Nuclear glycoproteins of hamster liver and kirkman-robbins hepatoma recognized by concanavalin A

Lipińska, Anna; Gaczyński, M.

doi:10.1016/0020-711x(92)90063-7

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…The oocyte nucleoplasm of wild specimens expressed sialilated glycans (MAL II and SNA reactivity) from perinucleolus stage to cortical alveoli stages (S3 and S4), mannosilated, N‐acetylglucosylated, and fucosylated oligosaccharides from perinucleolus stage to late vitellogenic stage (Con A, GSA II, UEA I, and LTA affinity) and α‐galactosylated glycans (GSA I‐B 4 binding) from perinucleolus stage to early maturation stage. The presence of glycoproteins has been reported in the nucleus of cultured cells (Codogno et al,1992; Ferraro et al,1991; Stein et al,1975), in the liver of different animals (Ferraro et al,1991; Krzeslak and Lipinska,2002; Lipinska and Gaczynski,1992), as well as in the granulosa cells of lizard ovarian follicles (Hubert et al,1985; Seve et al,1984). The functional role of nuclear glycoproteins is not well known.…”

Section: Discussionmentioning

confidence: 98%

Lectin‐binding pattern of the senegalese sole Solea senegalensis oogenesis

Accogli

Zizza

García-López

et al. 2012

Microscopy Res & Technique

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The glycoconjugate pattern of developing ovarian follicles in wild and cultured Senegalese sole Solea senegalensis was investigated by means of lectin histochemistry. Ovaries from cultured fish contained oocytes up to the late vitellogenic stage, whereas they reached the hydration stage in wild specimens. The follicular cells bound MAL II, SBA, HPA, DBA, Con A, KOH-sialidase (K-s)-WGA, GSA I-B(4) in the late vitellogenic stage, and in wild fish also SNA and K-s-PNA, whereas in the hydration stage SBA, HPA, DBA, and GSA I-B(4) only. The zona radiata reacted with SBA, HPA, DBA, Con A, and GSA I B(4) in the late vitellogenic stage and in cultured fish also with UEA I, whereas in the hydration stage it stained with SBA only. The cortical alveoli bound SBA, HPA, RCA(120) during the late vitellogenic stage, also SNA, PNA, K-s-PNA, GSA I-B(4) in cultured fish, DBA, and K-s-WGA in wild ones which stained with SBA, HPA, and GSA I-B(4) in the hydrated stage. The yolk reacted with Con A in the late vitellogenic oocytes, and also with MAL II, SNA, K-s-PNA, SBA, HPA, K-s-WGA, GSA I-B(4), UEA I in the hydrated ones. From perinucleolus to late vitellogenic stages, the oocyte nucleoplasm bound Con A, GSA I-B(4), GSA II, UEA I, and in wild fish also MAL II, SNA, LTA but only GSA I-B(4) reactivity in the early maturation stage. These findings demonstrate that the glycan pattern of fish ovarian follicles changes during the maturative stages and that it is affected by culture-rearing conditions.

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