Nuclear magnetic resonance studies on poly(β-hydroxybutyrate) and a copolyester of β-hydroxybutyrate and β-hydroxyvalerate isolated from Alcaligenes eutrophus H16

Doi, Yoshiharu; Kunioka, Masao; Nakamura, Yoshiyuki; Soga, Kazuo

doi:10.1021/ma00165a033

Cited by 231 publications

(116 citation statements)

References 2 publications

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“…The main bands and peaks of the FTIR and 1 H NMR spectra correspond to PHB, according to those found in the literature [42]. Furthermore, the chemical signals obtained in the present work agree with those obtained by [43] for a PHB produced by fermentation. These results together with the FT-IR analysis showed that strain SA8 was able to accumulate PHB using glycerol as a substrate.…”

Section: Discussionsupporting

confidence: 90%

Bioconversion of Commercial and Waste Glycerol into Value-Added Polyhydroxyalkanoates by Bacterial Strains

Castro¹

2014

J Microb Biochem Technol

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The industrial conversion of renewable resources into bio-based materials has been increasingly receiving attention from an economic and, in particular, an environmental point of view. Among these bioresources, glycerol represents one of the most important base materials for the manufacturing of bulk and higher-valued products. This is mainly based on the increasing amounts of waste glycerol due to the rising production of biodiesel and other oleochemicals, as well as on the applicability of glycerol as a water-soluble carbon source for a wide range of microbial productions. Polyhydroxyalkanoates (PHAs) are biodegradable and environmentally friendly biopolymers, and the major contributor to PHA production is the substrate cost, therefore it is desirable to produce PHA from waste residues like biodiesel byproducts. Since soil ecosystems have a rich, but still insufficiently studied microbial flora, the ability of bacteria isolated from Finnish soils and sediments were investigated for the production of valueadded materials such as Polyhydroxyalkanoates based on pure and waste glycerol as carbon source. 1 out of 40 isolated strains was selected for further studies based on ability to produce PHA on mineral medium and identified as Halomonas sp SA8 with up to 56% of Polyhydroxyalkanoate accumulation. The produced biopolymer was recovered and identified as PHB homopolymer.

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Section: Discussionsupporting

confidence: 90%

Bioconversion of Commercial and Waste Glycerol into Value-Added Polyhydroxyalkanoates by Bacterial Strains

Castro¹

2014

J Microb Biochem Technol

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“…The 13 C NMR analysis supported the results from the proton NMR analysis. The resonance of the monomers at 19.87 (9), 40.80 (7), 67.64 (8), and 169.26 (1) ppm were assigned by data comparison to 3HB according to the previous report (Doi et al, 1986), whereas the resonances at 22.10 (4), 38.65 (2) and 71.91 ppm were referred to 3HV. In contrast, 1 H NMR spectral analyses of the purified polymer from the recombinant yeast strain INVSc1/ PHA1 revealed the presence of 3, 2 and 1 protons at chemical shifts 1.2, 2.4-2.6 and 5.3, respectively (Fig.…”

Section: Pha Analysismentioning

confidence: 99%

Biosynthesis of biodegradable polyhydroxyalkanotes biopolymers in genetically modified yeasts

Abd‐El‐Haleem

Amara

Zaki

et al. 2007

Int. J. Environ. Sci. Technol.

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ABSTRACT:In the recent decade, biosynthesis of the degradable biopolymers polyhydroxyalkanotes in transgenic yeasts became an important research task. Most research strategies depend on either metabolic engineering or molecular approaches. In the present work, research compared PHA biosynthesis in two types of yeasts; Saccharomyces cerevisiae and a non-convenient Kloeckera spp. Yeast strains were equipped in their cytoplasm with the phaABC Re operon containing genes phbA, phbB and phbC of the PHA biosynthetic pathway of Ralstonia eutropha, which encode â-ketothiolase, NADPH-linked acetoacetyl-CoA reductase and PHA synthase, respectively. The transgenic strains Saccharomyces cerevisiae and Kloeckera sp. were able to produce PHA. The maximum content of the polymer detected in the recombinant strain INVSc1/PHA1 was 2.68 % and only poly-3-hydroxybutyrate (PHB) accumulated. However, the non-conventional transgenic strain KY1/PHA was able to accumulate as maximum of 7.06 % of the copolymer poly-(3-hydroxybutyrateco-poly-3-hydroxyvalerate) (PHV). Western blot analysis confirmed expression of the phaABC Re operon in the transgenic yeast strains. The nature of the PHA thus produced by all tested strains was analyzed by 1 H and 13 C nuclear magnetic resonance (NMR) spectroscopy.

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“…Analytical methods. PHB was extracted from cells by the method of Doi et al (11) and converted to crotonic acid as described by Law and Slepecky (24). Crotonic acid was quantified by HPLC on a Polypore H column (4.6 mm by 250 mm; Brownlee, Kontron, Eching, Germany) in accordance with the procedure of Karr et al (20).…”

Section: Microscopy Of Cell Suspensions and Stained Cellsmentioning

confidence: 99%

Isolation and Characterization of a New Gram-Negative, Acetone-Degrading, Nitrate-Reducing Bacterium from Soil, Paracoccus solventivorans sp. nov.

Siller

Rainey²,

Stackebrandt³

et al. 1996

International Journal of Systematic Bacteriology

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An acetone-degrading, nitrate-reducing, coccoid to rod-shaped bacterium, strain L1, was isolated from soil on the site of a natural gas company. Cells of the logarithmic growth phase reacted gram positive, while those of the stationary growth phase were gram negative. Single organisms were 0.4 to 0.5 by 0.9 to 1.5 pm in size, nonmotile, and non-spore forming and had poly-P-hydroxybutyrate inclusions. The doubling time of strain L1 on acetone-C0,-nitrate at the optimal pH of 7 to 8 and the optimal temperature of 30 to 37°C was 12 h. More than 0.2% NaCl or 10 mM thiosulfate inhibited growth. For oxygen or nitrate respiration, acetone and a few organic chemicals were utilized as carbon sources whereas many others could not be used (for details, see Results). Bicarbonate (or CO,) was essential for growth on acetone but not for growth on acetoacetate. The growth yields for acetone-CO, and acetoacetate were 28.3 and 27.3 g/mol, respectively. With acetone as the carbon source, poly-P-hydroxybutyrate accounted for up to 40% of the cellular dry weight. The DNA of strain L1 had a G+C content of 68.5 mol% (as determined by high-performance liquid chromatography of nucleotides) or 70 mol% (as determined by the T, method). The sequence of the gene coding for the 16s rRNA led to the classification of strain L1 in the paracoccus group of the alpha subclass of the Proteobucteriu. The new isolate is named Paracoccus solventivuruns sp. nov. DSM 6637.Acetone is a fermentation product of fungi (15), clostridia (see, for example, reference 43), and several other bacteria (6, 35,36,51,57,58). It is also excreted into the environment in ripening apples as a bioconversion product derived from p pvate. Commercially, acetone is produced from isopropanol, cumene, or propane to serve as a solvent for resins, fats, oil, plastics, waxes, etc. (30). Acetone may therefore occur in many ecosystems as a naturally produced substance or as an industrial pollutant. It is, however, not accumulating because of its degradability by aerobic (see, e.g., references 25 and 27) and anaerobic (see, e.g., references 28, 36, and 38) bacteria. Because of its biological degradability in the presence or absence of oxygen, it is an especially suitable, nontoxic elutant for extraction of polycyclic aromatic hydrocarbons (PAHs) from contaminated soil for bioremediation. After extraction, the acetone-PAH solution can be easily separated by evaporation or distillation of the solvent. The acetone residue in the soil is harmless because of its rapid biological degradation. In an attempt to study PAH degradation in contaminated soil Samples, PAHs were extracted with acetone. In the first enrichments, nitrate-reducing, acetone-degrading bacteria were obtained. From these enrichments, a new acetone-degrading organism, strain L1, was isolated and subsequently characterized in detail.MATERIALS AND METHODS Sources of organisms. Strain L1 was isolated from a soil sample taken at a depth of 1 m at the site of a defunct natural gas company. (2) and dispensed in an anaerobic c...

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Nuclear magnetic resonance studies on poly(β-hydroxybutyrate) and a copolyester of β-hydroxybutyrate and β-hydroxyvalerate isolated from Alcaligenes eutrophus H16

Cited by 231 publications

References 2 publications

Bioconversion of Commercial and Waste Glycerol into Value-Added Polyhydroxyalkanoates by Bacterial Strains

Bioconversion of Commercial and Waste Glycerol into Value-Added Polyhydroxyalkanoates by Bacterial Strains

Biosynthesis of biodegradable polyhydroxyalkanotes biopolymers in genetically modified yeasts

Isolation and Characterization of a New Gram-Negative, Acetone-Degrading, Nitrate-Reducing Bacterium from Soil, Paracoccus solventivorans sp. nov.

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