Novel Uses for Hollow Fiber Bioreactors

Hirschel, Mark; Gangemi, David J.; McSharry, James J.; Myers, Chris

doi:10.1089/gen.31.12.16

Cited by 9 publications

(9 citation statements)

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“…Equations 1, (2), and (3) yield Table 3 This deconvolution process was applied to all PolyhHbs synthesized in this study. The results are shown in Figure 5.…”

Section: Spectral Deconvolutionmentioning

confidence: 99%

“…To date, there is no practical O 2 carrier for use in HF bioreactors such as those employed as a bioartificial liver assist devices. 2 Because red blood cells (RBCs) undergo hemolysis in these devices, blood is an ineffective choice for use as an O 2 carrier in the perfusate. 3 Therefore, the goal of this work is to create a perfusate that can adequately oxygenate the high cell density found in the extracapillary space (ECS) of HF bioreactors.…”

Section: Introductionmentioning

confidence: 99%

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Next‐generation polymerized human hemoglobins in hepatic bioreactor simulations

Cuddington

Moses

Belcher

et al. 2020

Biotechnology Progress

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Hepatic hollow fiber (HF) bioreactors can be used to provide temporary support to patients experiencing liver failure. Before being connected to the patient's circulation, cells in the bioreactor must be exposed to a range of physiological O 2 concentrations as observed in the liver sinusoid to ensure proper performance. This zonation in cellular oxygenation promotes differences in hepatocyte phenotype and may better approximate the performance of a real liver within the bioreactor. Polymerized human hemoglobin (PolyhHb) locked in the tense quaternary state (T-state) has the potential to both supply and regulate O 2 transport to cultured hepatocytes in the bioreactor due to its low O 2 affinity. In this study, T-state PolyhHb production and purification processes were optimized to minimize the concentration of low-molecularweight PolyhHb species in solution. Deconvolution of size-exclusion chromatography spectra was performed to calculate the distribution of polymeric Hb species in the final product. Fluid flow and mass transport within a single fiber of a hepatic HF bioreactor was computationally modeled with finite element methods to simulate the effects of employing T-state PolyhHb to facilitate O 2 transport in a hepatic bioreactor system. Optimal bioreactor performance was defined as having a combined hypoxic and hyperoxic volume fraction in the extracapillary space of less than 0.05 where multiple zones were observed. The Damköhler number and Sherwood number had strong inverse relationships at each cell density and fiber thickness combination. These results suggest that targeting a specific Damköhler number may be beneficial for optimal hepatic HF bioreactor operation. K E Y W O R D S fluid flow modeling, hemoglobin-based oxygen carrier, hollow fiber bioreactor, polymerized human hemoglobin, red blood cell substitute

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“…Equations 1, (2), and (3) yield Table 3 This deconvolution process was applied to all PolyhHbs synthesized in this study. The results are shown in Figure 5.…”

Section: Spectral Deconvolutionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Next‐generation polymerized human hemoglobins in hepatic bioreactor simulations

Cuddington

Moses

Belcher

et al. 2020

Biotechnology Progress

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“…Furthermore, there are more adherently cultivated production cell lines than in antibody production processes, including the African green monkey kidney-derived Vero cells, Madin-Darby Canine Kidney (MDCK) cells or Chick Embryo Fibroblast (CEF) cells (Chaubard et al 2010;Hu et al 2008). Therefore, bioreactors are used which allow 2D cell growth, such as roller bottles and multitray systems, or which operate with hollow fibers (Hirschel 2011) or microcarriers (Moncaubeig 2013). Many vaccine producers (e.g.…”

Section: Viral Vaccine and Virus-like Particle Productionmentioning

confidence: 99%

Single-Use Bioreactors for Animal and Human Cells

et al. 2014

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Single-use (SU) bioreactors are being increasingly used in production processes based on animal (i.e. mammalian and insect) and human cells. They are particularly suitable for the production of high-value products on small and medium scales, and in cases where fast and safe production is a requirement. Thus, it is not surprising that SU bioreactors have established themselves for screening studies, cell expansions, and product expressions where they are used for the production of pre-clinical and clinical samples of therapeutic antibodies and preventive vaccines. Furthermore, recent publications have revealed the potential of SU bioreactors for the production of cell therapeutics using human mesenchymal stem cells (hMSCs).This chapter provides a perspective on current developments in SU bioreactors and their main applications. After briefly introducing the reader to the basics of SU bioreactor technology (terminology, historical milestones and characteristics compared to their reusable counterparts) an overview of the categories of currently available SU bioreactor types is provided. SU bioreactor instrumentation is then examined, before discussing well-established and novel applications of SU bioreactors for animal and human cells. This includes descriptions of the engineering characteristics of often-used types of SU bioreactors, covering wave-mixed, stirred, orbitally shaken systems and fixed-bed systems. In this context, the scaling-up of geometrically and non-geometrically similar SU bioreactors is also addressed.

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“…........................ Figure 20 Basic hollow fibre bioreactor design and the HF Primer™ small-scale bioreactor (Hirschel et al, 2011)…”

Section: Figure 19mentioning

confidence: 99%

“…Figure 20 Basic hollow fibre bioreactor design and the HF Primer™ small-scale bioreactor (Hirschel et al, 2011). Nguyen et al (2005) presented a flow-type hollow fibre bioreactor with the aim of improving the delivery of nutrients.…”

Section: Hollow Fibre Bioreactormentioning

confidence: 99%

Optimization of a perfusion bioreactor for tissue engineering

Freitas¹

2014

Biodental Engineering III

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Novel Uses for Hollow Fiber Bioreactors

Cited by 9 publications

References 0 publications

Next‐generation polymerized human hemoglobins in hepatic bioreactor simulations

Next‐generation polymerized human hemoglobins in hepatic bioreactor simulations

Single-Use Bioreactors for Animal and Human Cells

Optimization of a perfusion bioreactor for tissue engineering

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