Nuclear genome stability of Mammillaria san-angelensis (Cactaceae) regenerants induced by auxins in long-term in vitro culture

Palomino, Guadalupe; Doležel, Jaroslav; Cid, Rocío; Brunner, Ingrid; Méndez, Ignacio; Rubluo, A.

doi:10.1016/s0168-9452(98)00216-7

Cited by 59 publications

(56 citation statements)

References 39 publications

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“…Therefore, the nuclear DNA content of in vitro-propagated E. planum was maintained during long-term in vitro culture (12 subcultures). Similar genome size stability has been reported in other taxons propagated in vitro, including medical plants such as Oenothera paradoxa, Inula verbascifolia, Rubus chamaemorus, Solidago virgaurea and S. graminifolia (Winkelmann et al 1998;Palomino et al 1999;Sliwinska and Thiem 2007). In contrast, in vitro clones of Pueraria lobata have a lower nuclear DNA content than control seedlings (Sliwinska and Thiem 2007).…”

Section: Establishment Of Root Culturessupporting

confidence: 76%

Phenolic acid and DNA contents of micropropagated Eryngium planum L.

Thiem

Kikowska

Krawczyk³

et al. 2013

Plant Cell Tiss Organ Cult

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A protocol for in vitro production of genetically uniform populations of the medicinal plant Eryngium planum, rich in selected phenolic acids, has been established. Shoot-tips were collected from axenic seedlings and grown on a Murashige and Skoog basal medium supplemented with 6-Benzyladenine (BA) and Indole-3-acetic acid (IAA). The highest shoot proliferation efficiency (17 shoots per explant) was obtained when 1.0 mg L -1 BA and 0.1 mg L -1 were added. Proliferating shoots were rooted and transferred to soil (89 % frequency of survival). Flow cytometric analysis of intact (field-grown) and microrpropagated plants revealed that all plants were uniform in genome size and had similar DNA contents. Thin-layer chromatography (TLC) analysis indicated that multiple shoots and roots from in vitro-derived plants produced high amounts of phenolic acids, primarily of rosmarinic acid (RA). Levels of phenolic acids in in vitroderived plants were similar to those of intact plants. Furthermore, high-performance liquid chromatography revealed that root cultures in liquid medium accumulated substantial levels of RA. Thus, rapid establishment of in vitro-grown organ cultures of E. planum can also serve as reliable sources for bioactive compounds.

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Section: Establishment Of Root Culturessupporting

confidence: 76%

Phenolic acid and DNA contents of micropropagated Eryngium planum L.

Thiem

Kikowska

Krawczyk³

et al. 2013

Plant Cell Tiss Organ Cult

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“…One of the possible reasons for the differences between plant cells growing in vitro and in vivo is that in vitro cultivation may induce genetic diversification. Genetic instability, known as somaclonal variation, is especially pronounced if an undifferentiated callus phase is used [6]. Several factors have been found to influence somaclonal variation including type and concentration of applied plant growth regulators [7], degree of endopolyploidy of explants [8], duration of in vitro cultivation [9] and ploidy level, genotype and type of explant [10].…”

mentioning

confidence: 99%

In vitro regeneration ability of diploid and autotetraploid plants of Cichorium intybus L.

2014

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“…Micropropagation has been a tool to rescue endangered plants (Moura-Costa et al 1993, Palomino et al 1999). In C. ipecacuanha callus culture were performed to produce alkaloids (Teshima et al 1988), and studies in vitro to obtain the whole plants was successful (Jha andJha 1989, Ideda et al 1989).…”

Section: Discussionmentioning

confidence: 99%

Reproductive Studies in Ipecac (Cephaelis ipecacuanha (Brot.) A. Rich; Rubiaceae): Flower Bud and Anther Size Associated to Male Gamete Development Stages

et al. 2003

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Summary Reproductive studies were carried out on Brazilian accessions of Cephaelis ipecacuanha, which is considered a threatened species. Meiotic behavior was studied using squashing technique with 1% acetic carmine and floral buds and anther were measured for the following male gamete development stages: meiosis I, meiosis II, tetrads, microspores and pollen. Bud and anther length showed significant differences (PϽ0.01) among the stages of pollen development. There was high significant correlation between bud and anther length by Pearson correlation. Tukey test results indicated that each analyzed stage could be associated to bud length mean but it was not found the same association for anther length.

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Nuclear genome stability of Mammillaria san-angelensis (Cactaceae) regenerants induced by auxins in long-term in vitro culture

Cited by 59 publications

References 39 publications

Phenolic acid and DNA contents of micropropagated Eryngium planum L.

Phenolic acid and DNA contents of micropropagated Eryngium planum L.

In vitro regeneration ability of diploid and autotetraploid plants of Cichorium intybus L.

Reproductive Studies in Ipecac (Cephaelis ipecacuanha (Brot.) A. Rich; Rubiaceae): Flower Bud and Anther Size Associated to Male Gamete Development Stages

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