Novel synovial fluid recovery method allows for quantification of a marker of arthritis in mice

Seifer, D.R.; Furman, Bridgette D.; Guilak, Farshid; Olson, Steven A.; Brooks, Sydney; Kraus, Virginia B.

doi:10.1016/j.joca.2008.04.013

Cited by 66 publications

(73 citation statements)

References 33 publications

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“…In small animal like mice, rats or guinea pigs, Whatman paper can be used to absorb synovial fluid. 39 Recently, Seifer et al 39 described a method for synovial fluid recovery using Melgisorb (Tendra, Sweden), a calcium sodium alginate compound (CSAC), used as a dressing on wounds to absorb heavy exudate. In dogs, joint lavage with saline has been used to collect synovial fluid and the dilution effect of the lavage has been corrected by normalization to serum urea.…”

Section: Discussionmentioning

confidence: 99%

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Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

et al. 2016

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Objective. Osteoarthritis (OA) is one of the leading causes of disability within the adult population. Currently, its diagnosis is mainly based on clinical examination and standard radiography. To date, there is no way to detect the disease at a molecular level, before the appearance of structural changes and symptoms. So an attractive alternative for monitoring OA is the measurement of biochemical markers in blood, urine, or synovial fluid, which could reflect metabolic changes in joint tissue and therefore disease onset and progression. Animal models are relevant to investigate the early stage of OA and metabolic changes occurring in joint tissues. The goal of this narrative review is to summarize the scientific data available in the literature on soluble biomarkers in animal models of OA. Design. A literature search was conducted using the PubMed/ Medline and Scopus databases between February 1995 and December 2015. All original articles, systematic and narrative reviews published in French or in English were considered. Results. We summarized the data of 69 studies and proposed a classification scheme for OA biomarkers in animal studies, largely inspired by the BIPEDS classification. Conclusions. Studies about biomarkers and animal models indicate that some markers could be valuable to monitor OA progression and assess therapeutic response in some animal models.

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Section: Discussionmentioning

confidence: 99%

“…8 COMP, MMP-3, and MMP-9 synovial fluid levels also increased in mice after acute joint injury induced by intraarticular fracture. 38,39 In a study of Porta et al, 40 the endocannabinoids anandamide (AEA) and 2-arachidonoylglycerol (2-AG) did not modify serum levels of MIA and control groups.…”

Section: Micementioning

confidence: 96%

Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

et al. 2016

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“…The differences in PTOA development between C57BL/6 mice and MRL/MpJ mice after IAF have been further explored [69]. Seifer et al utilized this mouse model to investigate cartilage oligomeric matrix protein (COMP) in synovial fluid of mice following IAF [70]. Lewis et al used this model to determine the effect of articular fracture severity on synovial inflammation, bone morphology, liberated fracture area, cartilage pathology, chondrocyte viability, and systemic cytokines and biomarkers levels [68].…”

Section: Non-invasive Mouse Models Of Ptoamentioning

confidence: 99%

Non-invasive mouse models of post-traumatic osteoarthritis

Christiansen

Guilak

Lockwood

et al. 2015

Osteoarthritis and Cartilage

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111

123

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Animal models of osteoarthritis (OA) are essential tools for investigating the development of the disease on a more rapid timeline than human OA. Mice are particularly useful due to the plethora of genetically modified or inbred mouse strains available. The majority of available mouse models of OA use a joint injury or other acute insult to initiate joint degeneration, representing post-traumatic osteoarthritis (PTOA). However, no consensus exists on which injury methods are most translatable to human OA. Currently, surgical injury methods are most commonly used for studies of OA in mice; however, these methods may have confounding effects due to the surgical/invasive injury procedure itself, rather than the targeted joint injury. Non-invasive injury methods avoid this complication by mechanically inducing a joint injury externally, without breaking the skin or disrupting the joint. In this regard, non-invasive injury models may be crucial for investigating early adaptive processes initiated at the time of injury, and may be more representative of human OA in which injury is induced mechanically. A small number of non-invasive mouse models of PTOA have been described within the last few years, including intra-articular fracture of tibial subchondral bone, cyclic tibial compression loading of articular cartilage, and anterior cruciate ligament rupture via tibial compression overload. This review describes the methods used to induce joint injury in each of these non-invasive models, and presents the findings of studies utilizing these models. Altogether, these non-invasive mouse models represent a unique and important spectrum of animal models for studying different aspects of PTOA.

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“…When joint lavage is used, biomarker levels should be corrected for dilution using serum and lavage fluid urea levels (37). Another published method for obtaining small quantities of synovial fluid is the use of paper or alginate to collect synovial fluid from a joint after harvest followed by digestion of the alginate or paper to isolate the synovial fluid constituents (38). The limited quantity of synovial fluid available for analysis necessitates careful choice of biomarkers to be included in studies using small animal OA models.…”

Section: Molecular Biomarkersmentioning

confidence: 99%

Animal Models of Osteoarthritis: Challenges of Model Selection and Analysis

Teeple

Jay

Elsaid

et al. 2013

AAPS J

174

192

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ABSTRACT. Osteoarthritis (OA) is the most common musculoskeletal disease, affecting millions of individuals worldwide. New treatment approaches require an understanding of the pathophysiology of OA and its biomechanical, inflammatory, genetic, and environmental risk factors. The purpose of animal models of OA is to reproduce the pattern and progression of degenerative damage in a controlled fashion, so that opportunities to monitor and modulate symptoms and disease progression can be identified and new therapies developed. This review discusses the features, strengths, and weaknesses of the common animal models of OA; considerations to be taken when choosing a method for experimental induction of joint degeneration; and the challenges of measuring of OA progression and symptoms in these models.

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Novel synovial fluid recovery method allows for quantification of a marker of arthritis in mice

Cited by 66 publications

References 33 publications

Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

Review of Soluble Biomarkers of Osteoarthritis: Lessons From Animal Models

Non-invasive mouse models of post-traumatic osteoarthritis

Animal Models of Osteoarthritis: Challenges of Model Selection and Analysis

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