Nuclear inclusions observed by electron microscope in cynomolgus monkey kidney cells infected with influenza virus

Saito, Y; Yoshioka, Isao; Igarashi, Y; Nakagawa, Susumu

doi:10.1016/0042-6822(70)90420-4

Cited by 10 publications

(7 citation statements)

References 5 publications

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“…Furthermore, several authors observed in the nucleus additional elongated tubular structures of 40-50 m diameters, of various lengths (up to 1 mm max length) with periodic internal dense striation 14,17,18 (Fig. 3D-F).…”

Section: Influenza a Viruses Induce Strong Remodeling Of The Host Nucmentioning

confidence: 92%

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Influenza A replication and host nuclear compartments: Many changes and many questions

Josset

Frobert

Rosa‐Calatrava

2008

Journal of Clinical Virology

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Section: Influenza a Viruses Induce Strong Remodeling Of The Host Nucmentioning

confidence: 92%

“…[12][13][14][15] During infection, the architecture of the nucleolus is deeply disrupted, as observed in Fig. 3 in comparison with mock-infected cell ( Fig.…”

Section: Influenza a Viruses Induce Strong Remodeling Of The Host Nucmentioning

confidence: 97%

Influenza A replication and host nuclear compartments: Many changes and many questions

Josset

Frobert

Rosa‐Calatrava

2008

Journal of Clinical Virology

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“…It appears that the small amounts of RNP were co-purified with the crystals because, like other crystalline materials, they remained in the aqueous phase during extraction with fluorocarbon [12], Although no evidence for the presence of virus RNA was obtained by isotopic labeling, it is possible that any RNA that might have been present in the helices was degraded or that RNA was present in amounts below the level of detection by the analytical procedures employed. Structures with the appearance of loosely coiled helices were previously ob served in whole-mount preparations of detergent-treated purified virus [16], while the cylindrical particles enclosing a helix have been observed in both the nucleus and cytoplasm of intact, infected cells [1,2,6,7,17]. Judging by the results from both electron microscopy and gel analysis, there was a cor relation between the ratios of crystalline to helical material and NS to NP pro tein.although the proportion of the minor NP protein fraction was somewhat variable from one preparation to another.…”

Section: Discussionmentioning

confidence: 99%

“…The formation of such inclusions in a variety of cell lines in vitro and in mouse brain during infection by several strains of influenza virus has been described by others [1][2][3][4][5][6][7], Because there is both an abundance of virus ribonucleoprotein (RNP) in the infected cell and a prevalence of material giving positive immunofluorescence with antisera to RNP, others have speculated that the cytoplasmic crystals might represent an…”

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confidence: 99%

Characterization of Cytoplasmic Inclusions Formed during Influenza/WSN Virus Infection of Chick Embryo Fibroblast Cells

Morrongiello¹,

Dales²

1977

Intervirology

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Cytoplasmic crystalline inclusions could be isolated and partially purified from infiuenza/WSN-infected chick fibroblasts by extraction of the cytoplasmic homogenate with fluorocarbon, followed by sedimentation to equilibrium in potassium tartrate gradients. Repeated analyses of the crystal-enriched fraction by PAGE showed it to be predominantly protein (mol. wt. 23,000 daltons) with a minor contamination of 2–10% by a protein of mol. wt. 60,000 daltons, which comigrated with the NP protein. The latter is believed to exist within characteristic cytoplasmic and nuclear helices, also described in this study. The molecular weight data suggest that the crystals are composed of the so-called nonstructural protein.

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“…CHEN and SHIKATA [9] 960 (940) 1,200-1,400 (1,800-2,100) ADOLPH and HASELKORN [1] 550 SAITO et al [47] 400-500 O'LoUGHLIN et al [36] 320-350 2,000-4,000 WOLANSKI and CHAMBERS [61] 730 1,320 LEE [303 800 1,700 (900) MACLEoD [32] 500 2,500 BAYER and ~MSEN [3] 236 -4-31 1,030 4-107 MURPHY et al [35] 680 2,260 BRADLEY [5] 500--600…”

Section: Authormentioning

confidence: 96%

Gas-bacuoles and other viruslike structures in blue-green algae

Fjerdingstad

1972

Schweiz. Z. Hydrologie

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The result of electron microscopic investigations of gas-vacuoles in a culture of the benthal alga Oscillatoria chalybea was compared with the extensive literature concerning gas-vacuole formation and virus infection in bacteria and animals.On the basis of 21 features indicating that gas-vacuoles are pathological inclusions it was concluded that they are viruslike particles.Viruslike structures have also been found in our material of Symploca muscorum, Plectonema boryanum, a Nostoc sp., and a new case of virus infection in the thermopldlic alga Mastigocladus laminosus has been described.In a previous paper the author [15] pointed out that light microscopic features such as formation of calyptra, constrictions at the cross walls, cyanophycin granulesoften arranged at the cross walls -, sheath formation, and the direction in whichliving trichomes turn, are features which are without taxonomic value. Further it says in the paper that another problem to be solved is the associate bacteria, fungi, and viruses and their influence on the habitual features of the blue-green algae. So far, we know very little about the morphological effect of such associates, but there are probably many morphological features which might be explained if we understood the influence of associated microorganisms, cp. also FOREST [18].During the last few years the author, therefore, has also used electron microscopes for his investigations of blue-green algae. These investigations have confirmed what alredy GEITLER [20] wrote in his manual of Cyanophyceae that vacuole formations in the blue-green algae amount to a pathological change, and it should be added that this change may be caused by bacteria, fungi or viruses.Quite a number of the blue-green algae shown in literature are clearly marked by the effect of this pathological influence. In the cases where these pathological features are not part of the diagnosis of the species, they are of no importance for the taxonomy, but it must be pointed out that the pathological changes are in many cases included in and form an essential part of the diagnosis of the species.It appears evident that a pathological change of features cannot ]ustify'a separate taxon.

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Nuclear inclusions observed by electron microscope in cynomolgus monkey kidney cells infected with influenza virus

Cited by 10 publications

References 5 publications

Influenza A replication and host nuclear compartments: Many changes and many questions

Influenza A replication and host nuclear compartments: Many changes and many questions

Characterization of Cytoplasmic Inclusions Formed during Influenza/WSN Virus Infection of Chick Embryo Fibroblast Cells

Gas-bacuoles and other viruslike structures in blue-green algae

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