Anti-Inflammatory Activity of Antimicrobial Peptide Allomyrinasin Derived from the Dynastid Beetle, Allomyrina dichotoma

Lee, Joon Ha; Seo, Minchul; Lee, Hwa Jeong; Baek, Minhee; Kim, In Woo; Kim, Sun Young; Kim, Mi Ae; Kim, Seong Hyun; Hwang, Jae Sam

doi:10.4014/jmb.1809.09031

Cited by 25 publications

(22 citation statements)

References 25 publications

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“…Hepatic fibrosis and inflammation are important risk factor for the progression of NAFLD [19]. As ADLE showed anti-hepatofibrotic and anti-inflammatory activity [10,20], we checked whether ADLE can improve fibrosis and inflammation in the liver of HFD-fed mice. As shown in Figure 4A, hepatic steatosis demonstrating single large fat droplets in the cytoplasm of hepatocytes and ballooning of hepatocytes was clearly observed in the HFD-fed mice, but ADLE or Met treatment blocked the hepatic steatosis.…”

Section: Resultsmentioning

confidence: 99%

Allomyrina dichotoma Larva Extract Ameliorates the Hepatic Insulin Resistance of High-Fat Diet-Induced Diabetic Mice

et al. 2019

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Allomyrina dichotoma larva is a nutritional-worthy future food resource and it contributes to multiple pharmacological functions. However, its antidiabetic effect and molecular mechanisms are not yet fully understood. Therefore, we investigated the hypolipidemic effect of A. dichotoma larva extract (ADLE) in a high-fat diet (HFD)-induced C57BL/6J mice model. Glucose tolerance and insulin sensitivity in HFD-induced diabetic mice significantly improved after ADLE administration for six weeks. The levels of serum triglyceride (TG), aspartate aminotransferase (AST), alanine transferase (ALT) activity, and lipid accumulation were increased in the liver of HFD-fed mice, but the levels were significantly reduced by the ADLE treatment. Moreover, hepatic fibrosis and inflammatory gene expression in the liver from HFD-treated mice were ameliorated by the ADLE treatment. Dephosphorylation of AMP-activated protein kinase (AMPK) by palmitate was inhibited in the ADLE treated HepG2 cells, and subsequently reduced expression of lipogenic genes, such as SREPBP-1c, ACC, and FAS were observed. The reduced expression of lipogenic genes and an increased phosphorylation of AMPK was also observed in the liver from diabetic mice treated with ADLE. In conclusion, ADLE ameliorates hyperlipidemia through inhibition of hepatic lipogenesis via activating the AMPK signaling pathway. These findings suggest that ADLE and its constituent bioactive compounds are valuable to prevent or treat hepatic insulin resistance in type 2 diabetes.

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Section: Resultsmentioning

confidence: 99%

Allomyrina dichotoma Larva Extract Ameliorates the Hepatic Insulin Resistance of High-Fat Diet-Induced Diabetic Mice

et al. 2019

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“…Nevertheless, only 305 of the 3070 HDP sequences deposited in the Antimicrobial Peptide Database (APD) are derived from insects ( Wang et al 2016 ). The HDP sequences and their biological activities have been described for several Coleoptera species, including: ( Allomyrina dichotoma ) ( Lee et al 2019 ), ( Octodonta nipae ) ( Meng et al 2019 ), Hylobius abietis ( Namara et al 2018 ), ( Nicrophorus vespilloides ) ( Vogel et al 2011 ), ( Tenebrio molitor ) ( Jacobs et al 2017 ), ( Calomera littoralis ) ( Rodríguez-García et al 2016 ), ( Protaetia brevitarsis seulensis ) ( Lee et al 2016 ), ( Tribolium castaneum ) ( Altincicek and Vilcinskas 2007 , Zou et al 2007 , Altincicek et al 2013 ), ( Holotrichia diomphalia ) ( Lee et al 1994 ), Zophobas atratus ( Bulet et al 1991 ), Allomyrina dichotoma ( Sagisaka et al 2001 ), ( Acalolepta luxuriosa ) ( Imamura et al 2009 ), and ( Sitophilus oryzae ) ( Login et al 2011 ). Our work further extends the list of HDP sequences to other species of Coleoptera.…”

Section: Resultsmentioning

confidence: 99%

New Insect Host Defense Peptides (HDP) From Dung Beetle (Coleoptera: Scarabaeidae) Transcriptomes

Ramírez

Osorio-Méndez

Arias

et al. 2021

Journal of Insect Science

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The Coleoptera Scarabaeidae family is one of the most diverse groups of insects on the planet, which live in complex microbiological environments. Their immune systems have evolved diverse families of Host Defense Peptides (HDP) with strong antimicrobial and immunomodulatory activities. However, there are several peptide sequences that await discovery in this group of organisms. This would pave the way to identify molecules with promising therapeutic potential. This work retrieved two sources of information: 1) De-novo transcriptomic data from two species of neotropical Scarabaeidae (Dichotomius satanas and Ontophagus curvicornis); 2) Sequence data deposited in available databases. A Blast-based search was conducted against the transcriptomes with a subset of sequences representative of the HDP. This work reports 155 novel HDP sequences identified in nine transcriptomes from seven species of Coleoptera: D. satanas (n = 76; 49.03%), O. curvicornis (n = 23; 14.83%), (Trypoxylus dichotomus) (n = 18; 11.61%), (Onthophagus nigriventris) (n = 10; 6.45%), (Heterochelus sp) (n = 6; 3.87%), (Oxysternon conspicillatum) (n = 18; 11.61%), and (Popillia japonica) (n = 4; 2.58%). These sequences were identified based on similarity to known HDP insect families. New members of defensins (n = 58; 37.42%), cecropins (n = 18; 11.61%), attancins (n = 41; 26.45%), and coleoptericins (n = 38; 24.52%) were described based on their physicochemical and structural characteristics, as well as their sequence relationship to other insect HDPs. Therefore, the Scarabaeidae family is a complex and rich group of insects with a great diversity of antimicrobial peptides with potential antimicrobial activity.

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“…Several studies have reported the efficacy of certain types of AMPs in killing S. pseudintermedius and M. canis in vitro ( Santoro and Maddox, 2014 ; Simonetti et al, 2014 ); however, there are little data about the application of newly discovered AMPs to in vitro antimicrobial assays for clinical isolates of S. pseudintermedius and M. canis from China and their activity in a S. pseudintermedius -induced mouse skin infection model. Two short synthetic peptides, namely, allomyrinasin and andricin B, identified from Allomyrina dichotoma and Andrias davidianus , and three other original short-chain peptides have been previously described as effective against staphylococcal and fungal strains, which may serve as good candidates for further research ( Pei et al, 2018 ; Bao et al, 2019 ; Bessa et al, 2019 ; Chaparro-Aguirre et al, 2019 ; Lee et al, 2019 ). In our present study, we confirmed that allomyrinasin and andricin B exhibited potent antimicrobial activities by the disruption of microbial cell membrane.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Short-Chain Antimicrobial Peptides With Combined Antimicrobial and Anti-inflammatory Bioactivities for the Treatment of Zoonotic Skin Pathogens From Canines

Tang

Yang

et al. 2021

Front. Microbiol.

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The incidence of zoonotic Staphylococcus pseudintermedius and Microsporum canis infections is rapidly growing worldwide in the context of an increasing frequency of close contact between animals and humans, presenting challenges in both human and veterinary medicine. Moreover, the development of microbial resistance and emergence of recalcitrant biofilms, accompanied by the insufficiency of new antimicrobial agents, have become major obstacles in treating superficial skin infections caused by various microbes including S. pseudintermedius and M. canis. Over recent years, the prospects of antimicrobial peptides as emerging antimicrobials to combat microbial infections have been demonstrated. In our study, two novel short-chain peptides, namely, allomyrinasin and andricin B, produced by Allomyrina dichotoma and Andrias davidianus, were revealed to exhibit potent antimicrobial efficacy against clinical isolates of S. pseudintermedius and M. canis with remarkable and rapid fungicidal and bactericidal effects, while allomyrinasin exhibited inhibition of biofilm formation and eradication of mature biofilm. These peptides displayed synergistic activity when combined with amoxicillin and terbinafine against S. pseudintermedius and M. canis. Cytoplasmic leakage via cytomembrane permeabilization serves as a mechanism of action. Extremely low hemolytic activity and serum stability in vitro, as well as superior anti-infective efficacy in reducing bacterial counts and relieving the inflammatory response in vivo, were detected. The potent antibacterial, antifungal, and anti-inflammatory activities of allomyrinasin and andricin B might indicate promising anti-infective alternatives for the treatment of S. pseudintermedius and M. canis infections in the context of human and veterinary medicine.

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Anti-Inflammatory Activity of Antimicrobial Peptide Allomyrinasin Derived from the Dynastid Beetle, Allomyrina dichotoma

Cited by 25 publications

References 25 publications

Allomyrina dichotoma Larva Extract Ameliorates the Hepatic Insulin Resistance of High-Fat Diet-Induced Diabetic Mice

Allomyrina dichotoma Larva Extract Ameliorates the Hepatic Insulin Resistance of High-Fat Diet-Induced Diabetic Mice

New Insect Host Defense Peptides (HDP) From Dung Beetle (Coleoptera: Scarabaeidae) Transcriptomes

Evaluation of Short-Chain Antimicrobial Peptides With Combined Antimicrobial and Anti-inflammatory Bioactivities for the Treatment of Zoonotic Skin Pathogens From Canines

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