Novel Spectrofluorimetric Method for the Determination of Perindopril Erbumine Based on Charge Transfer Reaction with 7-Hydroxycoumarin

Abstract

A novel, simple, selective and sensitive spectrofluorimetric method was developed and validated for the determination of perindopril erbumine using 7-hydroxycoumarin. Perindopril erbumine was found to react with 7-hydroxycoumarin in acetonitrile resulting in a new fluorescent product with about 58 nm blue shifted emission. The fluorescence of the complex was measured at 440 nm after excitation at 350 nm in acetonitrile. Under the optimum conditions, the fluorescence intensity was linear over a concentration range of 2.0-16.0 μg/mL (R(2) = 1) with a detection limit of 0.054 μg/mL. The proposed method was fully validated and successfully applied to the analysis of perindopril erbumine in pure form and tablets. Statistical comparison of the results obtained by the proposed and reference method revealed no significant differences in the performance of the two methods regarding the accuracy and precision respectively. The method was shown to be highly specific in the presence of indapamide, a diuretic that is commonly combined with perindopril erbumine. A proposal for the reaction pathway with 7-hydroxycoumarin was postulated.

Utilization of erythrosine B in spectrofluorimetric quenching analysis of amlodipine and perindopril in pharmaceutical and biological matrices

“…Perindopril (PER) is an antihypertensive nonsulfhydryl angiotensin‐converting enzyme (ACE) inhibitor that is used to treat hypertension and stable coronary artery disease with the nomenclature of (2 S ,3 aS ,7 aS )‐1‐[(2 S )‐2‐[[(2 S )‐1‐ethoxy‐1‐oxopentan‐2‐yl]amino]propanoyl]‐2,3,3 a ,4,5,6,7,7 a ‐octahydroindole‐2‐carboxylic acid (Figure 1b). For determining PER alone or in combination, various analytical procedures have been published including spectrophotometry [3, 4, 7, 8, 43–45], spectrofluorimetry [46–49], and chromatographic approaches including TLC [30], LC–MS/MS [31] and HPLC [4, 34–36, 39, 50, 51].…”

“…The approach was simple, sensitive, and validated according to International Council on Harmonization (ICH) guidelines. The proposed method had higher sensitivity than many of the previously reported methods [19, 20, 23, 25, 28, 46–49]. In addition, some of the reported methods required elevated temperature, were time consuming, and were carried out in the presence of concentrated acids [28, 49].…”

“…For determining PER alone or in combination, various analytical procedures have been published including spectrophotometry [3,4,7,8,[43][44][45], spectrofluorimetry [46][47][48][49], and chromatographic approaches including TLC [30], LC-MS/MS [31] and HPLC [4, 34-36, 39, 50, 51].…”

Determination of perindopril erbumine by oxidative coupling reaction using 2, 6-dichloroquinone-4-chlorimide

“…A survey of the literature revealed that UV (Nayak, Pillai, 2011;Kale et al, 2011), HPLC(El- Gizawy et al, 2014;Gizawy et al, 2013;Pattan, et al,2013;Singh et al, 2011), RP-HPLC (Chaudhary, Determination of perindopril erbumine by oxidative coupling reaction using 2, 6-dichloroquinone-4-chlorimide Patel, Chaudhary 2010;Mastannamma et al, 2016;Dugga, Peraman, Nayakanti, 2014;Joseph, Philip, 2011;Patel et al, 2011;Prajapati et al, 2011;Prameela Rani, Bala Sekaran, 2009), spectrofluorimetric (Fael Sakur 2015a. ;Fael Sakur 2015b), kinetic spectrophotometric (Rahman, Rahman, Haque, 2017;Rahman, Anwar, Kashif, 2006), LC-MS (Deepak, et al,2006) and GC-MS (Maurer, Kraemer, Arlt,1998) methods were reported for the estimation of PPE. There are limited spectrophotmetric methods (Sridevi,Jahnavi, Sekaran, 2012;Rahman,Rahman,Khatoon,2012;Rahman, Rahman 2011;Prameela Rani et al, 2012) are reported for the assay of perindopril erbumine (PPE).…”

Application of Oxidative Coupling Reaction using Brucine and Sodium Periodate as Chromogenic Reagent for the Assay of Perindopril Erbumine in Formulations

“…The drug (PDE) is listed in the British Pharmacopoeia (3), Remington (4), and Physician's desk reference (5). A survey of the literature revealed that UV (6,7), HPLC (8)(9)(10)(11), RP-HPLC (12)(13)(14)(15)(16)(17)(18), spectrofluorimetric (19,20), UV-Visible spectrophotometric (21)(22)(23)(24), kinetic spectrophotometric (25,26), LC-MS (27,28), and GC-MS (29) methods were reported for the estimation of PPE. It was found that there are very few spectrophotometric methods are reported for the assay of PPE.…”