Nuclear factor of activated T cells and YY1 combine to repress IL-5 expression in a human T-cell line

SATB1 dictates expression of multiple genes including IL-5 involved in human T helper cell differentiation

“…45 The 5Ј flanking region of the human IL-5 gene has been reported to interact with many transcription factors, including GATA3, NFAT, YY1, and glucocorticoid receptor, which play important roles in IL-5 expression. 35,37,46 Our in vitro binding assay demonstrated that SATB1 binds 4 sites on the human IL-5 promoter. The SBSs S1 and S2 map to previously identified negative regulatory elements, 47,48 whereas S4 is located in a region shown to function as a positive element.…”

T-helper cell type-2 regulation in allergic disease

“…YY-1 can interact with a diverse array of other co-factors including histone-modifying enzymes [207], and one possibility is that YY-1 recruits these enzymes to the IL-4 locus. YY-1 has been shown to repress the IL-5 promoter in T-cell lines [208], and can also bind to the IFN-c promoter [209]. The ability of YY-1 to regulate IFN-c promoter activity is controversial, with both enhancing and repressing effects shown in different experiments [209,210].…”

HDAC inhibitors TSA and sodium butyrate enhanced the human IL-5 expression by altering histone acetylation status at its promoter region

“…IL-5 promoter contains three responsive elements (RE) and a distal regulatory region, among which RE I (−80 bp to −45 bp) and RE II (−123 bp to −92 bp) regions are activating elements while RE III (−170 bp to −130 bp) and the distal regulatory region (−460 bp to −230 bp) are repressive elements [15,16]. A series of previous studies have demonstrated that several transcription factors, such as C/EBP␤, GATA3, NFAT, YY1, AP1 and Ets1, are able to bind onto these regions and significantly regulate IL-5 transcription [17][18][19][20][21][22][23].…”