In vitro regeneration ability of diploid and autotetraploid plants of Cichorium intybus L.

Ravandi, E. Ghotbi; Rezanejad, Farkhondeh; Dehghan, Esmail

doi:10.3103/s0095452714030050

Cited by 9 publications

(6 citation statements)

References 29 publications

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“…With these advantages, mass phenotyping and characterizing of new plants are now a possible task. Our study is in agreement with that of the induced polyploid plants of Cichorium intybus ( Ravandi, Rezanejad & Dehghan, 2014 ) and Dendrobium officinale ( Pham et al, 2019 ). The explants of induced polyploid of D. officinal produced a higher protocorm induction rate but required a longer time for protocorm initiation.…”

Section: Discussionsupporting

confidence: 92%

“…The explants of induced polyploid of D. officinal produced a higher protocorm induction rate but required a longer time for protocorm initiation. According to Ravandi, Rezanejad & Dehghan, (2014) , the ability to regenerate shoots from callus or organogenesis in induced polyploid explants was significantly reduced. Tsukaya (2008) reported that Arabidopsis thaliana with more than eight sets of homologous chromosomes will display high ploidy syndrome, exhibiting contrasting effects by producing bigger cell in volume but smaller leaves.…”

Section: Discussionmentioning

confidence: 99%

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In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment

Eng

Ling³

2021

PeerJ

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Polyploidization has played a crucial role in plant breeding and crop improvement. However, studies on the polyploidization of tropical tree species are still very scarce in this region. This paper described the in vitro induction and identification of polyploid plants of Neolamarckia cadamba by colchicine treatment. N. cadamba belongs to the Rubiaceae family is a natural tetraploid plant with 44 chromosomes (2n = 4x = 44). Nodal segments were treated with colchicine (0.1%, 0.3% and 0.5%) for 24 h and 48 h before transferring to shoot regeneration medium. Flow cytometry (FCM) and chromosome count were employed to determine the ploidy level and chromosome number of the regenerants, respectively. Of 180 colchicine-treated nodal segments, 39, 14 and 22 were tetraploids, mixoploids and octoploids, respectively. The highest percentage of polyploidization (20% octoploids; 6.7% mixoploids) was observed after treated with 0.3% colchicine for 48 h. The DNA content of tetraploid (4C) and octoploid (8C) was 2.59 ± 0.09 pg and 5.35 ± 0.24 pg, respectively. Mixoploid plants are made up of mixed tetraploid and octoploid cells. Chromosome count confirmed that tetraploid cell has 44 chromosomes and colchicine-induced octoploid cell has 88 chromosomes. Both octoploids and mixoploids grew slower than tetraploids under in vitro conditions. Morphological characterizations showed that mixoploid and octoploid leaves had thicker leaf blades, thicker midrib, bigger stomata size, lower stomata density, higher SPAD value and smaller pith layer than tetraploids. This indicates that polyploidization has changed and resulted in traits that are predicted to increase photosynthetic capacity of N. cadamba. These novel polyploid plants could be valuable resources for advanced N. cadamba breeding programs to produce improved clones for planted forest development.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment

Eng

Ling³

2021

PeerJ

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“…Therefore, enhanced synthesis of phytochemical compounds through artificial chromosome doubling has been intensively investigated. Autotetraploid plants of Cichorium intybus accumulated higher contents of phenolic compounds than control plants [57]. In colchicine-induced polyploid Artemisia annua, tetraploid plants have increased artemisinin contents compared with that of diploid plants [58].…”

Section: Discussionmentioning

confidence: 94%

Comparative Analysis of the Transcriptome and Metabolome in Leaves of Diploid and Tetraploid Fagopyrum tataricum

Shi,

Qi,

Lin

et al. 2023

Phyton

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Tartary buckwheat (Fagopyrum tataricum) is a dual-purpose medicinal and food crop grown for its high contents of functional compounds and abundant nutrients. Although studies have shown the differences of total flavonoid content in Tartary buckwheat at different ploidy levels, the composition of flavonoid and its regulatory mechanisms are largely unknown. In this study, the leaf metabolome and transcriptome of diploid and tetraploid accessions of Tartary buckwheat were analyzed to gain insight into the impact of polyploidization on comparative secondary metabolite composition and molecular regulatory mechanism. Based on a widely targeted metabolomics analysis, a total of 792 metabolites were identified, including 127 flavonoids. The accumulation of 127 metabolites and expression of 3871 genes differed significantly between diploid and tetraploid Tartary buckwheat. Integrated metabolomics and transcriptome analysis revealed that chromosome doubling up-regulated the expression of upstream genes in the flavonoid biosynthesis pathway to promote the accumulation of flavonoids. The present results contribute to elucidation of the molecular mechanism of phenotypic variation associated with polyploidy in Tartary buckwheat. The findings provide a reference for further studies on phenotypic traits in polyploid Tartary buckwheat, the cloning of crucial regulatory genes, and utilization of genetic engineering technologies in the breeding of Tartary buckwheat.

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“…To name only a few, Ginger tuber can be processed into soy sauce to be accessary food, and chrysanthemum is popular table vegetable in China. In addition, many herbs of Compositae becomes healthy drink tea or herbal cuisine such as Cichorium intybus L., Artemisia annua L., Chamomilla recutita L. and that polyploidy in all the three health medical herbs were reported concerning morphological, physiological, cytological and phytochemical studies [129][130][131][132][133]. For instance, level of the total phenolic and chlorogenic acid content in the leaves of tetraploid Cichorium intybus was reported significantly enhanced [129].…”

Section: Artificial Polyploidy Induction In Medicinal Herbs Of Compos...mentioning

confidence: 99%

Advance in Cell Cultures, Cell Fusion and Cell Splitting of Medicinal and Edible Plant

Shi,

Wang

2023

Preprint

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With worsening environmental degradation and increasing international demand for human health, plant cell based technology will be more important. The aim of this review is to give an overview regarding key points of current progress and development trend of nature medical and edible plants from the framework of whole plant cell engineering including cell cultures, cell fusion and cell splitting derived biotechnology. Here we sum up three main application trends of medical plants cell cultures covering industrial output, technology upgrading and potential resource development. We also provide classification and typification insights to summarize the development of somatic hybridization in cereal, vegetables crops and other medicinal and edible plants as well as application of artificial polyploidy induction in major medicinal herbs. Development medicinal and edible homologous plants based on modern plant cell engineering will bring us a promising future.

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In vitro regeneration ability of diploid and autotetraploid plants of Cichorium intybus L.

Abstract: 37 Polyploidy has played a significant role in the evolutionary

Cited by 9 publications

References 29 publications

In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment

In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment

Comparative Analysis of the Transcriptome and Metabolome in Leaves of Diploid and Tetraploid Fagopyrum tataricum

Advance in Cell Cultures, Cell Fusion and Cell Splitting of Medicinal and Edible Plant

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