Multiplex Hydrolysis Probe Real-Time PCR for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus

Qiu, Feng; Cao, Jun; Su, Qiudong; Yao, Yi; Bi, Shengli

doi:10.3390/ijms15069780

Cited by 8 publications

(2 citation statements)

References 17 publications

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“…Further developments include multiplex RT-qPCR assays, which enable simultaneous detection of HEV and HAV (Tahk et al, 2011;Qiu et al, 2014), or even the parallel detection of 19 humanpathogenic viruses including HEV-3 strains using a nanofluidic RT-qPCR system (Coudray-Meunier et al, 2016). In addition, multiplex assays were developed that allow the simultaneous detection of HEV and internal amplification controls, thus assessing the amplification efficiency of each reaction (Martin-Latil et al, 2012;Vasickova et al, 2012).…”

Section: Nucleic Acid Amplification Techniquesmentioning

confidence: 99%

Public health risks associated with hepatitis E virus (HEV) as a food‐borne pathogen

Ricci¹,

Allende²,

Bolton³

et al. 2017

EFS2

111

107

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Hepatitis E virus (HEV) is an important infection in humans in EU/EEA countries, and over the last 10 years more than 21,000 acute clinical cases with 28 fatalities have been notified with an overall 10-fold increase in reported HEV cases; the majority (80%) of cases were reported from France, Germany and the UK. However, as infection in humans is not notifiable in all Member States, and surveillance differs between countries, the number of reported cases is not comparable and the true number of cases would probably be higher. Food-borne transmission of HEV appears to be a major route in Europe; pigs and wild boars are the main source of HEV. Outbreaks and sporadic cases have been identified in immune-competent persons as well as in recognised risk groups such as those with preexisting liver damage, immunosuppressive illness or receiving immunosuppressive treatments. The opinion reviews current methods for the detection, identification, characterisation and tracing of HEV in food-producing animals and foods, reviews literature on HEV reservoirs and food-borne pathways, examines information on the epidemiology of HEV and its occurrence and persistence in foods, and investigates possible control measures along the food chain. Presently, the only efficient control option for HEV infection from consumption of meat, liver and products derived from animal reservoirs is sufficient heat treatment. The development of validated quantitative and qualitative detection methods, including infectivity assays and consensus molecular typing protocols, is required for the development of quantitative microbial risk assessments and efficient control measures. More research on the epidemiology and control of HEV in pig herds is required in order to minimise the proportion of pigs that remain viraemic or carry high levels of virus in intestinal contents at the time of slaughter. Consumption of raw pig, wild boar and deer meat products should be avoided.

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Section: Nucleic Acid Amplification Techniquesmentioning

confidence: 99%

Public health risks associated with hepatitis E virus (HEV) as a food‐borne pathogen

Ricci¹,

Allende²,

Bolton³

et al. 2017

EFS2

111

107

View full text Add to dashboard Cite

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“…Researchers have shown that positive anti-HAV IgM existed among individuals who did not have HAV infection (24)(25)(26), and patients who had other medical conditions could be misdiagnosed based on the anti-HAV IgM test. Moreover, convalescent persons could also be reported as new cases since it usually takes 3 to 6 months for IgM to become undetectable (27) and the persistence of IgM for years after the primary infection also existed (28). Since performing sequencing for these patients would be a waste of the limited human and financial resources, more accurate and specific criteria for HepA sequencing should be developed.…”

Section: Discussionmentioning

confidence: 99%

Implementing sequencing-based surveillance in developing countries: findings from a pilot rollout for hepatitis A in China

Zhou¹,

Zhang²,

Lv³

et al. 2021

Ann Transl Med

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Background: The emergence of SARS-Cov2 variants has highlighted the need to implement sequencingbased surveillance in developing countries for early response to mutant viruses of concern. However, limited information on how to implement sequencing-based surveillance is available, and the feasibility and performance of this new type of surveillance are still in question.Methods: To understand the challenges with the implementation and to promote sequencing-based surveillance, we reported findings from a pilot for hepatitis A in five sentinel provinces in China as an example of sequencing-based surveillance implementation. The performance of the surveillance system was evaluated by indicators related to acceptability, data quality, simplicity, utility, and timeliness. We use a scale from 1 to 3 was used to provide a score for each aspect.Results: During the pilot, 306 cases of hepatitis A were reported, and 49.79% of samples were available for sequencing. Eleven genomic clusters were found, of which seven clusters were potentially related to a foodborne outbreak oyster based on identical viral sequence and epidemiologic investigations. The greatest strength of the system was its simplicity (Score: 2.63). The acceptability (Score: 2.0) and utility (Score: 2.33) were modest, but data quality (Score: 1.75) and timeliness (Score: 1.75) were the main challenges.Conclusions: Overall, the system performed satisfactorily and proved to be useful for virological characterization of cases and early outbreak detection, with a great potential for scale-up. Further efforts are required to address financial and human resource constraints and inadequate support among physicians.Education should be given to health care professionals to improve the data quality. The establishment of decentralized surveillance networks can be an approach to improve timeliness for emerging infections.

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Target Induced-DNA strand displacement reaction using gold nanoparticle labeling for hepatitis E virus detection

Ngamdee

Yin

Vongpunsawad

et al. 2020

Analytica Chimica Acta

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Multiplex Hydrolysis Probe Real-Time PCR for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus

Cited by 8 publications

References 17 publications

Public health risks associated with hepatitis E virus (HEV) as a food‐borne pathogen

Public health risks associated with hepatitis E virus (HEV) as a food‐borne pathogen

Implementing sequencing-based surveillance in developing countries: findings from a pilot rollout for hepatitis A in China

Target Induced-DNA strand displacement reaction using gold nanoparticle labeling for hepatitis E virus detection

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