Low-cost multimodal micro-endoscopic system for oral cancer screening in low resource setting

Abstract

Oral squamous cell carcinoma (OSCC) is the most fatal cancer and has poor survival rate among all cancers. Regular screening of patients can reduce fatality rate of OSCC. Usually, oral cancer screening is accomplished by visual examinations and conventional endoscopes. These regular conventional endoscopes are not able to provide microstructure of altered OSCC tissue so use of micro-endoscopes are favorable for cancer screening, since it can give morphology of altered OSCC tissue. Similarly, the fluorescence (FL) spectroscopy of OSCC tissue can probe the molecular levels of tissue giving information about the progression of oral cancer as it is biophysical and biochemical sensitive. The motivation behind this study is to analyze OSCC by simultaneous micro-endoscopy and spectroscopy with the help of in-house-fabricated low-cost micro-endoscopic system. Simultaneous FL imaging with spectroscopy can provide the morphological changes in altered tissue and record the corresponding intensity counts, wavelength shift and area under the curve (AUC) of the sample. These spectroscopic parameters are directly linked with molecular transitions within the tissue and can be used in real-time cancer screening. FL micro-endoscopic images gives information about fluorescence contrast distribution within the tissue and differ in mean intensity, skewness, kurtosis and energy for OSCC and normal tissue. Present study included 10 OSCC patients and 8 control patients. The value of mean intensity, skewness, kurtosis, energy, peak wavelength, AUC and max counts for OSCC is 163.3±30, -0.567±0.33, 7.82±5.2, ~4.24×105 , 533.28±1.73, ~4.5×106 , ~4.05×104 , respectively and for normal tissue is 63.5±22.4, 0.148±0.05, 4.89±3.89, ~1.65×105 , 528.89±0.81, ~3.3×106 , ~4.63×103 , respectively. The observations from the present study are in-line with screening requirements of OSCC in regular basis screening and suitable for low-resource setting area.

Design, fabrication and testing of a smartphone-based bimodal device for oral precancer diagnosis

“…In last several years, an increasing trend has been observed among researchers to develop smartphone based standalone and field portable optical systems for various research applications. [13][14][15][16] In this study, we have designed, fabricated and validated a 3D printed smartphone-based bimodal device for oral precancer diagnosis. The proposed device is able to record fluorescence images (FI) and fluorescence spectra (FS) on a mobile phone camera by utilising a simple and cost-effective optical geometry.…”