Wheat 2‐Cys peroxiredoxin plays a dual role in chlorophyll biosynthesis and adaptation to high temperature

Abstract

The molecular mechanism of high-temperature stress (HTS) response, in plants, has so far been investigated using transcriptomics, while the dynamics of HTS-responsive proteome remain unexplored. We examined the adaptive responses of the resilient wheat cultivar 'Unnat Halna' and dissected the HTS-responsive proteome landscape. This led to the identification of 55 HTS-responsive proteins (HRPs), which are predominantly involved in metabolism and defense pathways. Interestingly, HRPs included a 2-cysteine peroxiredoxin (2CP), designated Ta2CP, presumably involved in stress perception and adaptation. Complementation of Ta2CP in yeast and heterologous expression in Arabidopsis demonstrated its role in thermotolerance. Both Ta2CP silencing and overexpression inferred the involvement of Ta2CP in plant growth and chlorophyll biosynthesis. We demonstrated that Ta2CP interacts with protochlorophyllide reductase b, TaPORB. Reduced TaPORB expression was found in Ta2cp-silenced plants, while upregulation was observed in Ta2CP-overexpressed plants. Furthermore, the downregulation of Ta2CP in Taporb-silenced plants and reduction of protochlorophyllide in Ta2cp-silenced plants suggested the key role of Ta2CP in chlorophyll metabolism. Additionally, the transcript levels of AGPase1 and starch were increased in Ta2cp-silenced plants. More significantly, HTS-treated Ta2cp-silenced plants showed adaptive responses despite increased reactive oxygen species and peroxide concentrations, which might help in rapid induction of high-temperature acclimation.

Monitoring cellular redox dynamics using newly developed BRET-based redox sensor proteins

“…Furthermore, high- and low-temperature stress in higher plants generates ROS ( 44 , 45 ). In wheat, for example, high-temperature stress conditions induce the expression of 2-Cys Prx, a ROS scavenger ( 46 ). Therefore, ROBINc might be useful for monitoring the redox status in various photosynthetic organisms.…”

Genetic and molecular mechanisms underlying root architecture and function under heat stress—A hidden story

“…Apart from these well‐characterized genes, many other genes coding for kinases, transcription factors, HSFs and HSPs, are known to regulate either heat stress or root development. However, their molecular mechanisms underlying root development during heat stress needs in planta functional characterization (Dhakate et al, 2021; Mishra et al, 2020; Mishra, Shekhar, et al, 2021; Mittler et al, 2012; Ohama et al, 2017; Tiwari, Pandey, Singh, Yadav, et al, 2021). Investigations involving in planta overexpression of these genes indicated that a better root architecture provides superior support to the overall plant stature.…”

Effective Use of Water in Crop Plants in Dryland Agriculture: Implications of Reactive Oxygen Species and Antioxidative System

“… Huang et al (2020) reported that arbuscular mycorrhizal fungi (AMF) could utilize the MAPKs signaling pathway to enhance drought tolerance by using MAPK signaling as an intermediate pathway for interactions between AMF and their host apple plant. Besides, the extensive role of MAPKs, other kinases such as histidine kinases, CDPKs, leucine-rich repeat-receptor-like kinases, and serine-threonine protein kinases are also at the center of stress tolerance ( Mishra et al, 2021a ; Tiwari et al, 2021a , b ). Early leaf development is regulated by genes-encoding OsSIK2 , a protein kinase located in rice leaf and sheath plasma membrane.…”

“…ROS production is directly related to stress in plants and leads to membrane damage and subsequent stress signaling ( Mishra et al, 2017 , 2018 ). ROS production takes place in the reaction centers of photosystem I (PSI) and PSII in chloroplast thylakoids ( Foyer, 2018 ; Mishra et al, 2021a ). ROS species formation is a result of the direct transfer of the excitation energy from chlorophyll to produce singlet oxygen, or by oxygen reduction in the Mehler reaction ( Foyer, 2018 ).…”

“…Due to the lack of an enzymatic mechanism, it is hard to eliminate this highly reactive radical, and its enhanced accumulation leads to cell death ( Das and Roychoudhury, 2014 ). The enzymes, namely catalase (CAT), and different types of peroxidases can scavenge H 2 O 2 from the cell ( Mishra et al, 2021a ). Similar to the enzymatic antioxidant defense system, the plant also possesses non-enzymatic antioxidants like carotenoids, flavonoids, and ascorbate, which are synthesized in response to excess light stress and complement the antioxidant enzymes in mitigating ROS ( Hatier and Gould, 2008 ; Agati et al, 2009 , 2011 ).…”