Novel skeleton compound Allomyrinanoid A and two purine alkaloids from the adult of Allomyrina dichotoma L.

Niu, Lanlan; Gao, Jiayu; Li, Hai-Di; Liu, Junna; Yin, Weiping

doi:10.1016/j.bmcl.2015.12.012

Cited by 7 publications

(4 citation statements)

References 13 publications

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“…Moreover, it has been reported that extracts from A. dichotoma larvae offer various biological benefits such as antibacterial (Niu et al . ; Koyama et al . ; Sagisaka et al .…”

Section: Introductionmentioning

confidence: 98%

“…The Korean horn beetle, Allomyrina dichotoma (Linné 1771) is one of the major insects whose larvae have been consumed as traditional oriental medicine for liver disease and diabetes for a long time. Moreover, it has been reported that extracts from A. dichotoma larvae offer various biological benefits such as antibacterial (Niu et al 2016;Koyama et al 2006;Sagisaka et al 2001), anti-Alzheimer's disease (Kim et al 2014), anti-obesity (Kim et al 2016;Yoon et al 2015;Chung et al 2014), and antioxidant effects (Suh et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Establishment of a loop‐mediated isothermal amplification system for on‐site diagnosis of Oryctes rhinoceros nudivirus in Allomyrina dichotoma (Coleoptera: Scarabaeidae)

Lee

Park

2019

Entomological Research

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The Korean insect industry is rapidly developing, with the Korean horn beetle Allomyrina dichotoma as one of the most popular insects kept as pets. Korean horn beetles reared in local farms are suffering from Oryctes rhinoceros nudivirus (OrNV). In a nationwide investigation on the early death of young larvae due to this virus, 70 to 77% mortality was found. It was also confirmed that several larvae collected from some wild horn beetle habitats were infected with the virus. Thus, it was concluded that the virus could be transmitted vertically from an infected adult to the offspring and that the OrNV disease is a very serious threat to the wild horn beetle in Korea. With local farmers expecting that an early detection technique would be helpful for the quick removal of infected larvae, an on‐site diagnosis method for the viral disease using loop‐mediated isothermal amplification (LAMP) assay was thus tested. To avoid the DNA extraction process, the LAMP assay used a 50‐fold diluted hemolymph was set for diagnosis standardization and convenient on‐site application to infected larvae from local farms. A. dichotoma larvae were assayed for the OrNV infectivity with LAMP primers targeting the OrNV_gp102 gene. To evaluate the LAMP specificity, two bacterial pathogens, Bacillus thuringiensis and Serratia marcescens, causing disease in A. dichotoma were tested along with OrNV. The positive results were detected only from the OrNV‐infected larvae.

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“…Moreover, it has been reported that extracts from A. dichotoma larvae offer various biological benefits such as antibacterial (Niu et al . ; Koyama et al . ; Sagisaka et al .…”

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Establishment of a loop‐mediated isothermal amplification system for on‐site diagnosis of Oryctes rhinoceros nudivirus in Allomyrina dichotoma (Coleoptera: Scarabaeidae)

Lee

Park

2019

Entomological Research

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“…However, the pharmacological efficacy and useful medicinal ingredients of this beetle have not been thoroughly determined. Most studies that have been reported used extracts of A. dichotoma larvae and only a few compounds of the beetle were reported [11]. In addition, only three antibacterial peptides (defensin) and proteins (coleoptericin A and B) have been purified from the immunized hemolymph of A. dichotoma larvae [12,13].…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Activity of Antimicrobial Peptide Allomyrinasin Derived from the Dynastid Beetle, Allomyrina dichotoma

Lee¹,

Seo²,

Lee³

et al. 2019

Journal of Microbiology and Biotechnology

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In a previous work, we performed de novo RNA sequencing of Allomyrina dichotoma using next generation sequencing and identified several antimicrobial peptide candidates based on transcriptome analysis. Among them, a cationic antimicrobial peptide, allomyrinasin, was selected bioinformatically based on its physicochemical properties. Here, we assessed the antimicrobial and anti-inflammatory activities of allomyrinasin against microorganisms and mouse macrophage Raw264.7 cells. Allomyrinasin showed antimicrobial activities against various microbes and decreased the nitric oxide production of the lipopolysaccharide-induced Raw264.7 cells. Furthermore, quantitative RT-PCR and ELISA revealed that allomyrinasin reduced cytokine expression levels in the Raw264.7 cells. We also identified inducible nitric oxide synthase, cyclooxygenase-2 expression, and PGE 2 production through western blot analysis and ELISA. We confirmed that allomyrinasin bound to bacterial cell membranes via a specific interaction with lipopolysaccharides. Taken together, these data indicate that allomyrinasin has antimicrobial and anti-inflammatory activities as exemplified in lipopolysaccharide-induced Raw264.7 cells. We have provided a potentially useful antimicrobial peptide candidate that has both antimicrobial and anti-inflammatory activities.

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“…However, due to the low content levels and difficulty of separation, the assessment of the diversity of active molecules and the identification of novel structures small molecular remain a continuing challenge. Previously, the secondary metabolites with antibacterial features have been isolated from insects, invertebrates and arthropod in our group [4,5,6]. It especially highlights the antibiotic potentials of secondary metabolites, including low-abundance analogs in natural products.…”

Section: Introductionmentioning

confidence: 99%

Neo-5,22E-Cholestadienol Derivatives from Buthus martensii Karsch and Targeted Bactericidal Action Mechanisms

Yin

Gao

et al. 2018

Molecules

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The discovery and search for new antimicrobial molecules from insects and animals that live in polluted environments is a very important step in the scientific search for solutions to the current problem of antibiotic resistance. Previously, we have reported that the secondary metabolite with the antibacterial action discovered in scorpion. The current study further isolated three new compounds from Buthus martensii karsch, while compounds 1 and 2 possessed 5,22E-cholestadienol derivatives whose structure demonstrated broad spectrum bactericide activities. To explore the antibacterial properties of these new compounds, the result shows that compound 2 inhibited bacterial growth of both S. aureus and P. aeruginosa in a bactericidal rather than a bacteriostatic manner (MBC/MIC ratio ≤ 2). Similarly, with compound 1, a ratio of MBC/MIC ≤ 2 indicates bactericidal activity inhibited bacterial growth of P. aeruginosa. Remarkably, this suggests that two compounds can be classified as bactericidal agents against broad spectrum bactericide activities for 5,22E-cholestadienol derivatives from Buthus martensii karsch. The structures of compounds 1–3 were established by comprehensive spectra analysis including two-dimensional nuclear magnetic resonance (2D-NMR) and high-resolution electrospray ionization-mass spectrometry (HRESI-MS) spectra. The antibacterial mechanism is the specific binding (various of bonding forces between molecules) using compound 1 or 2 as a ligand based on the different receptor proteins’—2XRL or 1Q23—active sites from bacterial ribosome unit A, and thus prevent the synthesis of bacterial proteins. This unique mechanism avoids the cross-resistance issues of other antibacterial drugs.

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Novel skeleton compound Allomyrinanoid A and two purine alkaloids from the adult of Allomyrina dichotoma L.

Cited by 7 publications

References 13 publications

Establishment of a loop‐mediated isothermal amplification system for on‐site diagnosis of Oryctes rhinoceros nudivirus in Allomyrina dichotoma (Coleoptera: Scarabaeidae)

Establishment of a loop‐mediated isothermal amplification system for on‐site diagnosis of Oryctes rhinoceros nudivirus in Allomyrina dichotoma (Coleoptera: Scarabaeidae)

Anti-Inflammatory Activity of Antimicrobial Peptide Allomyrinasin Derived from the Dynastid Beetle, Allomyrina dichotoma

Neo-5,22E-Cholestadienol Derivatives from Buthus martensii Karsch and Targeted Bactericidal Action Mechanisms

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