DNA origami deposition on native and passivated molybdenum disulfide substrates

Abstract

Maintaining the structural fidelity of DNA origami structures on substrates is a prerequisite for the successful fabrication of hybrid DNA origami/semiconductor-based biomedical sensor devices. Molybdenum disulfide (MoS 2 ) is an ideal substrate for such future sensors due to its exceptional electrical, mechanical and structural properties. In this work, we performed the first investigations into the interaction of DNA origami with the MoS 2 surface. In contrast to the structure-preserving interaction of DNA origami with mica, another atomically flat surface, it was observed that DNA origami structures rapidly lose their structural integrity upon interaction with MoS 2 . In a further series of studies, pyrene and 1-pyrenemethylamine, were evaluated as surface modifications which might mitigate this effect. While both species were found to form adsorption layers on MoS 2 via physisorption, 1-pyrenemethylamine serves as a better protective agent and preserves the structures for significantly longer times. These findings will be beneficial for the fabrication of future DNA origami/MoS 2 hybrid electronic structures. 501

One-step large-scale deposition of salt-free DNA origami nanostructures

“…This feature could be exploited in a roll-to-roll production line, where large surfaces can be coated with homogeneous films. The method has potential implementations for example in creating large-scale DNA chips and self-assembly -based biosensors on specific substrates 54 . Further, metallisation of DNA nanoshapes 49 55 might enable fabrication of large films with attractive plasmonic or electrical properties.…”

Two‐Dimensional Transition Metal Dichalcogenides in Biosystems

“…2D MoS 2 has been implemented for assessing cell viabilities based on exchanged alkali ions via protein nanopores embedded in cells' lipid bilayers, a process which is empowered by the intrinsic voltage across such nanopores. They have also been implemented in realising DNA‐origami concepts . Another application is in DNA sequencing, where nanopores formed in 2D TMDCs offer a signal‐to‐noise ratio almost one order of magnitude superior to that of graphene .…”

Optical fingerprints and electron transport properties of DNA bases adsorbed on monolayer MoS2

“…the photoluminescence spectra (PL) of MoS 2 reveals the possibility of optical biosensors 19 ; modulation in PL due to the presence of DNA base could serve as a fingerprint for selective optical biosensing 19 ; nano-pore in pristine MoS 2 results in fast detection of DNA bases 17 ; MoS 2 based biomedical sensor shows promise to detect DNA origami, tumor necrosis factor-alpha (TNF-a) molecules etc. [21][22][23][24][25] .…”