Novel Treatment Strategies for Biofilm-Based Infections

Vuotto, Claudia; Donelli, Gianfranco

doi:10.1007/s40265-019-01184-z

Cited by 50 publications

(53 citation statements)

References 277 publications

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“…We are optimistic that understanding the role the ADI pathway plays in antibiotic tolerance will lead to novel antibacterial therapies. Furthermore, unlike most of the current therapies for treating biofilm infections that require dispersing or mechanically breaking up biofilms before eradicating bacteria (38)(39)(40), disrupting the ADI pathway has the potential to finally provide a mechanism for the direct targeting of bacteria while they are still within a biofilm.…”

Section: Discussionmentioning

confidence: 99%

The Arginine Deiminase Pathway Impacts Antibiotic Tolerance during Biofilm-Mediated Streptococcus pyogenes Infections

Freiberg

Breton

Harro

et al. 2020

mBio

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ABSTRACT Bacterial biofilms are responsible for a variety of serious human infections and are notoriously difficult to treat due to their recalcitrance to antibiotics. Further work is necessary to elicit a full understanding of the mechanism of this antibiotic tolerance. The arginine deiminase (ADI) pathway is responsible for bacterial pH maintenance and is highly expressed during biofilm growth in multiple bacterial species. Using the group A Streptococcus (GAS) as a model human pathogen, the ADI pathway was demonstrated to contribute to biofilm growth. The inability of antibiotics to reduce GAS populations when in a biofilm was demonstrated by in vitro studies and a novel animal model of nasopharyngeal infection. However, disruption of the ADI pathway returned GAS biofilms to planktonic levels of antibiotic sensitivity, suggesting the ADI pathway is influential in biofilm-related antibiotic treatment failure and provides a new strategic target for the treatment of biofilm infections in GAS and potentially numerous other bacterial species. IMPORTANCE Biofilm-mediated bacterial infections are a major threat to human health because of their recalcitrance to antibiotic treatment. Through the study of Streptococcus pyogenes, a significant human pathogen that is known to form antibiotic-tolerant biofilms, we demonstrated the role that a bacterial pathway known for responding to acid stress plays in biofilm growth and antibiotic tolerance. This not only provides some insight into antibiotic treatment failure in S. pyogenes infections but also, given the widespread nature of this pathway, provides a potentially broad target for antibiofilm therapies. This discovery has the potential to impact the treatment of many different types of recalcitrant biofilm infections.

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Section: Discussionmentioning

confidence: 99%

The Arginine Deiminase Pathway Impacts Antibiotic Tolerance during Biofilm-Mediated Streptococcus pyogenes Infections

Freiberg

Breton

Harro

et al. 2020

mBio

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“…No commercially available antibiotic has been demonstrated to be fully effective against bacteria grown as biofilm on urinary catheters. To overcome that, in the past few decades a great effort has been devoted to the development of safe, effective, and fast-acting anti-biofilm molecules and strategies that do not confer evolutionary pressure to microorganisms [14] as well as antifouling and antimicrobial coatings for medical devices, such as urinary catheters, for the purpose of improving their long-term and broad-spectrum efficacy, and biocompatibility [15].…”

Section: Introductionmentioning

confidence: 99%

Poloxamer 338 Affects Cell Adhesion and Biofilm Formation in Escherichia coli: Potential Applications in the Management of Catheter-Associated Urinary Tract Infections

et al. 2020

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Poloxamers are nontoxic, amphiphilic copolymers used in different formulations. Due to its surfactant properties, Poloxamer 338 (P388) is herein proposed as a strategy to avoid biofilm formation often causing recalcitrant catheter-associated urinary tract infections (CAUTI). The aim is to evaluate the ability of P388 coatings to affect the adhesion of Ec5FSL and Ec9FSL Escherichia coli strains on silicone urinary catheters. Attenuated total reflection infrared spectroscopy, atomic force microscopy, and static water contact angle measurement were employed to characterize the P388-coated silicone catheter in terms of amount of P388 layered, coating thickness, homogeneity, and hydrophilicity. In static conditions, the antifouling power of P388 was defined by comparing the E. coli cells adherent on a hydrophilic P388-adsorbed catheter segment with those on an uncoated one. A P388-coated catheter, having a homogeneous coverage of 35 nm in thickness, reduced of 0.83 log10 and 0.51 log10 the biofilm of Ec5FSL and Ec9FSL, respectively. In dynamic conditions, the percentage of cell adhesion on P388-adsorbed silicone channels was investigated by a microfluidic system, simulating the in vivo conditions of catheterized patients. As a result, both E. coli isolates were undetected. The strong and stable antifouling property against E. coli biofilm lead us to consider P388 as a promising anti-biofilm agent for CAUTIs control.

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“…RSV inhibits the synthesis of the autoinducers N-acyl-homoserine lactones in Yersinia enterocolitica and Erwinia carotovora at a concentration that does not influence growth parameters [137,138]. RSV affects QS systems in E. coli [154] and Chromobacterium violaceum [139] via the uncharacterized method. Hence, RSV affects numerous virulence traits at concentrations up to 64 times lower than growth-inhibitory concentrations.…”

Section: Interference With Quorummentioning

confidence: 99%

A Comprehensive Study on the Antimicrobial Properties of Resveratrol as an Alternative Therapy

Abedini

Khodadadi

Zeinalzadeh

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Resveratrol is a polyphenolic antioxidant whose possible health benefits include anticarcinogenic, antiaging, and antimicrobial properties that have gained significant attention. The compound is well accepted by individuals and has been commonly used as a nutraceutical in recent decades. Its widespread usage makes it essential to study as a single agent as well as in combination with traditional prescription antibiotics as regards to antimicrobial properties. Resveratrol demonstrates the action of antimicrobials against a remarkable bacterial diversity, viruses, and fungus. This report explains resveratrol as an all-natural antimicrobial representative. It may modify the bacterial virulence qualities resulting in decreased toxic substance production, biofilm inhibition, motility reduction, and quorum sensing disturbance. Moreover, in conjunction with standard antibiotics, resveratrol improves aminoglycoside efficacy versus Staphylococcus aureus, while it antagonizes the deadly function of fluoroquinolones against S. aureus and also Escherichia coli. The present study aimed to thoroughly review and study the antimicrobial potency of resveratrol, expected to help researchers pave the way for solving antimicrobial resistance.

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Novel Treatment Strategies for Biofilm-Based Infections

Cited by 50 publications

References 277 publications

The Arginine Deiminase Pathway Impacts Antibiotic Tolerance during Biofilm-Mediated Streptococcus pyogenes Infections

The Arginine Deiminase Pathway Impacts Antibiotic Tolerance during Biofilm-Mediated Streptococcus pyogenes Infections

Poloxamer 338 Affects Cell Adhesion and Biofilm Formation in Escherichia coli: Potential Applications in the Management of Catheter-Associated Urinary Tract Infections

A Comprehensive Study on the Antimicrobial Properties of Resveratrol as an Alternative Therapy

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