Nuclear Signaling of Plant MAPKs

Bigeard, Jean; Hirt, Heribert

doi:10.3389/fpls.2018.00469

Cited by 158 publications

(118 citation statements)

References 174 publications

(221 reference statements)

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“…Therefore, the stressinduced nuclear translocation of RGLG2 contributes to the degradation of a positive factor for stress tolerance, possibly as a desensitization mechanism. Different enzymes and signaling proteins shuttle to the nucleus in response to environmental cues or stress, which enables interactions with the target proteins (Lee et al, 2015;Bigeard and Hirt, 2018). Both RGLG1 and RGLG2 are plasma membrane-associated proteins with a predicted N-terminal myristoylation site (Yin et al, 2007;Figure S2).…”

Section: Introductionmentioning

confidence: 99%

ABA inhibits myristoylation and induces shuttling of the RGLG1 E3 ligase to promote nuclear degradation of PP2CA

et al. 2019

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Summary Hormone‐ and stress‐induced shuttling of signaling or regulatory proteins is an important cellular mechanism to modulate hormone signaling and cope with abiotic stress. Hormone‐induced ubiquitination plays a crucial role to determine the half‐life of key negative regulators of hormone signaling. For ABA signaling, the degradation of clade‐A PP2Cs, such as PP2CA or ABI1, is a complementary mechanism to PYR/PYL/RCAR‐mediated inhibition of PP2C activity. ABA promotes the degradation of PP2CA through the RGLG1 E3 ligase, although it is not known how ABA enhances the interaction of RGLG1 with PP2CA given that they are predominantly found in the plasma membrane and the nucleus, respectively. We demonstrate that ABA modifies the subcellular localization of RGLG1 and promotes nuclear interaction with PP2CA. We found RGLG1 is myristoylated in vivo, which facilitates its attachment to the plasma membrane. ABA inhibits the myristoylation of RGLG1 through the downregulation of N‐myristoyltransferase 1 (NMT1) and promotes nuclear translocation of RGLG1 in a cycloheximide‐insensitive manner. Enhanced nuclear recruitment of the E3 ligase was also promoted by increasing PP2CA protein levels and the formation of RGLG1–receptor–phosphatase complexes. We show that RGLG1Gly2Ala mutated at the N‐terminal myristoylation site shows constitutive nuclear localization and causes an enhanced response to ABA and salt or osmotic stress. RGLG1/5 can interact with certain monomeric ABA receptors, which facilitates the formation of nuclear complexes such as RGLG1–PP2CA–PYL8. In summary, we provide evidence that an E3 ligase can dynamically relocalize in response to both ABA and increased levels of its target, which reveals a mechanism to explain how ABA enhances RGLG1–PP2CA interaction and hence PP2CA degradation.

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Section: Introductionmentioning

confidence: 99%

ABA inhibits myristoylation and induces shuttling of the RGLG1 E3 ligase to promote nuclear degradation of PP2CA

et al. 2019

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“…To effectively combat attacks by these invaders, plants have evolved a diverse array of defense mechanisms, which include preexisting physical barriers and inducible defense responses that are activated upon attack 1 . The induced defense mechanisms are usually associated with reactive oxygen species (ROS) 2 , mitogenactivated protein kinase (MAPK) cascades 3 and signaling modulated by phytohormones, such as jasmonic acid (JA), salicylic acid (SA), abscisic acid (ABA), and ethylene (ET).…”

Section: Introductionmentioning

confidence: 99%

(E)-Nerolidol is a volatile signal that induces defenses against insects and pathogens in tea plants

et al. 2020

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Plants release large amounts of volatile organic compounds (VOCs) in response to attackers. Several VOCs can serve as volatile signals to elicit defense responses in undamaged tissues and neighboring plants, but many questions about the ecological functions of VOCs remain unanswered. Tea plants are impacted by two harmful invaders, the piercing herbivore Empoasca (Matsumurasca) onukii Matsuda and the pathogen Colletotrichum fructicola. To determine the VOC signals in tea, we confirmed CsOPR3 as a marker gene and set up a rapid screening method based on a 1.51 kb CsOPR3 promoter fused with a β-glucuronidase (GUS) reporter construct (OPR3p::GUS) in Arabidopsis. Using this screening system, a terpenoid volatile (E)-nerolidol was identified as a potent signal that elicits plant defenses. The early responses triggered by (E)-nerolidol included the activation of a mitogen-activated protein kinase and WRKY, an H 2 O 2 burst, and the induction of jasmonic acid and abscisic acid signaling. The induced plants accumulated high levels of defenserelated chemicals, which possessed broad-spectrum anti-herbivore or anti-pathogen properties, and ultimately triggered resistance against Empoasca onukii and Colletotrichum fructicola in tea. We propose that these findings can supply an environmentally friendly management strategy for controlling an insect pest and a disease of tea plants.

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“…MPK3 and MPK6 are very well-known stress-responsive MAPKs. Many of their substrates have been identified in the context of Microbe-Associated Molecular Pattern (MAMP) signaling (Bigeard et al, 2015; Rayapuram et al, 2018; Bigeard and Hirt, 2018). However, very little is known about their functions in response to other stresses and notably in response to wounding.…”

Section: Discussionmentioning

confidence: 99%

Wounding and insect feeding trigger two independent MAPK pathways with distinct regulation and kinetics

Sözen

Schenk

Boudsocq

et al. 2019

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AbstractWounding is caused by abiotic and biotic factors and triggers complex short- and long-term responses at the local and systemic level. These responses are under the control of complex signaling pathways, which are still poorly understood. Here, we show that the rapid activation of MKK4/5-MPK3/6 by wounding is independent of jasmonic acid (JA) signaling and that, contrary to what happens in tobacco, this fast module does not control wound-triggered JA accumulation in Arabidopsis. We also demonstrate that a second MAPK module, constituted by MKK3 and the clade-C MAPKs MPK1/2/7, is activated by wounding in an independent manner. We provide evidence that the activation of this MKK3-MPK1/2/7 module occurs mainly through wound-induced JA production via the transcriptional regulation of upstream clade-III MAP3Ks and particularly MAP3K14. We show that mkk3 mutant plants are more susceptible to the larvae of the generalist lepidopteran herbivore Spodoptera littoralis, indicating that the MKK3-MPK1/2/7 module is involved in counteracting insect feeding.One sentence summaryWounding induces the parallel activation of a rapid signaling module (MKK4/5-MPK3/6) and a JA-dependent slow one (MAP3K14-MKK3-MPK1/2/7/14) to restrict insect feeding.

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Nuclear Signaling of Plant MAPKs

Cited by 158 publications

References 174 publications

ABA inhibits myristoylation and induces shuttling of the RGLG1 E3 ligase to promote nuclear degradation of PP2CA

ABA inhibits myristoylation and induces shuttling of the RGLG1 E3 ligase to promote nuclear degradation of PP2CA

(E)-Nerolidol is a volatile signal that induces defenses against insects and pathogens in tea plants

Wounding and insect feeding trigger two independent MAPK pathways with distinct regulation and kinetics

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