NpPDR1, a Pleiotropic Drug Resistance-Type ATP-Binding Cassette Transporter from Nicotiana plumbaginifolia, Plays a Major Role in Plant Pathogen Defense

Abstract

Nicotiana plumbaginifolia NpPDR1, a plasma membrane pleiotropic drug resistance-type ATP-binding cassette transporter formerly named NpABC1, has been suggested to transport the diterpene sclareol, an antifungal compound. However, direct evidence for a role of pleiotropic drug resistance transporters in the plant defense is still lacking. In situ immunolocalization and histochemical analysis using the gusA reporter gene showed that NpPDR1 was constitutively expressed in the whole root, in the leaf glandular trichomes, and in the flower petals. However, NpPDR1 expression was induced in the whole leaf following infection with the fungus Botrytis cinerea, and the bacteria Pseudomonas syringae pv tabaci, Pseudomonas fluorescens, and Pseudomonas marginalis pv marginalis, which do not induce a hypersensitive response in N. plumbaginifolia, whereas a weaker response was observed using P. syringae pv syringae, which does induce a hypersensitive response. Induced NpPDR1 expression was more associated with the jasmonic acid than the salicylic acid signaling pathway. These data suggest that NpPDR1 is involved in both constitutive and jasmonic acid-dependent induced defense. Transgenic plants in which NpPDR1 expression was prevented by RNA interference showed increased sensitivity to sclareol and reduced resistance to B. cinerea. These data show that NpPDR1 is involved in pathogen resistance and thus demonstrate a new role for the ATP-binding cassette transporter family.

ArabidopsisPEN3/PDR8, an ATP Binding Cassette Transporter, Contributes to Nonhost Resistance to Inappropriate Pathogens That Enter by Direct Penetration

“…Alternatively, this gene could be a novel marker for the stress experienced by pen3 plants. In spite of the expression responses, loss of PDR12 function in Arabidopsis or PDR1 function in N. plumbaginifolia did not lead to increased susceptibility to several fungal and bacterial pathogens (Campbell et al, 2003;Stukkens et al, 2005). However, N. plumbaginifolia plants lacking PDR1 were highly susceptible to the necrotroph B. cinerea (Stukkens et al, 2005).…”

“…In spite of the expression responses, loss of PDR12 function in Arabidopsis or PDR1 function in N. plumbaginifolia did not lead to increased susceptibility to several fungal and bacterial pathogens (Campbell et al, 2003;Stukkens et al, 2005). However, N. plumbaginifolia plants lacking PDR1 were highly susceptible to the necrotroph B. cinerea (Stukkens et al, 2005). With this result and the cloning of PEN3, PDR-type ABC transporters have been implicated in defense, adding a new role to the diverse repertoire of the PDR-like ABC transporter family.…”

A Member of the PLEIOTROPIC DRUG RESISTANCE Family of ATP Binding Cassette Transporters Is Required for the Formation of a Functional Cuticle in Arabidopsis

“…In plants, only a few in vivo substrates have been identified, such as cadmium and auxin for ABCG36 (PDR8), 2,4-dichlorophenoxy acetic acid for ABCG37 (PDR9), and abscisic acid for ABCG40 (PDR12; Kim et al, 2007;Strader and Bartel, 2009;Kang et al, 2010). However, there is some evidence that plant PDRs may transport a variety of secondary metabolites, including antimicrobial and antifungal compounds, as well as xenobiotics and heavy metals (Martinoia et al, 2002;Campbell et al, 2003;Moons, 2003;Ducos et al, 2005;Stukkens et al, 2005;Ito and Gray, 2006;Kobae et al, 2006;Stein et al, 2006;Krattinger et al, 2009).…”

Identification of genes expressed during the compatible interaction of grapevine with Plasmopara viticola through suppression subtractive hybridization (SSH)

“…There is also increasing evidence for the involvement of ABC transporters in defence. Stukkens et al (2005) found that a PDR protein in tobacco leaves (NpPDR1) was induced following pathogen attack and silencing of NpPDR1 by RNA interference resulted in increased sensitivity to the plant pathogen Botrytis cinerea. More recently Kobae et al (2006) demonstrated that a loss of an Arabidopsis plasma membrane-localized PDR ABC transporter (AtPDR8) lead to hypersensitive cell death upon pathogen infection.…”