NTA Directed Protein Nanopatterning on DNA Origami Nanoconstructs

Abstract

Precisely patterning proteins and other molecules at the nanoscale is crucial to future biosensing and optoelectronic applications. One- and two-dimensional DNA nanoconstructs have proven to be useful scaffolds for nanopatterning. This paper demonstrates the application of nitrilotriacetic acid (NTA) forming chelate complexes to localize histidine (His) tagged proteins via Ni(2+) ions onto DNA based structures. Particularly, enhanced green fluorescent protein (EGFP) was directed to specific surface locations on a designed DNA Origami nanoconstruct, and the resulting EGFP nanopattern was visualized using atomic force microscopy (AFM).

DNA Origami as a Nanoscopic Ruler for Super‐Resolution Microscopy

“…[18] Recent advances in the origami technique [19] will enable our concept to be extended to three dimensions and to super-resolution techniques requiring photoswitchable fluorescent proteins. [20] Single-molecule methods can thus cover the full critical length scale from a few nanometers using fluorescenceresonance energy transfer (FRET) to the length scales of conventional optical microscopy. Such advances are also crucial for the study of dynamic processes such as diffusive or directed transport occurring on DNA-based nanostructures.…”

Catenoid Stability with a Free Contact Line

“…Recently, interests have grown into areas such as elastocapillarity [7][8][9][10], contact-drop dispensing [11] with applications to scanning-probe lithography [12] and micromachined fountain-pen techniques [13]. Molecular-resolution surface patterning provides new opportunities for advanced tissue engineering [14], DNA self-assembled nanoconstructs [15], and highly sensitive protein chips [16].…”

DNA origami deposition on native and passivated molybdenum disulfide substrates

“…In contrast, several materials have been found that enable the deposition of DNA origami structures while maintaining their structural integrity. These materials include mica [12], silicon dioxide [13], gold [14], and graphene oxide [2]. The ideal substrate surface must be atomically smooth to enable optimal patterning and imaging through atomic force microscopy (AFM) because the origami structures are very thin and conformal.…”