2017
DOI: 10.1371/journal.pone.0187429
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Novel viruses in salivary glands of mosquitoes from sylvatic Cerrado, Midwestern Brazil

Abstract: Viruses may represent the most diverse microorganisms on Earth. Novel viruses and variants continue to emerge. Mosquitoes are the most dangerous animals to humankind. This study aimed at identifying viral RNA diversity in salivary glands of mosquitoes captured in a sylvatic area of Cerrado at the Chapada dos Guimarães National Park, Mato Grosso, Brazil. In total, 66 Culicinae mosquitoes belonging to 16 species comprised 9 pools, subjected to viral RNA extraction, double-strand cDNA synthesis, random amplificat… Show more

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Cited by 37 publications
(35 citation statements)
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References 51 publications
(66 reference statements)
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“…Scaphoideus titanus-associated partiti-like virus 1 clustered with two viruses identified in nematodes and formed a distinct clade only distantly related to Deltapartitivirus, the phylogenetically closest genus among those officially recognized by the ICTV. This result was consistent with phylogenetic analyses presented in previous works [41,42], especially in the manuscript by Shi et al [28], which remarked that in the Partitiviridae family it is possible to resolve well-supported clades that appear to be specific to invertebrate hosts. Despite that, conclusive evidence that a partiti-like virus could infect insects has never been reported in literature, so we preferred the indication "associated virus".…”
Section: Discussionsupporting
confidence: 92%
“…Scaphoideus titanus-associated partiti-like virus 1 clustered with two viruses identified in nematodes and formed a distinct clade only distantly related to Deltapartitivirus, the phylogenetically closest genus among those officially recognized by the ICTV. This result was consistent with phylogenetic analyses presented in previous works [41,42], especially in the manuscript by Shi et al [28], which remarked that in the Partitiviridae family it is possible to resolve well-supported clades that appear to be specific to invertebrate hosts. Despite that, conclusive evidence that a partiti-like virus could infect insects has never been reported in literature, so we preferred the indication "associated virus".…”
Section: Discussionsupporting
confidence: 92%
“…Four main findings can be reported regarding EVE clades: (i) the presence of various high-identical EVE copies, indicated by clades containing several sequences with near-zero branch lengths ( Figures 5B,D); (ii) the presence of glycoproteins inside BEL-Pao retroelements from RepBase intervening into various EVE clades ( Figure 5C); (iii) the presence of several sologlycoproteins embedded into retroelements clades ( Figure 5B), and (iv) the presence of sequences described as bona fide chuviruses (Lara Pinto et al, 2017;Pauvolid-Corrêa et al, 2016) inside and outside of clades mostly composed of EVE copies ( Figure 5D).…”
Section: Evidence Of a Chuvirus Env-like Protein Captured By Elementsmentioning
confidence: 99%
“…Insects were separated based on the ovitrap location and species. The number of mosquitos in each pool processed was based on previous literature reports [27–30] as well as the efficiency of RNA extraction kit; as a result, we used mosquito pools raging from 3 to 33 insects. The processing of mosquito pools requires a sensitive molecular method, since the minimum infection rate in females mosquitoes ranges from 0.1 to 3.9 per 1000 [31].…”
Section: Resultsmentioning
confidence: 99%