Nuclear DNA contents of Pisum genotypes grown in vivo and in vitro

Schweizer, Dieter; Davies, D. R.

doi:10.1007/bf00385470

Cited by 12 publications

(5 citation statements)

References 13 publications

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“…Schweizer and Davies (1972) found a statistically significant 1.07-fold maximum difference between four Pisum sativum accessions, which is by and large compatible with our observations. Compared with the much larger difference between root samples found in the same investigation (see above) this variation appears inconspicuous.…”

Section: Discussionsupporting

confidence: 94%

“…There is also a vague impression that quick-and -slow-growing meristems, and main and side roots give slightly different Feulgen values; however, our search for reproducible differences yielded mostly nonsignificant results. These differences, up to 4%, were smaller than the 13% reported by Schweizer and Davies (1972) between main roots from seedlings and laterals on detached main roots cultured in vitro. Moreover, the differences found between telophases and prophases of about 3% show that variation between samples in that size order must not be taken too ples in the present study.…”

Section: Discussioncontrasting

confidence: 75%

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Genome size variation in Pisum sativum

Greilhuber¹,

Ebert²

1994

Genome

173

113

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Pisum sativum L. is one of the plant species where infraspecific genome size variation, up to 1.29-fold between cultivars, has been reported. The present investigation deals with a Feulgen cytophotometric analysis of this phenomenon in 25 wild accessions, landraces, and cultivars of widely different geographic origin. Differences between accessions were maximally 1.054-fold in single experiments but proved to be nonreproducible upon repeated measurements. Seedlings of the same accession often differed significantly, up to 1.056-fold, but values from root and shoot tips in one individual were not significantly correlated, indicating the absence of true genome size variation between plants. Upon calibration against Allium cepa a 1C value of 4.42 pg is estimated for Pisum sativum. Altogether the data suggest that, contrary to the divergence in the literature data and recent reports on DNA content variation, the pea has a stable genome size.

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Section: Discussionsupporting

confidence: 94%

Section: Discussioncontrasting

confidence: 75%

Genome size variation in Pisum sativum

Greilhuber¹,

Ebert²

1994

Genome

173

113

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“…For instance, A bbott (1971) claim ed th a t the D N A content of cells of excised pea roots cultured in vitro was only 27 % of th a t in cells of attached control roots. Schweizer & Davies (1972) repeated A bbott's experiments and found no evidence to support his claim, although they did find significant differences in 4(7 DNA contents of up to 7 % between cultivated varieties of Pisum sativum and P. sativum ssp. abyssinicum.…”

Section: Intraspecific Variation In D N a Amount Per Genomementioning

confidence: 84%

Nuclear DNA amounts in angiosperms

Bennett

Smith²

1976

Phil. Trans. R. Soc. Lond. B

1,392

454

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The number of angiosperm species for which nuclear DNA amount estimates have been made has nearly trebled since the last collected lists of such values were published, and therefore, publication of a more comprehensive list is over due. This paper lists absolute nuclear DNA amounts for 753 angiosperm species. The dats were assembled primarily for reference purposes, and so the species are listed in alphabetical order, as this was felt to be more helpful to cyto- and biochemists whom, it is anticipated, will be among its major users. The paper also reviews aspects of the history, nomenclature, methods, accuracy and problems of nuclear DNA estimation in angiosperms. No attempt is made to reconsider those aspects of nuclear DNA estimation which have been fully revised previously, although the bibliography of such aspects is given. Instead, the paper is intended as a source of basic information regarding the terminology, practice and limitations of nuclear DNA estimation, especially by Feulgen microdensitometry, as currently practiced.

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“…Grant (1969) used Feulgen cytophotometry to determine DNA contents and ploidy levels in Betula species, and Bennett (1971) has presented extensive data on the correlation of DNA content with growth habit for herbaceous flowering plants. Using the same techniques, Schweizer and Davies ( 1972) and Woodard and Swift (1964) determined DNA content of individual chromosomes in Trillium erectum. The method, with slight modifications, has been successfully applied to animal tissues (Hughes-Schrader, 1953;Evans, 1956;John and Hewitt, 1966;Beamish, Merriless, and Crossman, 1971;James, 1972).…”

mentioning

confidence: 99%

DNA Content of Seven Species of Astereae and its Significance to Theories of Chromosome Evolution in the Tribe

Stucky

Jackson

1975

American Journal of Botany

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Relative amounts of nuclear DNA were determined in root tip cells of seven species of Astereae: Aster hydrophilus Greene, A. oblongijolius Nutt., A. riparius H.B.K., Machaeranthera boltoniae (Greene) Turner and Horne, M. brevilingulata (Sch-Bip.) Turner and Horne, M. parviilora Gray, and M. tenuis (S. Wats.) Turner and Horne. The results show that A. hydrophilus and M. brevilingulata, with a chromosome number of n = 9, have less nuclear DNA than other closely related species which are either n =4 or n =5. Cytological analyses of meiosis in the intergeneric hybrid M. parviflora X A. hydrophilus showed cells with two or more small chromosomes of the latter species pairing with single large chromosomes of the former. Pachytene cells of the hybrids M. parvijlora X A. hydrophilus, M. parviflora X A. riparius, and M. boltoniae X M. tenuis showed some unpaired chromosome segments. The significance of these results to chromosome evolution in the tribe Astereae is discussed.

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Nuclear DNA contents of Pisum genotypes grown in vivo and in vitro

Cited by 12 publications

References 13 publications

Genome size variation in Pisum sativum

Genome size variation in Pisum sativum

Nuclear DNA amounts in angiosperms

DNA Content of Seven Species of Astereae and its Significance to Theories of Chromosome Evolution in the Tribe

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