Biosynthesis of biodegradable polyhydroxyalkanotes biopolymers in genetically modified yeasts

Abd‐El‐Haleem, Desouky; Amara, Amro A.; Zaki, Sahar; Abulhamd, Ashraf; Abulreesh, G.

doi:10.1007/bf03325988

Cited by 19 publications

(8 citation statements)

References 26 publications

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“…These spectra show PHB polymer characteristic signs and other signs that show sample possible contamination. The results agreed with those reported by Doi et al (1986) and Abd-El-haleem et al (2007). Terminal unit signals (C 1, 3,4,5, 23, 26) were not observed, possibly due to their low molar ratio in the copolymer.…”

Section: Nuclear Magnetic Resonance (Nmr)supporting

confidence: 94%

Characterization of a polyhydroxyalkanoate obtained from pineapple peel waste using Ralsthonia eutropha

Vega‐Castro

Contreras‐Calderón

León

et al. 2016

Journal of Biotechnology

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Section: Nuclear Magnetic Resonance (Nmr)supporting

confidence: 94%

Characterization of a polyhydroxyalkanoate obtained from pineapple peel waste using Ralsthonia eutropha

Vega‐Castro

Contreras‐Calderón

León

et al. 2016

Journal of Biotechnology

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“…Different agricultural wastes can be used for this purpose through chemical or enzymatic hydrolysis (Du et al 2012;Sandhya et al 2013). Mono-and disaccharides can be used for the production of PHAs mainly in aseptic cultivation because it is possible to select strains of bacteria (Du et al 2012;Vishnuvardhan and Thirumala 2012;Yee et al 2012) or yeasts (Abd-El-Haleem et al 2007) with low production of storage carbohydrates under excess of carbon and energy source. Meanwhile, the wild strains of microorganisms in non-aseptic mixed culture will transform excess of external sugars to the glycogen, exopolysaccharides, or lipids (Ivanov 2010).…”

Section: Problems Of Pha Productionmentioning

confidence: 99%

Production and applications of crude polyhydroxyalkanoate-containing bioplastic from the organic fraction of municipal solid waste

Ivanov

Stabnikov

Ahmed

et al. 2014

Int. J. Environ. Sci. Technol.

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A considerable economic and environmental need exists for the further development of degradable plastic polyhydroxyalkanoates (PHAs), which are produced by bacteria. However, the production cost of this bioplastic, manufactured using conventional technologies, is several times higher than that of petrochemical-based plastics. This is a major obstacle for the industrial production of PHA bioplastic for non-medical use. The aim of this review is to evaluate suitable methods for the significant reduction in bioplastic production costs. The study findings are as follows: (1) The organic fraction of municipal solid waste can be used as a raw material through acidogenic fermentation; (2) non-aseptic cultivation using mixed bacterial culture can significantly reduce the production cost; (3) biotechnology of bacterial cultivation should ensure selection of PHA-accumulating strains; (4) applications of PHAcontaining material in both construction industry and agriculture do not require expensive extraction of PHAs from bacterial biomass. The implementation of the above findings in the current manufacturing process of PHAcontaining bioplastic would significantly reduce production costs, thereby rendering PHA-containing bioplastic an economically viable and environmentally friendly alternative to petrochemical-based plastics.Keywords Bioplastic Á Polyhydroxyalkanoates Á Municipal solid waste Á Construction material Biodegradable plastics Biodegradable plasticQuantity of plastics in municipal solid waste (MSW) in USA is 27 million tons (US EPA 2011). The major portion of these materials is incinerated or landfilled, which both are not-sustainable and environmentally not-friendly solutions. A considerable economic and environmental need exists for the further development of biodegradable plastics that will be transformed to carbon dioxide and water being landfilled or disposed in the environment. Additional advantage of biodegradable plastics is that many of them are produced biologically from renewable sources so the production of these bioplastics will increase environmental and economic sustainability. However, the production cost of the bioplastics, manufactured using conventional technologies, is several times higher than that of petrochemical-based plastics. Therefore, reduction in the bioplastic production cost due to the usage of cheap raw materials and technological innovations is essential for the bioplastic production and applications. Content of accumulated PHAs can be up to 80 % of dry biomass. These substances can be extracted from bacterial biomass and used in practice as a plastic with the melting temperature 160-180°C, tensile strength 24-40 MPa, and elongation at break 3-142 %. Lastly, elasticity of the PHA plastic depends on the content of PHV in PHAs. These properties are comparable with the properties of petroleum-based thermoplastics. The most common commercial PHAs consist of a copolymer PHB and PHV together with a plasticizer/softener and inorganic additives such as titanium dioxide and calciu...

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“…Another important example is the genes encoding the polyhydroxybutyrate (PHB) biosynthetic pathway of Ralstonia eutropha is transferred into industrially convenient organism Saccharomyces cerevisiae [15].…”

Section: Heterologous Expression Of Entire Gene Clustermentioning

confidence: 99%

Metabolic Engineering of Bacteria

Kumar¹,

Prasad²

2011

Indian J Microbiol

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Yield and productivity are critical for the economics and viability of a bioprocess. In metabolic engineering the main objective is the increase of a target metabolite production through genetic engineering. Metabolic engineering is the practice of optimizing genetic and regulatory processes within cells to increase the production of a certain substance. In the last years, the development of recombinant DNA technology and other related technologies has provided new tools for approaching yields improvement by means of genetic manipulation of biosynthetic pathway. Industrial microorganisms like Escherichia coli, Actinomycetes, etc. have been developed as biocatalysts to provide new or to optimize existing processes for the biotechnological production of chemicals from renewable plant biomass. The factors like oxygenation, temperature and pH have been traditionally controlled and optimized in industrial fermentation in order to enhance metabolite production. Metabolic engineering of bacteria shows a great scope in industrial application as well as such technique may also have good potential to solve certain metabolic disease and environmental problems in near future.

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Biosynthesis of biodegradable polyhydroxyalkanotes biopolymers in genetically modified yeasts

Cited by 19 publications

References 26 publications

Characterization of a polyhydroxyalkanoate obtained from pineapple peel waste using Ralsthonia eutropha

Characterization of a polyhydroxyalkanoate obtained from pineapple peel waste using Ralsthonia eutropha

Production and applications of crude polyhydroxyalkanoate-containing bioplastic from the organic fraction of municipal solid waste

Metabolic Engineering of Bacteria

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