Nuclear and cytoplasmic adenovirus RNA. Differences between 5′-termini of messenger and non-messenger transcripts

McGuire, Patricia; Piatak, Michael; Hodge, L. D.

doi:10.1016/0022-2836(76)90154-6

Cited by 20 publications

(4 citation statements)

References 31 publications

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“…The two species, VA RNA, and VA RNA,, (also known as 5.5S and 5.2S RNA), occur in a major fonn about 160 nucleotides in length and in an array of minor forms differing at both their 5' and 3' ends (5,6,16,24,43). Again in contrast to mRNA, the VA RNAs seem to be free of internal and terminal modifications such as methylation and capping, apart from a 5'-terminal polyphosphate vestige of RNA chain initiation (6,25,34,36,43). The genes that code for the VA RNAs are closely spaced, lying at or near the left (5') end of the main block of late structural genes, and are also transcribed in a rightward direction (22,24,32).…”

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confidence: 99%

Virus-associated RNAs of naturally occurring strains and variants of group C adenoviruses

Mathews

Grodzicker

1981

J Virol

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We compared the sequences of the virus-associated (VA) RNAs of group C adenoviruses, serotypes 1, 2, 5, and 6, and of three variants of adenovirus type 2 (Ad2) selected for loss of the BamHI restriction site in the VA RNA, gene. In the naturally occurring strains, VA RNA, exists in two forms which differ by two nucleotides: one form is found in Ad2 and Ad6, and the other is found in Adl and Ad5. There are three sites of variation in VA RNA,,, the Adl, Ad2, and Ad5 forms each differing from Ad6 VA RNA5 at one of the positions. One of the selected variants has a four-base duplication within the BantHI cleavage site, whereas the two others have acquired a VA RNA, sequence indistinguishable from that of Ad5. The findings are interpreted in terms of the secondary structures of the VA RNAs and the interrelationships among the viruses.

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confidence: 99%

Virus-associated RNAs of naturally occurring strains and variants of group C adenoviruses

Mathews

Grodzicker

1981

J Virol

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“…After digestion with a mixture of , T2, and pancreatic A ribonucleases, both mRNA pools yielded radioactive products that migrated during ionophoresis as two peaks on DEAE paper with mobilities slower than a marker with a net charge of -4 (Figure 1, panels a and b). Previous results have established that it is to this region of the DEAE paper that the 5' terminus of reovirus (m7GpppGmpCp) migrates under the ionophoretic conditions used here (McGuire et al, 1976). In addition to the two broad peaks in the S'-terminal region, digests of poly(A)-containing mRNA also contained radioactivity that migrated as a single sharp peak to the mononucleotide region of the paper (Figure 1, panel b).…”

Section: Resultsmentioning

confidence: 67%

“…-uninfected mammalian cells (Adams and Cory, 1975; Cory and Adams, 1975;Furuichi et al, 1975b;Lavi and Shatkin, 1975;McGuire et al, 1976;Rose, 1975; Shatkin, personal communication; Wei et al, 1975a). One example of the heterogeneity of modified sequences found at the 5' termini of cellular mRNA has appeared recently.…”

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Histone messenger RNA from HeLa cells: evidence for modified 5' termini

Stein¹,

Stein²,

McGuire³

1977

Biochemistry

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The distribution of [3H]methyl radioactivity in cytoplasmic histone mRNA, isolated during the DNA synthetic (S) phase of the HeLa S3 cell cycle, has been investigated. Evidence is presented that approximately 30% of the radioactivity is in m7GpppXmpYp oligonucleotides, where Xm represents 2'-O-methylated adenosine and guanosine with a molar ratio of 4:1, respectively. The remainder of the radioactivity is present as m7GpppCmpYmpZp oligonucleotides, where Xm is again 2'-O-methylated adenosine and guanosine (4:1) and where ym represents 2'-O-methylated adenosine, guanosine, cytidine, and uridine with ratios of 2:1:1:1, respectively. While 48.6% of the [3H]methyl radioactivity was present as N6-methyladenosine in poly(adenylic acid)-terminated mRNA from S-phase cells, no evidence for N6-methyladenosine was found in histone mRNA. It thus appears that histone mRNA which lacks 3'-terminal poly(adenylic acid) sequences and functions on cytoplasmic polyribosomes during a limited protion of the cell cycle is capped but lacks internal-modified nucleosides.

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“…Whereas most if not all cellular mRNAs are capped (19,22), not all hnRNAs bear the blocked terminus (23,24); at least 50% of hnRNA molecules are capped in mouse L cells (25). It thus appears that capped hnRNA molecules serve as precursors to mRNA, and hnRNA molecules without caps probably do not give rise to mRNAs (26). The fate of nuclear transcripts may therefore be determined by their being capped or not capped.…”

Section: Introductionmentioning

confidence: 99%

The 5′ terminal capping of heterogeneous nuclear RNA at different embryonic stages of the sea urchin

Nemer¹,

Surrey²,

Ginzburg³

et al. 1979

Nucl Acids Res

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5' Terminal cap structures of hnRNA have been characterized and the extent of capping determined as a function of embryonic development. Sea urchin embryo hnRNA contains only the type-I cap, m7GpppNmpNp, with the type-2 cap, which has a 2'-0-methylated subpenultimate nucleotide, being associated only with stable small nuclear RNAs. These cap 2-containing RNAs are synthesized at a rate of approximately 70 molecules min-1 nucleus-I compared to approximately 1000 molecules for hnRNA cap 1. Approximately 70% of nuclear cap 1 is associated with > 15S RNA in denaturing solvent, but under non-denaturing conditions the percentage is much higher. Cap 1 in low and high molecular weight nuclear RNA have the same kinetics of methyl labeling. Thus all cap 1 structures may belong to a single class either covalent or H-bonded to high molecular weight RNA. hnRNA > 15S is 35% capped; however, adding caps in < 15S RNA gives an estimate of 50% capping for total hnRNA. In development from early blastula to late gastrula, there is little if any change in the extent of capping of hnRNA. These results coupled with others indicate that the fraction of hnRNA molecules serving as precursor to mRNA does not change quantitatively during embryonic development.

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Nuclear and cytoplasmic adenovirus RNA. Differences between 5′-termini of messenger and non-messenger transcripts

Cited by 20 publications

References 31 publications

Virus-associated RNAs of naturally occurring strains and variants of group C adenoviruses

Virus-associated RNAs of naturally occurring strains and variants of group C adenoviruses

Histone messenger RNA from HeLa cells: evidence for modified 5' termini

The 5′ terminal capping of heterogeneous nuclear RNA at different embryonic stages of the sea urchin

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