. The miscarriage rate was 26% in the control group compared to 53% in the absolute teratozoospermia. Live birth was significantly lower in absolute teratozoospermia compared to fertile men; 19% and 52% respectively. High levels of SDF are predominant in absolute teratozoospermia patients consequently affecting the outcome of ICSI.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 94%

DNA Integrity in Absolute Teratozoospermia Patients and its Impact on Assisted Reproductive Technology Outcome

Faris

2023

J Gynecol Clin Obstet Reprod Med.

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“…Sperm DNA integrity of men with teratozoospermia has recently been the subject of intense research studies which reported that these subjects show high levels of sperm nuclear defects 3,4,8,36 . However, the origins of such defects are still a matter of debate.…”

Section: Discussionmentioning

confidence: 99%

Teratozoospermia: Its association with sperm DNA defects, apoptotic alterations, and oxidative stress

2020

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This study was carried out to evaluate the level of nuclear sperm DNA damage in men with isolated polymorphic teratozoospermia and examining its relationship with apoptosis and oxidative stress. A total of 89 subjects were divided into two groups: men with isolated teratozoospermia (n = 69) and men with normal semen parameters (n = 20) as controls. Sperm DNA breaks were determined by using acridine orange staining. The proportion of viable spermatozoa with mitochondrial transmembrane depolarization was detected by fluorescence microscopy through the use of MitoPTJC‐1 staining method. Bivariate Annexin V/6‐CFDA analysis was then set out in order to measure the percentage of both viable and dead spermatozoa with phosphatidylserine (PS) externalization. Seminal antioxidant profile (reduced glutathione (GSHr); oxidized glutathione (GSSG); glutathione S‐transferase (GST)) and total protein sulfhydryl (P‐SH) concentrations were measured spectrophotometrically. Men with isolated teratozoospermia, when compared to controls, showed significantly increased level of single sperm DNA breaks and higher proportions of spermatozoa with phosphatidylserine externalization and mitochondrial depolarization. Among the differently studied oxidative stress seminal parameters, the rates of seminal GSHr, GST, and P‐SH were significantly decreased in the teratozoospermic group. However, the seminal rates of GSSG and GST have decreased, but only GST did not show a significant difference. Interestingly, significant correlations were found between the studied apoptotic markers and the rate of atypical sperm forms with the incidences of head abnormalities. Furthermore, positive inter‐correlations were found between sperm DNA defects, impaired seminal antioxidant status, and the apoptotic sperm markers. Such data provide clear evidence that the apoptotic alterations are closely correlated to abnormal sperm morphology and DNA damage. Moreover, decreased seminal antioxidant profile may be a crucial factor involved in the mechanism of sperm cell death‐mediated DNA breaks in teratozoospermic semen.

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“…It is known that the quality of seminal samples is strictly related to the predisposition to chromosomal errors and to the incidence of abnormal sperm ( Bernardini et al ., 2000 ; Gianaroli et al , 2005 ). Moreover, infertile men have a higher frequency of gametes with altered DNA than fertile men and the frequency of anomalies increases proportionally to the severity of the male factor ( Bernardini et al ., 2000 ; 2004 ; 2005 ; Braham et al , 2019 ; Calogero et al ., 2003 ; Esquerré-Lamare et al ., 2018 ; Gianaroli et al ., 2005 ; Kodama et al ., 1997 ; Sakkas et al , 2003 ; Simon et al , 2017 ; Zini et al, 2001 ). In addition, even infertile men with normal seminal parameters may also show high DNA fragmentation ( Kodama et al ., 1997 ; Zini et al ., 2001 ).…”

Section: Introductionmentioning

confidence: 99%

Sperm selection by birefringence: a promising non-invasive tool to improve ICSI outcomes

Ribeiro¹,

Broi²,

Rose³

et al. 2022

JBRA

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Objective: Despite higher sperm DNA fragmentation may affect intracytoplasmic sperm injection (ICSI) outcomes, sperm selection protocols do not evaluate this parameter. Therefore, sperm's head birefringence has been suggested as an adjuvant of seminal processing to select viable sperm for couples with severe male factor. Considering men with normal seminal parameters may also curse with DNA fragmentation, the aim of this study was to evaluate the impact of sperm selection by birefringence on ICSI outcomes in couples with different infertility factors compared to those submitted to conventional sperm selection.Methods: In this case-control study, medical records from 181 couples who underwent ICSI from January 2018 to August 2020 (107 from the Conventional and 74 from the Birefringence group) were included in the study. Clinical characteristics and ICSI outcomes were compared between the groups using Student's t test or Chi-square test (p<0.05) and a multivariate logistic regression model was applied regarding clinical pregnancy.Results: Despite the Birefringence group showed higher female age (p=0.01), lower seminal sperm concentration (p<0.01) and higher sperm DNA fragmentation (p<0.01), those patients cursed with both higher cleavage rate (p=0.04), clinical pregnancy rate per transfer (p=0.03) and clinical pregnancy rate per initiated cycle (p=0.02). The logistic regression showed a positive group effect on clinical pregnancy. Conclusions:The findings suggest a positive clinical impact of this cheap and easily reproducible adjuvant technique on ICSI outcomes in couples with different infertility factors. If confirmed by further methodologically appropriate studies, the sperm's head birefringence could be considered to improve the reproductive chances of those patients.

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Nuclear sperm quality in total polymorphic teratozoospermia and its impact on intracytoplasmic sperm injection outcome

Cited by 10 publications

References 45 publications

DNA Integrity in Absolute Teratozoospermia Patients and its Impact on Assisted Reproductive Technology Outcome

DNA Integrity in Absolute Teratozoospermia Patients and its Impact on Assisted Reproductive Technology Outcome

Teratozoospermia: Its association with sperm DNA defects, apoptotic alterations, and oxidative stress

Sperm selection by birefringence: a promising non-invasive tool to improve ICSI outcomes

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