Bacteriophage T4 can produce progeny virions in extremely slowly growing<i>Escherichia coli</i>host: comparison of a mathematical model with the experimental data

Golec, Piotr; Karczewska-Golec, Joanna; Łoś, Marcin; Węgrzyn, Grzegorz

doi:10.1111/1574-6968.12372

Cited by 46 publications

(61 citation statements)

References 35 publications

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“…Form of selected equations used for fitting was chosen to reflect some relevant underlying biologic or physical mechanism. When the dilution rate increased, the latent period decreased and the burst size increased (Figure b & c), so both parameters changed in the similar fashion as it has been already described in the literature (Abedon et al., ; Golec et al., ; Hadas et al., ). It is interesting, that burst size linearly ( R 2 = .9753) increased with increasing dilution rate in the studied range (Figure c).…”

Section: Discussionsupporting

confidence: 75%

“…Latent period and burst size were determined by performing one‐step growth protocol in three chemostat experiments for each dilution rate (Golec et al., ; Hyman & Abedon, ). One milliliter of stabilized E. coli culture was transferred into a 1.5 ml Eppendorf tube.…”

Section: Methodsmentioning

confidence: 99%

“…In their experimental design, different media compositions were used to control the bacterial growth rate. On the other hand, Golec and colleagues used the same phage and bacterial strain as in our case (phage T4 and E. coli K‐12 MG1655, respectively), and different bacterial growth rates were achieved by varying the dilution rate in chemostat (Golec et al., ). It was revealed that latent period and burst size of phage T4 depend on bacterial growth rate.…”

Section: Introductionmentioning

confidence: 99%

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Effect of bacterial growth rate on bacteriophage population growth rate

2017

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It is important to understand how physiological state of the host influence propagation of bacteriophages (phages), due to the potential higher phage production needs in the future. In our study, we tried to elucidate the effect of bacterial growth rate on adsorption constant (δ), latent period (L), burst size (b), and bacteriophage population growth rate (λ). As a model system, a well‐studied phage T4 and Escherichia coli K‐12 as a host was used. Bacteria were grown in a continuous culture operating at dilution rates in the range between 0.06 and 0.98 hr−1. It was found that the burst size increases linearly from 8 PFU·cell−1 to 89 PFU·cell−1 with increase in bacteria growth rate. On the other hand, adsorption constant and latent period were both decreasing from 2.6∙10‐9 ml·min−1 and 80 min to reach limiting values of 0.5 × 10‐9 ml·min−1 and 27 min at higher growth rates, respectively. Both trends were mathematically described with Michaelis–Menten based type of equation and reasons for such form are discussed. By applying selected equations, a mathematical equation for prediction of bacteriophage population growth rate as a function of dilution rate was derived, reaching values around 8 hr−1 at highest dilution rate. Interestingly, almost identical description can be obtained using much simpler Monod type equation and possible reasons for this finding are discussed.

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Section: Discussionsupporting

confidence: 75%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of bacterial growth rate on bacteriophage population growth rate

2017

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“…Studies have shown that bacteriophage lytic development can be inhibited proficiently by carbon source limitation in bacterial cultures [27]. Development of the phages depend on intracellular resources of their hosts, which in turn depend on the physiological state of their hosts [28][29][30]. ɸSEP2 and ɸSEP3 survived at all conditions studied with a maximum of one log reduction when the host was in starved state ( Figure 5A & 5B).…”

Section: Survival Under Nutrient-deprived Conditionsmentioning

confidence: 96%

Infectivity Criteria for use of ɸSEP2 and ɸSEP3 as Bio-control Agents

Bhat

2017

JBMOA

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The emergence of multi-drug resistant Salmonella, one of the major pathogens causing food poisoning is challenging. Salmonella Enteritidis infections in poultry are widespread and the quest for safe alternatives to antibiotics led to phages. The purpose of this study was to isolate and characterize a novel pathogenic/lytic phages that targets Salmonella and to investigate its efficacy at lysing the bacterium. Poultry isolated Salmonella BT55CW showed considerable antibiotic resistance with MAR index of 0.4. Two lytic phages ɸSEP2 and ɸSEP3 were isolated using BT55CW Salmonella strain to control Salmonella infections. Morphology, host range and growth kinetics were studied. Influence of various physical and chemical factors such as pH, temperature, NaCl concentration and survival under different nutrient deprived conditions on the infectivity and persistence of phage forms the crux of the study. By TEM analysis ɸSEP2 and ɸSEP3 showed typical features of Podoviridae and Myoviridae respectively. Both lysed 60% all the Salmonella strains tested, survived at nutrition depleted conditions and substantially reduced Salmonella growth. Both phages showed considerable stability in the range of pH and temperature studied, but ɸSEP3 was less susceptible and hence a better candidate of the two as bio-control agent.Keywords: Salmonella-Phage-Bio control-Gastroenteritis-antibiotic-resistance; ɸSEP3; BT55CW Salmonella strain Citation: Sritha KS, Shemymol KA, Bhat SG (2017) Infectivity Criteria for use of ɸSEP2 and ɸSEP3 as Bio-control Agents. J Bacteriol Mycol Open Access 5(4): 00140.

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“…The latest reports describe phage multiplication under exceedingly slow host's growth rates (Golec et al, 2014) and stochasticity of adsorption rates (Galet et al, 2012) or lysis time (Dennehy and Wang, 2011) of certain mutants, but to the best of our knowledge none has been concerned with the question posed here: what growth conditions would bring about highest titers in wild-type strains.…”

Section: Introductionmentioning

confidence: 99%