Novel small molecular dye-loaded lipid nanoparticles with efficient near-infrared-II absorption for photoacoustic imaging and photothermal therapy of hepatocellular carcinoma

Chen, Qingshan; Chen, Jingqin; He, Mu; Bai, Yuanyuan; Yan, Huixiang; Zeng, Ning; Liu, Fangyan; Wen, Sai; Liu, Song; Sheng, Zonghai; Liu, Chengbo; Fang, Chihua

doi:10.1039/c9bm00528e

Cited by 48 publications

(36 citation statements)

References 58 publications

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“…The IR1061 dye a commercial dye with high NIR-II (1064 nm) absorbance and low fluorescence quantum yield, and holds promise as a NIR-II photothermal agent but the hydrophobicity of IR1061 limits its use in biomedical applications. [31][32][33] In order to overcome this limitation, further modifications of the IR1061 are required.…”

Section: Introductionmentioning

confidence: 99%

Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy

Li¹,

Qing²,

Li³

et al. 2020

IJN

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The aim of this study was to develop an "all-in-one" nanoplatform that integrates at the second near-infrared (NIR-II) region dye IR1061 and anticancer drug paclitaxel (PTX) into an apoferritin (AFN) nanocage (IR-AFN@PTX). Simultaneously, folic acid (FA), tumor target molecule, was conjugated onto IR-AFN@PTX to be IR-AFN@PTX-FA for tumortargeted and pH/NIR-II-triggered synergistic photothermal-chemotherapy. Methods: IR1061 was firstly reacted with PEG and then conjugated with AFN to be IR-AFN. Then, FA was conjugated onto the surface of IR-AFN to be IR-AFN-FA. At last, PTX was incorporated into IR-AFN-FA to fabricate a nanoplatform IR-AFN@PTX-FA. The NIR-II photothermal properties and pH/NIR-II triggered drug release were evaluated. The ability of IR-AFN@PTX-FA to target tumors was estimated using optical bioluminescence. In vitro and in vivo synergistic therapeutic effects of pH/NIR-II-triggered and tumor-targeted photothermal-chemotherapy were investigated in 4T1 tumor model. Results: IR-AFN@PTX-FA showed excellent water solubility and physiological stability, which significantly enhanced the solubility of both IR1061 and PTX. After 5 min of laser irradiation at 1064 nm, IR-AFN@PTX-FA exhibited an effective photothermal effect compared with laser irradiation at 808 nm, even when blocked with 0.6 cm thick chicken breast. Cellular uptake experiments showed IR-AFN@PTX-FA utilized clathrin-mediated and caveolae-mediated endocytosis pathways to enter 4T1 cells, and was then delivered by the endosome to the lysosome. NIR-II laser irradiation and pH could synergistically trigger PTX release, inducing significant tumor inhibition in vitro and in vivo. Conclusion: As a novel "all-in-one" nanoplatform, IR-AFN@PTX-FA was found to selectively target tumors and showed very efficient NIR-II photothermal effects and pH/NIR-II triggered drug release effects, showing a remarkable, synergistic photothermal-chemotherapy effect.

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Section: Introductionmentioning

confidence: 99%

Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy

Li¹,

Qing²,

Li³

et al. 2020

IJN

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“…To broaden the applications of PAI modality and to improve the sensitivity, specificity, and contrast of PAI further, several exogenous contrast agents with strong NIR absorption and high photothermal conversion efficiency were developed. We discussed the recently reported PA exogenous contrast agents, such as metallic nanomaterials (Au/Ag (T. Kim et al, ), AuPB (J. Zhou, Jiang, et al, )), QDs (MoO 3‐ x ; Ding et al, ), small molecule ICG dye (P. K. Upputuri & Pramanik, ), IR‐1061 dye‐loaded lipid NPs (Q. Chen, Chen, et al, ), biodegradable Biliverdin NPs (Fathi et al, ), organic dyed droplets (P. Zhang et al, ), semiconducting polymer nanoparticles (B. Guo et al, ; Jiang, Upputuri, et al, ; Miao & Pu, ; P. K. Upputuri et al, ; L. Zhou, Zhou, & Wu, ). We classified these PA contrast agents into inorganic and organic materials based on their chemical structure, and summarized their advantages and disadvantages in Table .…”

Section: Discussionmentioning

confidence: 99%

“…In parallel, other classes of dyes such as methylene blue, Evans blue, IRdye800, and so on (Laramie, Smith, Marmarchi, McNally, & Henary, ), have been explored as contrast agents for molecular PA imaging. Recently, a new type of small molecular dye‐loaded lipid nanoparticles with intense NIR‐II absorption was reported for PAI (Q. Chen et al, ). Figure a illustrates the synthesis procedure in which IR‐1061 dyes, CH, and DSPC were first dissolved in dichloromethane for rotary evaporation, and then modified with DSPE‐PEG 2000 .…”

Section: Pai With Exogenous Contrast Angetsmentioning

confidence: 99%

“…Figure a illustrates the synthesis procedure in which IR‐1061 dyes, CH, and DSPC were first dissolved in dichloromethane for rotary evaporation, and then modified with DSPE‐PEG 2000 . More details on these chemicals and reagents are given in Q. Chen, Chen, et al (). To overcome the inherent hydrophobicity of the IR‐1061 dye and expand its use for the biomedical application, Polipo‐IR NPs were synthesized by the rotary evaporation method.…”

Section: Pai With Exogenous Contrast Angetsmentioning

confidence: 99%

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Recent advances in photoacoustic contrast agents for in vivo imaging

Upputuri

Pramanik

2020

WIREs Nanomed Nanobiotechnol

118

119

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Photoacoustic imaging (PAI) is a noninvasive hybrid imaging modality offering rich optical contrast and high depth‐to‐resolution ratio deep‐tissue imaging. Endogenous chromophores present in the body such as hemoglobin, lipid, melanin, and so on provide strong photoacoustic contrast due to their strong light absorption in certain optical window. To enhance the performance of PAI further, researchers have developed several exogenous contrast agents such as metallic nanoparticles, carbon‐based nanomaterials, quantum dots, organic small molecules, semiconducting polymer nanoparticles, and so on. These exogenous contrast agents not only help improving the imaging contrast, but also make targeted molecular imaging possible. In this review article, we first discuss the state‐of‐the‐art PAI techniques with endogenous contrast mechanism. Later, we provide an overview of recent progress in the development of exogenous photoacoustic contrast agents for in vivo imaging applications. Finally, we present the pros/cons of the existing PA contrast agents along with future challenges of contrast agent‐based PAI for biomedical applications. This article is categorized under: Diagnostic Tools > Diagnostic Nanodevices Diagnostic Tools > In Vivo Nanodiagnostics and Imaging

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“…Therefore, in situ lightabsorbing agents are often used to selectively increase the thermal destruction of the targeted tumors and to reduce injury to normal tissues. [5][6][7][8] PTT uses high temperature to damage tumor cells by heating the lesions. [9][10][11] During treatment, the temperature can range from 40°C to more than 50°C, comparing hyperthermia (40-41°C), 12 moderate-temperature hyperthermia (42-45°C), 13 and thermal ablation, or high-temperature hyperthermia (>50°C).…”

Section: Introductionmentioning

confidence: 99%

Molecular Antenna-Sensitized Upconversion Nanoparticle for Temperature Monitored Precision Photothermal Therapy

Wei¹,

Liu²,

Pan³

et al. 2020

IJN

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Background: Photothermal therapy with accurate and real-time temperature detection is desired in clinic. Upconversion nanocrystals (UCNs) are candidate materials for simultaneous temperature detection and photothermal agents carrying. However, the weak luminescence and multiple laser excitations of UCNs limit their application in thermal therapy. Materials and Methods: NaYF 4 :Yb 3+ ,Er 3+ ,Nd 3+ , PL-PEG-NH 2 , IR-806 and folic acid are selected as structural components. A nanoprobe (NP) integrated with efficient photothermal conversion and sensitive temperature detection capabilities is synthesized for precise photothermal therapy. The probes are based on near-infrared upconversion nanocrystals doped with Yb, Er and Nd ions, which can be excited by 808 nm light. IR-806 dye molecules are modified on the surface as molecular antennas to strongly absorb near-infrared photons for energy transfer and conversion. Results: The results show that under an 808 nm laser irradiation upconversion luminescence of the nanocrystals is enhanced based on both the Nd ion absorption and the FRET energy transfer of IR-806. The luminescence ratio at 520 and 545 nm is calculated to accurately monitor the temperature of the nanoparticles. The temperature of the nanoprobes increases significantly through energy conversion of the molecular antennas. The nanoparticles are found successfully distributed to tumor cells and tumor tissue due to the modification of the biocompatible molecules on the surface. Tumor cells can be killed efficiently based on the photothermal effect of the NPs. Under the laser irradiation, temperature at mouse tumor site increases significantly, tissue necrosis and tumor cell death can be observed. Conclusion: Precision photothermal therapy can thus be achieved by highly efficient nearinfrared light absorption and accurate temperature monitoring, making it promising for tumor treatment, as well as the biological microzone temperature detection.

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Novel small molecular dye-loaded lipid nanoparticles with efficient near-infrared-II absorption for photoacoustic imaging and photothermal therapy of hepatocellular carcinoma

Abstract: The NIR-II PA and PTT nanoparticle based on the IR-1061 dye would benefit early diagnosis and treatment of HCC.

Cited by 48 publications

References 58 publications

<p>Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy</p>

<p>Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy</p>

Recent advances in photoacoustic contrast agents for in vivo imaging

<p>Molecular Antenna-Sensitized Upconversion Nanoparticle for Temperature Monitored Precision Photothermal Therapy</p>

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