Nuclear magnetic resonance spectroscopy

Abstract

The range of problems in clinical chemistry that can be addressed by MRS is wide. The number of applications reported in the literature is growing steadily, particularly since the study of the composition of physiological fluids and tissues, and the changes thereof in disease, are well suited to study by MRS. Moreover, the major technical limitations that have impeded its progress into the clinical laboratory in the past have been addressed. Recent hardware and software developments have further improved and simplified MRS analysis. Thus, it would be surprising if MRS of physiological fluids and tissues does not become an essential technique for clinical chemists and pathologists. In practice, three main obstacles remain to be overcome: a greater availability of instruments, a larger data base of spectral changes correlated with pathological conditions, and an enhanced supply of MR-trained individuals in the clinical environment.

Asthma Metabolomics and the Potential for Integrative Omics in Research and the Clinic

“…NMR uses the magnetic properties of atomic nuclei to generate information on structure and thereby identify metabolites in the biofluid under investigation by their unique pattern of chemical shifts and peak intensities. 28 Liquid or gas chromatography tandem mass spectrometry combines chromatography, a technique that separates metabolites, with MS, which measures their abundance. The complement of metabolites measured by NMR and MS may not always be comparable.…”

1H NMR Analysis for Metabolites in Serum and Urine from Rats Administrated Chronically with La(NO3)3

“…The glucose content is lowered when the dose is higher (10, 20 mg La(NO 3 ) 3 /kg body wt). TMAO alone may not be a reliable marker of renal dysfunction, but a high excretion of TMAO appears to be associated with renal failure (30). Figure 2b shows the 1 H NMR spectrum of urine in rats receiving 10 mg/kg body wt of La(NO 3 ) 3 after 3 months.…”

Directly Coupled High-Performance Liquid Chromatography and Nuclear Magnetic Resonance Spectroscopic with Chemometric Studies on Metabolic Variation in Sprague–Dawley Rats

“…Complex metabolic profiles, which relate to the physiological or pathological status of an organism, can be generated via high-resolution 1 H NMR spectroscopic analysis of biofluids (1,2). This technique, which has been applied to the elucidation of both endogenous and drug-related metabolites, has proved an effective method for monitoring disease states and has been used to study the metabolism and toxicity of pharmacologically active agents (1)(2)(3)(4)(5)(6).…”

“…This technique, which has been applied to the elucidation of both endogenous and drug-related metabolites, has proved an effective method for monitoring disease states and has been used to study the metabolism and toxicity of pharmacologically active agents (1)(2)(3)(4)(5)(6).…”

“…Assignment of biofluid composition can be aided by the combination of chromatographic techniques such as solid-phase extraction chromatography (SPEC) 2 and directly coupled HPLC-NMR spectroscopy (8 -10), or by the application of advanced multivariate statistical analysis (11)(12)(13). Directly cou-pled HPLC-NMR is now a well-established technique that has been applied to the separation and structural elucidation of unknown compounds of both endogenous and xenobiotic origin (8 -10).…”