Novel sequences and epitopes of diagnostic value derived from the <i>Anisakis simplex</i> Ani s 7 major allergen*

Rodríguez, Esperanza; Anadón, Arturo; García-Bodas, Elena; Romarís, F.; Iglesias, R.; Gárate, Teresa; Ubeira, Florencio M.

doi:10.1111/j.1398-9995.2007.01564.x

Cited by 48 publications

(48 citation statements)

References 25 publications

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“…The occurrence of human anisakiasis has risen dramatically since it was first described almost 50 years ago with a number of human and environmental factors likely contributing to the rise (van Thiel et al 1960). The advent of the endoscope has undoubtedly led to the detection of more cases of human anisakiasis and this, together with advances in the immunological diagnosis of the disease, should see that this trend will continue (Rodríguez et al 2008). Changing dietary trends may also have contributed to the spread of human anisakiasis.…”

Section: (B) Anisakiasismentioning

confidence: 99%

Zoonotic helminth infections with particular emphasis on fasciolosis and other trematodiases

Robinson

Dalton

2009

Phil. Trans. R. Soc. B

142

109

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Zoonotic infections are among the most common on earth and are responsible for .60 per cent of all human infectious diseases. Some of the most important and well-known human zoonoses are caused by worm or helminth parasites, including species of nematodes (trichinellosis), cestodes (cysticercosis, echinococcosis) and trematodes (schistosomiasis). However, along with social, epidemiological and environmental changes, together with improvements in our ability to diagnose helminth infections, several neglected parasite species are now fast-becoming recognized as important zoonotic diseases of humans, e.g. anasakiasis, several fish-borne trematodiasis and fasciolosis. In the present review, we discuss the current disease status of these primary helminth zoonotic infections with particular emphasis on their diagnosis and control. Advances in molecular biology, proteomics and the release of helminth genome-sequencing project data are revolutionizing parasitology research. The use of these powerful experimental approaches, and their potential benefits to helminth biology are also discussed in relation to the future control of helminth infections of animals and humans.

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Section: (B) Anisakiasismentioning

confidence: 99%

Zoonotic helminth infections with particular emphasis on fasciolosis and other trematodiases

Robinson

Dalton

2009

Phil. Trans. R. Soc. B

142

109

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“…Ani s 5 is not a major allergen of A. simplex (158). Ani s 7 is a glycoprotein present in ES and is the target of the UA3 MAb, and it seems to be recognized by 100% of allergic patients (228).…”

Section: Anisakis Allergensmentioning

confidence: 99%

Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity

2008

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SUMMARY Infection of humans with the nematode worm parasite Anisakis simplex was first described in the 1960s in association with the consumption of raw or undercooked fish. During the 1990s it was realized that even the ingestion of dead worms in food fish can cause severe hypersensitivity reactions, that these may be more prevalent than infection itself, and that this outcome could be associated with food preparations previously considered safe. Not only may allergic symptoms arise from infection by the parasites (“gastroallergic anisakiasis”), but true anaphylactic reactions can also occur following exposure to allergens from dead worms by food-borne, airborne, or skin contact routes. This review discusses A. simplex pathogenesis in humans, covering immune hypersensitivity reactions both in the context of a living infection and in terms of exposure to its allergens by other routes. Over the last 20 years, several studies have concentrated on A. simplex antigen characterization and innate as well as adaptive immune response to this parasite. Molecular characterization of Anisakis allergens and isolation of their encoding cDNAs is now an active field of research that should provide improved diagnostic tools in addition to tools with which to enhance our understanding of pathogenesis and controversial aspects of A. simplex allergy. We also discuss the potential relevance of parasite products such as allergens, proteinases, and proteinase inhibitors and the activation of basophils, eosinophils, and mast cells in the induction of A. simplex-related immune hypersensitivity states induced by exposure to the parasite, dead or alive.

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“…In comparing the sensitivities and specificities of the Ani s 1 ELISA, Ani s 7 ELISA, and CAP FEIA methods, we considered a serum to be truly positive (gold standard) when it tested positive by Ani s 1 ELISA, Ani s 7 ELISA, or both methods. This criterion was based on the following: (i) Ani s 7 and, probably, Ani s 1 are excretory-secretory allergens that are released and recognized by the host immune system only in the course of an Anisakis infection (3, 6); (ii) both allergens are highly specific, as deduced from their primary amino acid sequences, which have no significant homologies with any recognized human allergen; (iii) these methods have proven high sensitivities, as they are reported to recognize about 85% and 100% of well-characterized positive sera, respectively (8,16,23,31); and (iv) it is expected that the combined use of the Ani s 1 and Ani s 7 allergens in ELISA will increase the individual sensitivity of each …”

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confidence: 99%

“…However, the assay was found to be somewhat limited, mainly due to the difficulty in obtaining large amounts of parasites and antigen uniformity between batches. Interestingly, the major Anisakis allergen recognized by MAb UA3 (Ani s 7) was recently characterized (31), and an internal portion of its sequence (t-Ani s 7) was cloned and expressed in Escherichia coli. Previous results obtained with this allergen as a target in indirect ELISA showed it to be highly specific (3), with a similar sensitivity to that obtained with UA3-capture ELISA (31).…”

mentioning

confidence: 99%

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Diagnosing Human Anisakiasis: Recombinant Ani s 1 and Ani s 7 Allergens versus the UniCAP 100 Fluorescence Enzyme Immunoassay

Anadón

Rodríguez

Gárate

et al. 2010

Clin Vaccine Immunol

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Novel sequences and epitopes of diagnostic value derived from the Anisakis simplex Ani s 7 major allergen*

Abstract: In addition to their clear value for serodiagnosis of human anisakiasis, the nature of the novel sequences and epitopes identified in the Ani s 7 allergen are of interest for a better understanding of the mechanisms operating in Anisakis-induced allergy.

Cited by 48 publications

References 25 publications

Zoonotic helminth infections with particular emphasis on fasciolosis and other trematodiases

Zoonotic helminth infections with particular emphasis on fasciolosis and other trematodiases

Anisakis simplex: from Obscure Infectious Worm to Inducer of Immune Hypersensitivity

Diagnosing Human Anisakiasis: Recombinant Ani s 1 and Ani s 7 Allergens versus the UniCAP 100 Fluorescence Enzyme Immunoassay

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