Four Xenarthra species appear on the list of mammals whose presence has been documented in the Iguaçu National Park (INP). We conducted the first long-term camera trap monitoring in the Iguaçu region, encompassing the park and its buffer zone. We confirmed the presence of the four Xenarthra species known to occur in the park, with the first photographic records of giant anteaters (Myrmecophaga tridactyla). We also detected the presence of the greater naked-tailed armadillo (Cabassous tatouay), which had not previously been reported for the park. Giant anteater sightings are described, and habits are discussed. Our data provide important additions to the existing knowledge on giant anteaters inhabiting INP, a key wildlife refuge in southern Brazil.