Novel single‐step multiplex real‐time polymerase chain reaction assay for simultaneous quantification of hepatitis virus <scp>A</scp>, <scp>B</scp>, <scp>C</scp>, and <scp>E</scp> in serum

Irshad, Mohammad; Ansari, M. A.; Irshad, Khushboo; Lingaiah, Raghavendra

doi:10.1111/jgh.12302

Cited by 13 publications

(7 citation statements)

References 32 publications

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“…Although some rapid POC tests can detect HBV and HCV simultaneously with human immunodeficiency virus or syphilis, few POC tests can detect HBV and HCV simultaneously with HAV. The simultaneous detection of hepatitis viruses including HAV is generally performed with array technology or nucleotide amplification tests (NATs) …”

Section: Introductionmentioning

confidence: 57%

“…The simultaneous detection of hepatitis viruses including HAV is generally performed with array technology or nucleotide amplification tests (NATs). 15,16 The EuDx-HE (A,B,C) kit (EUDIPIA Inc., Cheongju, Republic of Korea), is a newly developed rapid POC test for the simultaneous qualitative detection of anti-HAV IgM, HBsAg, and anti-HCV IgG in 15 minutes using serum samples. It is manually performed, visually interpreted, and works on the basis of immunochromatographic methods with lateral flow.…”

mentioning

confidence: 99%

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Analytical performance of newly developed rapid point‐of‐care test for the simultaneous detection of hepatitis A, B, and C viruses in serum samples

Kweon

Lim

Kim

et al. 2019

Journal of Medical Virology

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Background Although immunoassays play a major role in the detection of hepatitis viruses, there is a need for a point‐of‐care (POC) test. We evaluated the EuDx‐HE (A,B,C) kit (EUDIPIA, Cheongju, Korea), which detects anti‐hepatitis A virus (HAV) immunoglobulin M (IgM), hepatitis B virus surface antigen (HBsAg), and anti‐hepatitis C virus (HCV) immunoglobulin G (IgG) simultaneously using an immunochromatographic method within 15 minutes. Methods A total of 1581 serum samples including 57, 477, and 451 samples positive for anti‐HAV IgM, HBsAg, and anti‐HBV IgG, respectively, were analyzed. We investigated the diagnostic accuracy of the EuDx‐HE (A,B,C) kit by comparison with SD BIOLINE POC kits (Abbott, Chicago, IL) using Architect immunoassays as a reference method. Results For anti‐HAV IgM and HBsAg detection, the EuDx‐HE (A,B,C) kit showed a higher sensitivity and a slightly lower specificity than the SD BIOLINE kit. For anti‐HCV IgG detection, the EuDx‐HE (A,B,C) kit had a higher sensitivity and a higher specificity than the SD BIOLINE kit. The agreement for positivity between the POC tests was >89.47%, with κ values of 0.844, 0.941, and 0.943 for HAV, HBV, and HCV, respectively. Conclusion The EuDx‐HE (A,B,C) kit showed an acceptable clinical performance for detecting anti‐HAV IgM, HBsAg, and anti‐HCV.

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Section: Introductionmentioning

confidence: 57%

mentioning

confidence: 99%

Analytical performance of newly developed rapid point‐of‐care test for the simultaneous detection of hepatitis A, B, and C viruses in serum samples

Kweon

Lim

Kim

et al. 2019

Journal of Medical Virology

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“…Besides superior stability and reproducibility (Table 1), the detection limit of the multiplex real-time PCR was 10 copies per reaction for detection of HAV and HEV, respectively, suggesting that it has the same sensitivity as HAV/HEV uniplex real-time PCR [11,12]. Compared with a similar study by other researchers [16], the duplex real-time PCR developed in this study was a little less sensitive than the simultaneous quantification system of hepatitis virus A, B, C and E established by Irshad M. et al ., the detection limits of which were 280 copies/mL for HAV and 300 copies/mL for HEV. For our interest, for a similar concentration of HAV and HEV templates, multiplex real-time PCR gave very different Ct values (Table 1 and Table 2), indicating the different PCR efficiency of HAV and HEV templates during amplification.…”

Section: Discussionmentioning

confidence: 85%

Multiplex Hydrolysis Probe Real-Time PCR for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus

Qiu

Cao

et al. 2014

IJMS

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Detection of hepatitis viral infections has traditionally relied on the circulating antibody test using the enzyme-linked immunosorbent assay. However, multiplex real-time PCR has been increasingly used for a variety of viral nucleic acid detections and has proven to be superior to traditional methods. Hepatitis A virus (HAV) and hepatitis E virus (HEV) are the major causes of acute hepatitis worldwide; both HAV and HEV infection are a main public health problem. In the present study, a one-step multiplex reverse transcriptase quantitative polymerase chain reaction assay using hydrolysis probes was developed for simultaneously detecting HAV and HEV. This novel detection system proved specific to the target viruses, to be highly sensitive and to be applicable to clinical sera samples, making it useful for rapid, accurate and feasible identification of HAV and HEV.

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“…For the detection of hepatitis A, B, C and E nucleic acids in sera of infected patients, a single step-multiplex RT-PCR was created in which the conserved regions of all the viral genomes were used as target sequence for amplification and the method was found to be rapid, sensitive, precise and reproducible in nature. The detection limit was found to be 280 copies/ml for hepatitis A virus; for hepatitis B 290copies/ml; for hepatitis C 30copies/ml and 300 copies/ml for hepatitis E in a single tube assay system 114 .…”

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confidence: 90%

Hepatitis E: Current Status in India and Other Asian Countries

Kumar¹,

Kamar²,

Kumar³

2019

J Pure Appl Microbiol

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Six types of hepatitis infections are accounted for the inflammation of liver in human throughout the world and assigned the letter A, B, C, D, E and G. Out of these, Hepatitis E, caused by Hepatitis E virus (HEV), currently categorized as a member of the genus Hepevirus in the family Hepeviridae. Hepatitis E is considered highly significant, on account of its predominance in both developed and developing nations, due to poor sanitation and cleanliness condition related to drinking water. This review focuses on general epidemiological study update of hepatitis E virus in India and rest of Asia for the selected period and also throws light on some diagnostic approaches available for the same. An extensive literature search was performed with published work and search in PubMed using term "HEV" combined with "country name" restricting the search publication between year 2008 to 2018 for India and 2013 to 2018 for the rest of Asia. The data was analyzed in detail to meet the requirements of the objectives. Study showed that in India the major causes of Hepatitis E was water contaminated with fecal matter. Reports from East and Southeast Asia signified the role of animal reservoir especially the pigs for HEV. Whereas in Indian neighboring countries like Nepal, Pakistan and Western Asia the major cases of Hepatitis E were associated with blood transfusion and pregnancy related cases. Majority of the diagnostic approaches used in India at medical facilities are based on anti-HEV IgM detection. However, the molecular based approaches played very crucial role in HEV detection. It is observed that the incidence of HEV is gradually expanding and its objective range is not only just developing countries, but also showing an enhancement. In overall, there is need for improvements in detection of HEV disease and methodologies used for the development of HEV vaccines.

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Novel single‐step multiplex real‐time polymerase chain reaction assay for simultaneous quantification of hepatitis virus A, B, C, and E in serum

Abstract: Present multiplex real-time PCR is the first report on single-step nucleic acid detection of HAV, HBV, HCV, and HEV in sera samples. It is an alternate diagnostic assay for common use in laboratories analyzing viral hepatitis cases.

Cited by 13 publications

References 32 publications

Analytical performance of newly developed rapid point‐of‐care test for the simultaneous detection of hepatitis A, B, and C viruses in serum samples

Analytical performance of newly developed rapid point‐of‐care test for the simultaneous detection of hepatitis A, B, and C viruses in serum samples

Multiplex Hydrolysis Probe Real-Time PCR for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus

Hepatitis E: Current Status in India and Other Asian Countries

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