NTRC Plays a Crucial Role in Starch Metabolism, Redox Balance, and Tomato Fruit Growth

Hou, Liang‐Yu; Ehrlich, Matthias; Thormählen, Ina; Lehmann, Martin; Krahnert, Ina; Obata, Toshihiro; Cejudo, Francisco Javier; Fernie, Alisdair R.; Geigenberger, Peter

doi:10.1104/pp.19.00911

Cited by 20 publications

(19 citation statements)

References 87 publications

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“…The detection of metabolite followed the method published before [ 54 , 55 , 56 ]. The details of sample preparation and condition were also described in the previous publication [ 57 ]. The results were analyzed using ChromaTOF 4.5 and TagFinder 4.1 software [ 58 ].…”

Section: Methodsmentioning

confidence: 99%

Thioredoxin h2 and o1 Show Different Subcellular Localizations and Redox-Active Functions, and Are Extrachloroplastic Factors Influencing Photosynthetic Performance in Fluctuating Light

2021

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Arabidopsis contains eight different h-type thioredoxins (Trx) being distributed in different cell organelles. Although Trx h2 is deemed to be confined to mitochondria, its subcellular localization and function are discussed controversially. Here, cell fractionation studies were used to clarify this question, showing Trx h2 protein to be exclusively localized in microsomes rather than mitochondria. Furthermore, Arabidopsis trxo1, trxh2 and trxo1h2 mutants were analyzed to compare the role of Trx h2 with mitochondrial Trx o1. Under medium light, trxo1 and trxo1h2 showed impaired growth, while trxh2 was similar to wild type. In line with this, trxo1 and trxo1h2 clustered differently from wild type with respect to nocturnal metabolite profiles, revealing a decrease in ascorbate and glutathione redox states. Under fluctuating light, these genotypic differences were attenuated. Instead, the trxo1h2 double mutant showed an improved NADPH redox balance, compared to wild type, accompanied by increased photosynthetic efficiency, specifically in the high-light phases. Conclusively, Trx h2 and Trx o1 are differentially localized in microsomes and mitochondria, respectively, which is associated with different redox-active functions and effects on plant growth in constant light, while there is a joint role of both Trxs in regulating NADPH redox balance and photosynthetic performance in fluctuating light.

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Section: Methodsmentioning

confidence: 99%

Thioredoxin h2 and o1 Show Different Subcellular Localizations and Redox-Active Functions, and Are Extrachloroplastic Factors Influencing Photosynthetic Performance in Fluctuating Light

2021

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“…In general, the conversion of storage starch to soluble sugars is required for post‐harvest ripening in climacteric fruits (Graham et al, 1994; Saquet and Almeida, 2017; Hou et al, 2019). However, fig fruit accumulates less starch compared to most climacteric fruits (Figure 1), and having levels similar to those of strawberry and peach fruits (Vizzotto et al, 1996; Jia et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Fig fruit ripening is regulated by the interaction between ethylene and abscisic acid

Qiao

Zhang

Zhun

et al. 2021

JIPB

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Fleshy fruit ripening is typically regulated by ethylene in climacteric fruits and abscisic acid (ABA) in non‐climacteric fruits. Common fig (Ficus carica) shows a dual‐ripening mechanism, which is not fully understood. Here, we detected separate peaks of ethylene and ABA in fig fruits at the onset‐ and on‐ripening stages, in conjunction with a sharp rise in glucose and fructose contents. In a newly‐designed split‐fruit system, exogenous ethylene failed to rescue fluridone‐inhibited fruit ripening, whereas exogenous ABA rescued 2‐amino‐ethoxy‐vinyl glycine (AVG)‐inhibited fruit ripening. Transcriptome analysis revealed changes in the expression of genes key to both ABA and ethylene biosynthesis and perception during fig fruit ripening. At the de‐greening stage, downregulation of FcACO2 or FcPYL8 retarded ripening, but downregulation of FcETR1/2 did not; unexpectedly, downregulation of FcAAO3 promoted ripening, but it inhibited ripening only before the de‐greening stage. Furthermore, we detected an increase in ethylene emissions in the FcAAO3‐RNAi ripening fruit and a decrease in ABA levels in the FcACO2‐RNAi unripening fruit. Importantly, FcPYL8 can bind to ABA, suggesting that it functions as an ABA receptor. Our findings support the hypothesis that ethylene regulates the fig fruit ripening in an ABA‐dependent manner. We propose a model for the role of the ABA–ethylene interaction in climacteric/non‐climacteric processes.

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“…regulated by redox-dependent ADP-glucose pyrophosphorylase (AGPase) activation (Hou et al, 2019). Hence, we sought to understand the role of Ta2CP in controlling starch accumulation.…”

Section: Ta2cp-mediated Effect On Starch Biosynthesismentioning

confidence: 99%

Wheat 2‐Cys peroxiredoxin plays a dual role in chlorophyll biosynthesis and adaptation to high temperature

et al. 2021

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The molecular mechanism of high-temperature stress (HTS) response, in plants, has so far been investigated using transcriptomics, while the dynamics of HTS-responsive proteome remain unexplored. We examined the adaptive responses of the resilient wheat cultivar 'Unnat Halna' and dissected the HTS-responsive proteome landscape. This led to the identification of 55 HTS-responsive proteins (HRPs), which are predominantly involved in metabolism and defense pathways. Interestingly, HRPs included a 2-cysteine peroxiredoxin (2CP), designated Ta2CP, presumably involved in stress perception and adaptation. Complementation of Ta2CP in yeast and heterologous expression in Arabidopsis demonstrated its role in thermotolerance. Both Ta2CP silencing and overexpression inferred the involvement of Ta2CP in plant growth and chlorophyll biosynthesis. We demonstrated that Ta2CP interacts with protochlorophyllide reductase b, TaPORB. Reduced TaPORB expression was found in Ta2cp-silenced plants, while upregulation was observed in Ta2CP-overexpressed plants. Furthermore, the downregulation of Ta2CP in Taporb-silenced plants and reduction of protochlorophyllide in Ta2cp-silenced plants suggested the key role of Ta2CP in chlorophyll metabolism. Additionally, the transcript levels of AGPase1 and starch were increased in Ta2cp-silenced plants. More significantly, HTS-treated Ta2cp-silenced plants showed adaptive responses despite increased reactive oxygen species and peroxide concentrations, which might help in rapid induction of high-temperature acclimation.

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NTRC Plays a Crucial Role in Starch Metabolism, Redox Balance, and Tomato Fruit Growth

Cited by 20 publications

References 87 publications

Thioredoxin h2 and o1 Show Different Subcellular Localizations and Redox-Active Functions, and Are Extrachloroplastic Factors Influencing Photosynthetic Performance in Fluctuating Light

Thioredoxin h2 and o1 Show Different Subcellular Localizations and Redox-Active Functions, and Are Extrachloroplastic Factors Influencing Photosynthetic Performance in Fluctuating Light

Fig fruit ripening is regulated by the interaction between ethylene and abscisic acid

Wheat 2‐Cys peroxiredoxin plays a dual role in chlorophyll biosynthesis and adaptation to high temperature

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