Novel ZnO-binding peptides obtained by the screening of a phage display peptide library

Abstract

Zinc oxide (ZnO) is a semiconductor compound with a potential for wide use in various applications, including biomaterials and biosensors, particularly as nanoparticles (the size range of ZnO nanoparticles is from 2 to 100 nm, with an average of about 35 nm). Here, we report isolation of novel ZnO-binding peptides, by screening of a phage display library. Interestingly, amino acid sequences of the ZnO-binding peptides reported in this paper and those described previously are significantly different. This suggests that there is a high variability in sequences of peptides which can bind particular inorganic molecules, indicating that different approaches may lead to discovery of different peptides of generally the same activity (e.g., binding of ZnO) but having various detailed properties, perhaps crucial under specific conditions of different applications.

Chitosan Nanoparticles as Carriers for the Delivery of ΦKAZ14 Bacteriophage for Oral Biological Control of Colibacillosis in Chickens

“…These are possible conditions required for the storage of this formulated loaded ΦKAZ14 bacteriophage product to remain viable. Consistent with this finding, Golec et al [23] have demonstrated that tailed phages could be stored inside infected cells at −80 °C without a major loss of phage and host viability, which may seem a similar scenario to encapsulation of ΦKAZ14 bacteriophage in CS-NPs where it remained protected and maintained its viability under similar storage conditions. Similarly, Escherichia coli bacteriophage T4 (ATCC ® 11303-B41™, Manassas, VA, USA).…”

Bacteriophage T4 can produce progeny virions in extremely slowly growingEscherichia colihost: comparison of a mathematical model with the experimental data

“…Their antibacterial properties are used in phage therapy – against antibiotic‐resistant bacteria in humans (Takemura‐Uchiyama et al ., ) and animals (Kropinski et al ., ), to combat bacterial infections in fungi and plants (Adriaenssens et al ., ; Sajben‐Nagy et al ., ), and in food industry as antibacterial components of forage (García et al ., ). Furthermore, phages are used in phage display technique (Sidhu, ; Golec et al ., ) and as potential therapeutic gene‐delivery vehicles or as vehicles for the delivery of vaccines (Clark & March, ; Tao et al ., ). On the other hand, their antibacterial properties cause substantial financial losses in the biofermentation industry (Marcó et al ., ).…”

Inclusion of Zinc Oxide Nanoparticles into Virus-Like Peptide Nanocapsules Self-Assembled from Viral β-Annulus Peptide

“…Herein, we designed a novel β-annulus peptide connected via a ZnO-binding sequence at the N -terminal, which is directed toward the interior of the peptide nanocapsules. To date, several ZnO-binding peptides have been developed by the screening of peptide libraries [ 50 , 51 , 52 , 53 ]. We employed the HCVAHR sequence, developed by Okochi et al [ 54 ], as the ZnO-binding peptide, because this short peptide exhibits the highest affinity toward ZnO among the reported ZnO-binding peptides [ 55 ].…”