NQO1 activity in the main and the accessory olfactory systems correlates with the zonal topography of projection maps

Gussing, Fredrik; Bohm, Staffan

doi:10.1111/j.0953-816x.2004.03331.x

Cited by 87 publications

(82 citation statements)

References 53 publications

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“…S1). To distinguish expression in dorsal versus ventral OE domains, we used a probe for Nqo1 (NAD(P)H dehydrogenase, quinone 1), which is selectively expressed in the dorsal domain (26).…”

Section: Resultsmentioning

confidence: 99%

Olfactory receptor genes expressed in distinct lineages are sequestered in different nuclear compartments

Yoon

Ragoczy

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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The olfactory system translates a vast array of volatile chemicals into diverse odor perceptions and innate behaviors. Odor detection in the mouse nose is mediated by 1,000 different odorant receptors (ORs) and 14 trace amine-associated receptors (TAARs). ORs are used in a combinatorial manner to encode the unique identities of myriad odorants. However, some TAARs appear to be linked to innate responses, raising questions about regulatory mechanisms that might segregate OR and TAAR expression in appropriate subsets of olfactory sensory neurons (OSNs). Here, we report that OSNs that express TAARs comprise at least two subsets that are biased to express TAARs rather than ORs. The two subsets are further biased in Taar gene choice and their distribution within the sensory epithelium, with each subset preferentially expressing a subgroup of Taar genes within a particular spatial domain in the epithelium. Our studies reveal one mechanism that may regulate the segregation of Olfr (OR) and Taar expression in different OSNs: the sequestration of Olfr and Taar genes in different nuclear compartments. Although most Olfr genes colocalize near large central heterochromatin aggregates in the OSN nucleus, Taar genes are located primarily at the nuclear periphery, coincident with a thin rim of heterochromatin. Taar-expressing OSNs show a shift of one Taar allele away from the nuclear periphery. Furthermore, examination of hemizygous mice with a single Taar allele suggests that the activation of a Taar gene is accompanied by an escape from the peripheral repressive heterochromatin environment to a more permissive interior chromatin environment. olfactory receptor genes | Taar genes | nuclear organization

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“…S1). To distinguish expression in dorsal versus ventral OE domains, we used a probe for Nqo1 (NAD(P)H dehydrogenase, quinone 1), which is selectively expressed in the dorsal domain (26).…”

Section: Resultsmentioning

confidence: 99%

Olfactory receptor genes expressed in distinct lineages are sequestered in different nuclear compartments

Yoon

Ragoczy

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…OE regions expressing high levels of robo-2 overlap with the region containing NQO-1-expressing OSNs, which are located in zone I of the OE according to the nomenclature defined by Sullivan et al (1996) (Fig. 1C,D) (Gussing and Bohm, 2004). Robo-2 expression also partially overlaps regions of the OE containing OCAM-expressing OSNs that define zones II-IV of the OE (Fig.…”

Section: Robo-2 Expression By Olfactory Sensory Neuronsmentioning

confidence: 95%

Requirement for Slit-1 and Robo-2 in Zonal Segregation of Olfactory Sensory Neuron Axons in the Main Olfactory Bulb

Cho¹,

Lépine²,

Andrews³

et al. 2007

J. Neurosci.

111

125

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The formation of precise stereotypic connections in sensory systems is critical for the ability to detect and process signals from the environment. In the olfactory system, olfactory sensory neurons (OSNs) project axons to spatially defined glomeruli within the olfactory bulb (OB). A spatial relationship exists between the location of OSNs within the olfactory epithelium (OE) and their glomerular targets along the dorsoventral axis in the OB. The molecular mechanisms underlying the zonal segregation of OSN axons along the dorsoventral axis of the OB are poorly understood. Using robo-2 Ϫ/Ϫ (roundabout) and slit-1 Ϫ/Ϫ mice, we examined the role of the Slit family of axon guidance cues in the targeting of OSN axons during development. We show that a subset of OSN axons that normally project to the dorsal region of the OB mistarget and form glomeruli in the ventral region in robo-2 Ϫ/Ϫ and slit-1 Ϫ/Ϫ mice. In addition, we show that the Slit receptor, Robo-2, is expressed in OSNs in a high dorsomedial to low ventrolateral gradient across the OE and that Slit-1 and Slit-3 are expressed in the ventral region of the OB. These results indicate that the dorsal-to-ventral segregation of OSN axons are not solely defined by the location of OSNs within the OE but also relies on axon guidance cues.

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“…One parameter that defines the axonal projection site is positional information in the OE. Antibody staining of zone-specific molecules showed a zone-to-zone correlation: OCAM-positive V zone OSNs project axons to the ventral (V) domain of the OB, whereas NQO-1-positive D zone OSNs project axons to the dorsal (D) domain of the OB (Alenius and Bohm 1997;Gussing and Bohm 2004) (Fig. 1B).…”

Section: Mechanisms Of Olfactory Map Formation In Mammals Topographicmentioning

confidence: 99%

Topographic Mapping--The Olfactory System

Imai¹,

Sakano²,

Vosshall³

2010

Cold Spring Harbor Perspectives in Biology

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Sensory systems must map accurate representations of the external world in the brain. Although the physical senses of touch and vision build topographic representations of the spatial coordinates of the body and the field of view, the chemical sense of olfaction maps discontinuous features of chemical space, comprising an extremely large number of possible odor stimuli. In both mammals and insects, olfactory circuits are wired according to the convergence of axons from sensory neurons expressing the same odorant receptor. Synapses are organized into distinctive spherical neuropils-the olfactory glomeruli-that connect sensory input with output neurons and local modulatory interneurons. Although there is a strong conservation of form in the olfactory maps of mammals and insects, they arise using divergent mechanisms. Olfactory glomeruli provide a unique solution to the problem of mapping discontinuous chemical space onto the brain.T he olfactory system detects airborne organic and inorganic chemicals that originate from plant and animal metabolites and environmental and industrial sources. Odors mediate both innate and learned behaviors such as attraction and aversion, governing decisions to eat, mate, attack or flee from aggressors and predators. A remarkable feature of the olfactory system is the extraordinary diversity of possible odor molecules that exist. Although accurate measurements can be made of the detection range of visual wavelengths or auditory frequencies in humans, there are no reliable estimates of the number of odorous molecules that exist on earth or those that can be detected by a given animal species (Gilbert 2008). Nevertheless, the number of possible odorants is thought to be high, in the range of many thousands. Beyond odor detection lies the problem of odor discrimination: how can chemicals with slightly different physical properties be discriminated and associated with distinct odor percepts?Experiments investigating the molecular logic of olfaction over the last 20 years have led to major insights into how animals solve the problem of detecting and discriminating a vast number of different odor stimuli (Axel 1995). Four organizational principles have emerged from this work. First, large numbers of distinct odorant receptor (OR) genes are dedicated

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NQO1 activity in the main and the accessory olfactory systems correlates with the zonal topography of projection maps

Cited by 87 publications

References 53 publications

Olfactory receptor genes expressed in distinct lineages are sequestered in different nuclear compartments

Olfactory receptor genes expressed in distinct lineages are sequestered in different nuclear compartments

Requirement for Slit-1 and Robo-2 in Zonal Segregation of Olfactory Sensory Neuron Axons in the Main Olfactory Bulb

Topographic Mapping--The Olfactory System

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