Development of multimodal micro‐endoscopic system with oblique illumination for simultaneous fluorescence imaging and spectroscopy of oral cancer

Thapa, Pramila; Singh, Veena; Bhatt, Sunil; Tayal, Shilpa; Mann, Priyanka; Maurya, Kiran; Mishra, Deepika; Mehta, Dalip Singh

doi:10.1002/jbio.202100284

Cited by 7 publications

(24 citation statements)

References 35 publications

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“…Additionally, multimodal micro-endoscope is very easy to use and can be made hand-held for low-resource settings. Development of the multimodal micro-endoscope is reported earlier in [9]. In the present manuscript, a detailed study of OSCC and normal tissue from the micro-endoscope is reported.…”

Section: Discussionmentioning

confidence: 82%

“…Skewness, kurtosis and energy of the FL images gives the information about asymmetry, flatness and uniformity about image histogram and having a significant change in OSCC compared to normal. Peak wavelength of OSCC is red-shifted from the normal tissue which indicates the longer optical path length of the incident photon within the OSCC resulting increase reabsorption rate and thus red-shift [9]. In addition, max counts and AUC higher for OSCC.…”

Section: Methodsmentioning

confidence: 99%

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Low-cost multimodal micro-endoscopic system for oral cancer screening in low resource setting

Thapa

Bhatt²,

Mann³

et al. 2023

Optics and Biophotonics in Low-Resource Settings IX

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Oral squamous cell carcinoma (OSCC) is the most fatal cancer and has poor survival rate among all cancers. Regular screening of patients can reduce fatality rate of OSCC. Usually, oral cancer screening is accomplished by visual examinations and conventional endoscopes. These regular conventional endoscopes are not able to provide microstructure of altered OSCC tissue so use of micro-endoscopes are favorable for cancer screening, since it can give morphology of altered OSCC tissue. Similarly, the fluorescence (FL) spectroscopy of OSCC tissue can probe the molecular levels of tissue giving information about the progression of oral cancer as it is biophysical and biochemical sensitive. The motivation behind this study is to analyze OSCC by simultaneous micro-endoscopy and spectroscopy with the help of in-house-fabricated low-cost micro-endoscopic system. Simultaneous FL imaging with spectroscopy can provide the morphological changes in altered tissue and record the corresponding intensity counts, wavelength shift and area under the curve (AUC) of the sample. These spectroscopic parameters are directly linked with molecular transitions within the tissue and can be used in real-time cancer screening. FL micro-endoscopic images gives information about fluorescence contrast distribution within the tissue and differ in mean intensity, skewness, kurtosis and energy for OSCC and normal tissue. Present study included 10 OSCC patients and 8 control patients. The value of mean intensity, skewness, kurtosis, energy, peak wavelength, AUC and max counts for OSCC is 163.3±30, -0.567±0.33, 7.82±5.2, ~4.24×105 , 533.28±1.73, ~4.5×106 , ~4.05×104 , respectively and for normal tissue is 63.5±22.4, 0.148±0.05, 4.89±3.89, ~1.65×105 , 528.89±0.81, ~3.3×106 , ~4.63×103 , respectively. The observations from the present study are in-line with screening requirements of OSCC in regular basis screening and suitable for low-resource setting area.

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Section: Discussionmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Low-cost multimodal micro-endoscopic system for oral cancer screening in low resource setting

Thapa

Bhatt²,

Mann³

et al. 2023

Optics and Biophotonics in Low-Resource Settings IX

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“…All mentioned conventional techniques are time-consuming, invasive, and need expertise [6][7][8]. The increasing rate of breast cancer leads to different evaluation methods including optical techniques like fluorescence spectroscopy [9][10][11][12]. Fluorescence spectroscopy targets the molecular levels of human tissue probing different chemical compositions, complex conjugates, metabolites, etc., and is able to use in earlystage diagnosis as well.…”

Section: Introductionmentioning

confidence: 99%

“…Fluorescence spectroscopy targets the molecular levels of human tissue probing different chemical compositions, complex conjugates, metabolites, etc., and is able to use in earlystage diagnosis as well. Optical techniques offer non-invasiveness, nonionizing radiation, and visualization of soft tissues depending on optical properties [12]. The changes in the concentrations and microenvironments of fluorophores alter tissue fluorescence to a sufficient extent to detect metabolic and pathological changes related to precancerous and cancerous growth, even though fluorescence measurements are not capable of detailing structural changes in tissues [12].…”

Section: Introductionmentioning

confidence: 99%

Analysis of early-stage breast cancer using smartphone-based fluorescence imaging and spectroscopy during intra-operative procedure

Thapa

Singh²,

Gupta³

et al. 2023

Optical Biopsy XXI: Toward Real-Time Spectroscopic Imaging and Diagnosis

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Breast cancer is most common cancer among women world-wide and can be treated if diagnosed at early-stage. Fluorescence (FL) techniques have a considerable impact in tumor detection as FL is highly sensitive to biochemical and biophysical structure of the tissues, providing novel techniques for early and noninvasive diagnosis of cancer. Due to high sensitivity of FL, it can be used in early-stage breast cancer detection. Fluorescence-guided-surgery (FGS) using exogenous agent in breast cancer is a well-known method, which locates tumor and margins during intra-operative procedure. FL-spectroscopy and FL-imaging has shown the potentiality in FGS of breast cancer independently. Combination of these two modalities in a single system improves the success rate of FGS which can give tumor free tissues. The goal of the current study is to analyze and classify the early-stage breast cancer, locally advanced breast cancer (invasive ductal carcinoma) and normal tissue. For that, we combine FL-imaging and FL-spectroscopy in a single smartphone-based point-of-care devices and recorded data during intra-operative procedure. A total 21 patients of invasive ductal carcinoma and fibroadenoma are included in this study. Total 65 FL-spectra are recorded during intraoperative procedure which are further used in support vector machine (SVM) based classification of fibroadenoma, invasive ductal carcinoma (IDC) and normal tissue. The sensitivity, specificity, positive predictive value, negative predictive value, and overall diagnostic accuracy (total efficiency) is 78.6 %, 90%, 91.6%, 75%, 95%, respectively. Additionally, we observed a red shift in case of IDC and fibroadenoma from normal tissue, which is 5.22±1.77 nm, and 4.96±2.61 nm, respectively.

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“…Moreover, FSS is a pH dependent dye and tags with more alkaline nature. Intracellular pH value of the cancer cells is more alkaline, therefore, FSS tags with cancer cells in higher amounts giving a significant fluorescence contrast to cancerous tissue 30 …”

mentioning

confidence: 99%

Point‐of‐care devices based on fluorescence imaging and spectroscopy for tumor margin detection during breast cancer surgery: Towards breast conservation treatment

et al. 2023

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Objective Fluorescence‐based methods are highly specific and sensitive and have potential in breast cancer detection. Simultaneous fluorescence imaging and spectroscopy during intraoperative procedures of breast cancer have great advantages in detection of tumor margin as well as in classification of tumor to healthy tissues. Intra‐operative real‐time confirmation of breast cancer tumor margin is the aim of surgeons, and therefore, there is an urgent need for such techniques and devices which fulfill the surgeon's priorities. Methods In this article, we propose the development of fluorescence‐based smartphone imaging and spectroscopic point‐of‐care multi‐modal devices for detection of invasive ductal carcinoma in tumor margin during removal of tumor. These multimodal devices are portable, cost‐effective, noninvasive, and user‐friendly. Molecular level sensitivity of fluorescence process shows different behavior in normal, cancerous and marginal tissues. We observed significant spectral changes, such as, red‐shift, full‐width half maximum (FWHM), and increased intensity as we go towards tumor center from normal tissue. High contrast in fluorescence images and spectra are also recorded for cancer tissues compared to healthy tissues. Preliminary results for the initial trial of the devices are reported in this article. Results A total 44 spectra from 11 patients of invasive ductal carcinoma (11 spectra for invasive ductal carcinoma and rest are normal and negative margins) are used. Principle component analysis is used for the classification of invasive ductal carcinoma with an accuracy of 93%, specificity of 75% and sensitivity of 92.8%. We obtained an average 6.17 ± 1.66 nm red shift for IDC with respect to normal tissue. The red shift and maximum fluorescence intensity indicates p < 0.01. These results described here are supported by histopathological examination of the same sample. Conclusion In the present manuscript, simultaneous fluorescence‐based imaging and spectroscopy is accomplished for the classification of IDC tissues and breast cancer margin detection.

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Development of multimodal micro‐endoscopic system with oblique illumination for simultaneous fluorescence imaging and spectroscopy of oral cancer

Cited by 7 publications

References 35 publications

Low-cost multimodal micro-endoscopic system for oral cancer screening in low resource setting

Low-cost multimodal micro-endoscopic system for oral cancer screening in low resource setting

Analysis of early-stage breast cancer using smartphone-based fluorescence imaging and spectroscopy during intra-operative procedure

Point‐of‐care devices based on fluorescence imaging and spectroscopy for tumor margin detection during breast cancer surgery: Towards breast conservation treatment

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