Novel Use of Fluorescein Dye in Detection of Oral Dysplasia and Oral Cancer

Qaiser, Darakhshan; Sood, Anubhuti; Mishra, Deepika; Kharbanda, OP; Srivastava, Anurag; Gupta, Siddhartha Datta; Pandey, RM; Yadav, Rahul; Bhatt, Krushna; Kumawat, Ramniwas

doi:10.1016/j.pdpdt.2020.101824

Cited by 14 publications

(14 citation statements)

References 36 publications

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“…The results showed that Toluidine blue is a noninvasive method that offers real-time clinical information that may aid in completing a biopsy, biopsy site selection, and referral, in contrast to brush cytology, which requires specimen collection and a laboratory procedure. The absolute result was obtained by Fluorescein dye in the study conducted in 2020 by Qaiser et al [60], with 95% of sensitivity. Fluorescein has been tested for the very first time for oral diagnosis of OPMDs, while it has been previously used for cancer diagnosis in the stomach, colon, breast, and brain [60].…”

Section: Discussionmentioning

confidence: 94%

“…The absolute result was obtained by Fluorescein dye in the study conducted in 2020 by Qaiser et al [60], with 95% of sensitivity. Fluorescein has been tested for the very first time for oral diagnosis of OPMDs, while it has been previously used for cancer diagnosis in the stomach, colon, breast, and brain [60]. The study was conducted at a tertiary care dental center and included 100 individuals.…”

Section: Discussionmentioning

confidence: 94%

“…The dye is of organic origin, yellow, hypoallergenic, and nontoxic for topical use and produces an intense green-fluorescence in slightly acid to alkaline solutions. The study concluded that Fluorescein is an affordable and accessible technique, and it may find a promising role in community-based screening programs for oral cancer, especially in low-and middle-income regions, due to it being a safe and cost-effective measure [60,89].…”

Section: Discussionmentioning

confidence: 99%

“…Included studies (Table 1) were published between 2006 and 2020, and they were focused on the Autofluorescence technique (n:14) [28][29][30][31][32][33][34][35][36][37][38][39][40][41], HRME (n:5) [42][43][44][45][46], Optical spectroscopy (n:7) [10, [47][48][49][50][51][52], NBI (n:2) [53,54], and Vital Stain Colorants (n:6) [55][56][57][58][59][60]. In total, 34 studies were performed in adults (18 years of age or older).…”

Section: Study Characteristicsmentioning

confidence: 99%

“…According to the Newcastle-Ottawa scale (NOS) on cross-sectional (n:24) [28,29,31,35,37,39,40,[42][43][44][45][46][48][49][50][54][55][56]59,60,[73][74][75][76]; case-control (n:10) [10,32,47,[51][52][53][77][78][79][80] and cohort studies (n:7) [30,34,36,38,41,57,58] the authors evaluated the qualities of all included studies based on object selection, comparability, and exposure. A star was described as an appropriate entry, with each star representing one point.…”

Section: Total Score=mentioning

confidence: 99%

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In Vivo Imaging-Based Techniques for Early Diagnosis of Oral Potentially Malignant Disorders—Systematic Review and Meta-Analysis

Mazur

Ndokaj

Venugopal

et al. 2021

IJERPH

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Objectives: Oral potentially malignant disorders (OPMDs) are lesions that may undergo malignant transformation to oral cancer. The early diagnosis and surveillance of OPMDs reduce the morbidity and mortality of patients. Diagnostic techniques based on medical images analysis have been developed to diagnose clinical conditions. This systematic review and meta-analysis aimed to evaluate the efficacy of imaging-based techniques compared to the gold standard of histopathology to assess their ability to correctly identify the presence of OPMDs. Design: Literature searches of free text and MeSH terms were performed using MedLine (PubMed), Scopus, Google Scholar, and the Cochrane Library (from 2000 to 30 June 2020). The keywords used in the search strategy were: (“oral screening devices” or “autofluorescence” or “chemiluminescence” or “optical imaging” or “imaging technique”) and (“oral dysplasia” or “oral malignant lesions” or “oral precancerosis”). Results: The search strategy identified 1282 potential articles. After analyzing the results and applying the eligibility criteria, the remaining 43 papers were included in the qualitative synthesis, and 34 of these were included in the meta-analysis. Conclusions: None of the analyzed techniques based on assessing oral images can replace the biopsy. Further studies are needed to explore the role of techniques-based imaging analysis to identify an early noninvasive screening method.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

Section: Total Score=mentioning

confidence: 99%

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In Vivo Imaging-Based Techniques for Early Diagnosis of Oral Potentially Malignant Disorders—Systematic Review and Meta-Analysis

Mazur

Ndokaj

Venugopal

et al. 2021

IJERPH

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Development of multimodal micro‐endoscopic system with oblique illumination for simultaneous fluorescence imaging and spectroscopy of oral cancer

Thapa

Singh

Bhatt

et al. 2022

Journal of Biophotonics

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Multimodality of an optical system implies the use of one or more optical techniques to improve the system's overall performance and maximum utility. In this article, we demonstrate a multimodal system with oblique illumination that combines two different techniques; fluorescence micro‐endoscopy and spectroscopy simultaneously and can be utilized to obtain diverse information from the same location of biological sample. In present system, use of graded index (GRIN) rod‐lens makes it highly compact and oblique incidence decouples illumination geometry with collection geometry, preventing CCD cameras from saturation and reduces number of optical elements, thereby making system further miniaturized and field‐portable. It also overcomes the disadvantages of undesired reflections from different optical elements. The experimental results of simultaneous imaging and spectroscopy of the biological samples are presented along with quantitative spectroscopic parameters; peak wavelength shift, area under the curve and full width half maximum (FWHM). The spatial resolution, spectral resolution and field of view of the system are found to be 4.38 μm, 0.5 nm and 2.071×1.5480.25emmm2, respectively. Furthermore, we have obtained the red shift for cancerous oral tissue with respect to normal oral tissue 5.79 ± 1.071 nm. This could be important indicator for oral cancer screening.

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A Scoping Review of Ongoing Fluorescence‐Guided Surgery Clinical Trials in Otolaryngology

et al. 2021

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Objective: Fluorescence-guided surgery (FGS) is a rapidly developing intraoperative technology, and many contrast agents are currently under investigation. We sought to provide a review of the current state of FGS clinical trials in Otolaryngology, emphasizing its oncologic applications.Methods: According to the preferred reporting Items for systematic reviews and meta-analyses (PRISMA) workflow for scoping reviews, a clinical trial search was performed across multiple international clinical trials registries, searching for permutations of "fluorescence," "tumor," "surgery," and "nerve" to identify all relevant studies. Studies that were active, enrolling, or soon to be enrolling patients undergoing head and neck surgery were included.Results: Nineteen studies were eligible for inclusion. Seventeen studies are focused on FGS for oncologic resection and lymph node detection. One study assesses peripheral nerve fluorescence, and one evaluates normal parathyroid function after thyroidectomy. Contrast agents under development are conjugated to fluorophores that excite in the 800 nm (indocyanine green), 410 nm (5-aminolevulinic acid), 700 nm (Cyanine 5.5), and 525 nm ranges (fluorescein derivatives).Conclusion: Presently, there are 19 ongoing trials investigating novel FGS contrast agents for their safety, efficacy, and utility in Otolaryngology-Head and Neck Surgery. These agents rely on unique fluorophores and absorption ranges in the near-infrared and visible light spectra. FGS studies are expanding within Otolaryngology-Head and Neck Surgery with profound implications in oncologic surgery, lymph node detection, and anatomic and functional assessment.

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Novel Use of Fluorescein Dye in Detection of Oral Dysplasia and Oral Cancer

Cited by 14 publications

References 36 publications

In Vivo Imaging-Based Techniques for Early Diagnosis of Oral Potentially Malignant Disorders—Systematic Review and Meta-Analysis

In Vivo Imaging-Based Techniques for Early Diagnosis of Oral Potentially Malignant Disorders—Systematic Review and Meta-Analysis

Development of multimodal micro‐endoscopic system with oblique illumination for simultaneous fluorescence imaging and spectroscopy of oral cancer

A Scoping Review of Ongoing Fluorescence‐Guided Surgery Clinical Trials in Otolaryngology

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